LOCUS       40924_20811        4785 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pG-Sable, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Foureau E, Courdavault V, Simkin AJ, Sibirny AA, Creche J, 
            Giglioli-Guivarc'h N, Clastre M, Papon N.
  TITLE     Transformation of Candida guilliermondii wild-type strains using the
            Staphylococcus aureus MRSA 252 ble gene as a phleomycin-resistant 
            marker
  JOURNAL   FEMS Yeast Res. 13 (3), 354-358 (2013)
  PUBMED    23374647
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Papon N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2013) Ea2106 Biomolecules et Biotechnologie 
            Vegetales, Universite Francois Rabelais de Tours, 31 Avenue Monge, 
            Tours, Centre F-37200, France
REFERENCE   3  (bases 1 to 4785)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4785)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res."; date: "2013"; volume: "13"; issue: "3"; pages: "354-358"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-JAN-2013) Ea2106 Biomolecules et Biotechnologie Vegetales, 
            Universite Francois Rabelais de Tours, 31 Avenue Monge, Tours, 
            Centre F-37200, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      31..656
                     /note="derived from C. guilliermondii phosphoglycerate
                     kinase (PGK) 5'UTR"
                     /regulatory_class="promoter"
     CDS             675..1073
                     /codon_start=1
                     /product="glyoxalase"
                     /EC_number="4.4.1.5"
                     /label=glyoxalase
                     /note="confers resistance to phleomycin and zeocin; derived
                     from Staphylococcus aureus MRSA 252 ble"
                     /protein_id="AGI99512.1"
                     /translation="MLQSIPALPVGDIKKSIGFYCDKLGFTLVHHEDGFAVLMCNEVRI
                     HLWEASDEGWRSRSNDSPVCTGAESFIAGTASCRIEVEGIDELYQHIKPLGILHPNTSL
                     KDQWWDERDFAVIDPDNNLISFFQQIKS"
     regulatory      1089..1746
                     /note="derived from C. guilliermondii phosphoglycerate
                     kinase gene (PGK) 3'UTR"
                     /regulatory_class="terminator"
     promoter        complement(1850..1868)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1886..1902)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1910..1926)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1934..1964)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1979..2000)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2288..2876)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3050..3907)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3908..4012)
                     /label=AmpR promoter
     rep_origin      complement(4090..4545)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     4686..4702
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4709..4727
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4758..4767
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4762..4771
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggtacctcta gaatcgattg atcagtcgac ggaggagtct tccgcaagag ccaaaacttg
       61 tttgaatcct tcgggatgac gaagaaaatc gtcctgggcc ttggggttgt tctggaccgc
      121 aatggcaatg catgaagcgg catacactcg caacgaatct ggcactttgg tgtccaattg
      181 cgcaattaaa ctgggccaaa gcttcaaatt ttcaatattg ttcgcattat ccatgttttc
      241 aatgagcatt tcaaagtttt caaacgaaac ttctttattt tccatcagag cttcgtcgct
      301 ggaaattaac gtcatggcat gtttcataag cgccggttca tctggcccgc caaaaagctg
      361 ctcaagcatc ttggggtccg gctggccgat cttggccatg gcctctttgt cgccggactg
      421 ctgggcgatc gaccactgta ataatttgtc catgaagatt aatgaagatt actgaagatg
      481 aacctcgaac acccaagcac cggtgagttg agtctctcca caacttcggg tgtgcggatg
      541 taactagccc ggggtggtcg cgtccggccc caaatggcga gatccggtgg tgcggtatat
      601 aaggagcagc accctgctgc ctatcgaaaa taatccgatc gattgctaga attaccggat
      661 ccgctagcct cgagatgtta cagtctatcc cggcattgcc agtcggggat attaaaaaga
      721 gtataggttt ttattgcgat aaactaggtt tcactttggt tcaccatgaa gatggattcg
      781 cagttctaat gtgtaatgag gttcggattc atctatggga ggcaagtgat gaaggctggc
      841 gctctcgtag taatgattca ccggtttgta caggtgcgga gtcgtttatt gctggtactg
      901 ctagttgccg cattgaagta gagggaattg atgaattata tcaacatatt aagcctttgg
      961 gcattttgca ccccaataca tcattaaaag atcagtggtg ggatgaacga gactttgcag
     1021 taattgatcc cgacaacaat ttgattagct tttttcaaca aataaaaagc taactgcagg
     1081 gcgcctaaga atatacgtcg ttattttgtg ttatacactc agctacaatt tcgatttccc
     1141 gctcatgatg aggccccatg cttcctctgt caactcctcg tacttgtcac cattcaacca
     1201 gtagatcaca tcggtgccgc tttctccctt gggaagtttt tggttcttaa attggttctt
     1261 gggcaccttg tggttatgtg aagcttgaat ctcagacccc aacttgataa actggggaac
     1321 agcatatctg ggaagcgatt tgctaatatg attgtagatc tgcgccaatg cttgagggtg
     1381 cttacttgga tcattcaact cgtccacaag atctataacg gcaaacccag ctctaccctc
     1441 atggttgggc actcgcacac caacaaccac cgactgtttc atggccttgg aacccatcaa
     1501 ctcattttca acttctgtag cagacacatt ttctgatttc caccggaaag tgtctcccaa
     1561 tctatcaaca aagtacaaca agccgtcctg gtccatcttg agcaaatcgc ctgttctgaa
     1621 ccaggcatcg tcttttttga aaacgctacg aacaagtttt gaagaagtag ccttgttgtt
     1681 cccgtagtac ccttggaacg actcctctgg tttcttggca ttcaagatct tcatcaacaa
     1741 ctcgccggta cctctagaat cgattgatca atcactagtg aattcgcggc cgcctgcagg
     1801 tcgaccatat gggagagctc ccaacgcgtt ggatgcatag cttgagtatt ctatagtgtc
     1861 acctaaatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     1921 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     1981 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     2041 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     2101 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     2161 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     2221 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2281 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     2341 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     2401 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     2461 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     2521 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     2581 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     2641 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     2701 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     2761 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2821 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     2881 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     2941 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     3001 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     3061 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     3121 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     3181 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     3241 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     3301 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     3361 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     3421 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     3481 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     3541 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     3601 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     3661 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     3721 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     3781 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3841 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3901 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3961 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     4021 cccgaaaagt gccacctgat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     4081 cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     4141 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     4201 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     4261 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     4321 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     4381 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     4441 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     4501 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag
     4561 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     4621 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     4681 acgttgtaaa acgacggcca gtgaattgta atacgactca ctatagggcg aattgggccc
     4741 gacgtcgcat gctcccggcc gccatggcgg ccgcgggaat tcgat
//
