LOCUS       62056_10750        5917 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..457
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        484..588
                     /label=AmpR promoter
     CDS             589..1446
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1620..2208
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  complement(2513..2737)
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2807..3337)
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(3526..3554)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3906..3997
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             4043..4060
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             4061..5158
                     /codon_start=1
                     /label=MBP
                     /note="maltose binding protein from E. coli"
                     /translation="KTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE
                     KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPAAAFQDKLYPFTWDAVRYNGKLI
                     AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYAAGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEHAFNHGET
                     AMTINGPWAWSNIDTSAVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE
                     NYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRVAATMENAQKGEIMPNIPQMSAFW
                     YAVRTAVINAASGRQTVDAALAAAQT"
     CDS             5162..5182
                     /codon_start=1
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
                     /translation="ENLYFQG"
     polyA_signal    5407..5541
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  complement(5570..5735)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagacca gccgcgtaac
     2461 ctggcaaaat cggttacggt tgagtaataa atggatgccc tgcgtaagcg ggtgtgggcg
     2521 gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa gtcttaaact
     2581 agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc atactggact
     2641 tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa
     2701 gaggggcgtg gccaagggca tggtaaagac tatattcgcg gcgttgtgac aatttaccga
     2761 acaactccgc ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg
     2821 ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag agagccactg
     2881 cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc gcgttggcct
     2941 catgcttgag gagattgatg agcgcggtgg caatgccctg cctccggtgc tcgccggaga
     3001 ctgcgagatc atagatatag atctcactac gcggctgctc aaacctgggc agaacgtaag
     3061 ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac
     3121 tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag gtggctacgt
     3181 ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact tggtcagggc
     3241 cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt ttagggcgac
     3301 tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt tgctgctcca taacatcaaa
     3361 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa
     3421 aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc
     3481 aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt acgaaccgaa
     3541 caggcttatg tcaactgggt tcgtgccttc atccgtttcc acggtgtgcg tcacccggca
     3601 accttgggca gcagcgaagt cgaggcattt ctgtcctggc tggcgaacga gcgcaaggtt
     3661 tcggtctcca cgcatcgtca ggcattggcg gccttgctgt tcttctacgg caaggtgctg
     3721 tgcacggatc tgccctggct tcaggagatc ggaagacctc ggccgtcgcg gcgcttgccg
     3781 gtggtgctga ccccggatga agtggttcgc atcctcggtt ttctggaagg cgagcatcgt
     3841 ttgttcgccc aggactctag ctatagttct agtggttggc tacgtatact ccggaatatt
     3901 aatagatcat ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg
     3961 ttttcgtaac agttttgtaa taaaaaaacc tataaatatt ccggattatt cataccgtcc
     4021 caccatcggg cgcggatcca tgcaccatca ccatcaccat aaaaccgaag aaggtaaact
     4081 ggtaatctgg attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt
     4141 cgagaaagat accggaatta aagtcaccgt tgagcatccc gataaactgg aagagaaatt
     4201 cccacaggtt gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt
     4261 tggtggctac gctcaatctg gcctgttggc tgaaatcacc ccggccgcag cgttccagga
     4321 caagctgtat ccgtttacct gggatgccgt gcgctacaac ggcaagctga ttgcttaccc
     4381 gatcgctgtt gaagcgttgt ccctgattta taacaaggat ctgctgccga acccgccaaa
     4441 aacctgggaa gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct
     4501 gatgttcaac ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta
     4561 tgcgttcaag tatgcagccg gaaagtacga cattaaagac gtgggcgtgg ataacgctgg
     4621 cgcgaaagcg ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga
     4681 caccgattac tccatcgcag aacatgcctt taatcatggc gaaacagcga tgaccatcaa
     4741 cggcccgtgg gcatggtcca acatcgacac cagcgcagtg aattatggtg taacggtgct
     4801 gccgaccttc aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa
     4861 cgccgccagt ccgaacaaag agctggcaaa agagttcctc gaaaactacc tgctgactga
     4921 tgaaggtctg gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta
     4981 cgaggaagag ttggtgaaag atccacgtgt tgccgccaca atggaaaacg cccagaaagg
     5041 tgaaatcatg cccaacatcc cgcagatgtc cgctttctgg tatgccgtgc gcactgcggt
     5101 gattaacgcc gccagcggtc gtcagactgt cgatgcagcc ctggcagccg cgcagactaa
     5161 tgagaacctg tacttccagg gcggatccgc tagcgaattc aaaggcctac gtcgacgagc
     5221 tcactagtcg cggccgcttt cgaatctaga gcctgcagtc tcgaggcatg cggtaccaag
     5281 cttgtcgaga agtactagag gatcataatc agccatacca catttgtaga ggttttactt
     5341 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     5401 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5461 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     5521 gtatcttatc atgtctggat ctgatcactg cttgagccta ggagatccga accagataag
     5581 tgaaatctag ttccaaacta ttttgtcatt tttaattttc gtattagctt acgacgctac
     5641 acccagttcc catctatttt gtcactcttc cctaaataat ccttaaaaac tccatttcca
     5701 cccctcccag ttcccaacta ttttgtccgc ccacagcggg gcatttttct tcctgttatg
     5761 tttttaatca aacatcctgc caactccatg tgacaaaccg tcatcttcgg ctactttttc
     5821 tctgtcacag aatgaaaatt tttctgtcat ctcttcgtta ttaatgtttg taattgactg
     5881 aatatcaacg cttatttgca gcctgaatgg cgaatgg
//
