LOCUS       62056_11035        4212 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..242
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             255..523
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     sig_peptide     573..632
                     /label=IL-2 signal sequence
                     /note="human interleukin-2 signal sequence"
     CDS             654..1349
                     /codon_start=1
                     /label=Fc tag
                     /note="CH2 and CH3 domains of the mouse IgG2a heavy chain
                     plus the hinge region"
                     /translation="PRGPTIKPCPPCKCPAPNLLGGPSVFIFPPKIKDVLMISLSPIVT
                     CVVVDVSEDDPDVQISWFVNNVEVHTAQTQTHREDYNSTLRVVSALPIQHQDWMSGKEF
                     KCKVNNKDLPAPIERTISKPKGSVRAPQVYVLPPPEEEMTKKQVTLTCMVTDFMPEDIY
                     VEWTNNGKTELNYKNTEPVLDSDGSYFMYSKLRVEKKNWVERNSYSCSVVHEGLHNHHT
                     TKSFSRTPGK"
     polyA_signal    complement(1377..1498)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    complement(1602..1996)
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(2013..2384)
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     enhancer        complement(2977..3280)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3360..3948)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    4056..4104
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4115..4206
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
       61 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa
      121 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
      181 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
      241 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc
      301 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg
      361 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc
      421 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac
      481 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc
      541 ctacctgaga tcaccggcga aggagggcca ccatgtacag gatgcaactc ctgtcttgca
      601 ttgcactaag tcttgcactt gtcacgaatt cgatatcggc catggttaga tctcccagag
      661 ggcccacaat caagccctgt cctccatgca aatgcccagc acctaacctc ttgggtggac
      721 catccgtctt catcttccct ccaaagatca aggatgtact catgatctcc ctgagcccca
      781 tagtcacatg tgtggtggtg gatgtgagcg aggatgaccc agatgtccag atcagctggt
      841 ttgtgaacaa cgtggaagta cacacagctc agacacaaac ccatagagag gattacaaca
      901 gtactctccg ggtggtcagt gccctcccca tccagcacca ggactggatg agtggcaagg
      961 agttcaaatg caaggtcaac aacaaagacc tcccagcgcc catcgagaga accatctcaa
     1021 aacccaaagg gtcagtaaga gctccacagg tatatgtctt gcctccacca gaagaagaga
     1081 tgactaagaa acaggtcact ctgacctgca tggtcacaga cttcatgcct gaagacattt
     1141 acgtggagtg gaccaacaac gggaaaacag agctaaacta caagaacact gaaccagtcc
     1201 tggactctga tggttcttac ttcatgtaca gcaagctgag agtggaaaag aagaactggg
     1261 tggaaagaaa tagctactcc tgttcagtgg tccacgaggg tctgcacaat caccacacga
     1321 ctaagagctt ctcccggact ccgggtaaat gagctcagct agctggccag acatgataag
     1381 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     1441 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     1501 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     1561 aagcaagtaa aacctctaca aatgtggtat ggaattaatt ctaaaataca gcatagcaaa
     1621 actttaacct ccaaatcaag cctctacttg aatccttttc tgagggatga ataaggcata
     1681 ggcatcaggg gctgttgcca atgtgcatta gctgtttgca gcctcacctt ctttcatgga
     1741 gtttaagata tagtgtattt tcccaaggtt tgaactagct cttcatttct ttatgtttta
     1801 aatgcactga cctcccacat tcccttttta gtaaaatatt cagaaataat ttaaatacat
     1861 cattgcaatg aaaataaatg ttttttatta ggcagaatcc agatgctcaa ggcccttcat
     1921 aatatccccc agtttagtag ttggacttag ggaacaaagg aacctttaat agaaattgga
     1981 cagcaagaaa gcgagcttct agcttatcct cagtcctgct cctctgccac aaagtgcacg
     2041 cagttgccgg ccgggtcgcg cagggcgaac tcccgccccc acggctgctc gccgatctcg
     2101 gtcatggccg gcccggaggc gtcccggaag ttcgtggaca cgacctccga ccactcggcg
     2161 tacagctcgt ccaggccgcg cacccacacc caggccaggg tgttgtccgg caccacctgg
     2221 tcctggaccg cgctgatgaa cagggtcacg tcgtcccgga ccacaccggc gaagtcgtcc
     2281 tccacgaagt cccgggagaa cccgagccgg tcggtccaga actcgaccgc tccggcgacg
     2341 tcgcgcgcgg tgagcaccgg aacggcactg gtcaacttgg ccatgatggc tcctcctgtc
     2401 aggagaggaa agagaagaag gttagtacaa ttgctatagt gagttgtatt atactatgca
     2461 gatatactat gccaatgatt aattgtcaaa ctagggctgc agggttcata gtgccacttt
     2521 tcctgcactg ccccatctcc tgcccaccct ttcccaggca tagacagtca gtgacttacc
     2581 aaactcacag gagggagaag gcagaagctt gagacagacc cgcgggaccg ccgaactgcg
     2641 aggggacgtg gctagggcgg cttcttttat ggtgcgccgg ccctcggagg cagggcgctc
     2701 ggggaggcct agcggccaat ctgcggtggc aggaggcggg gccgaaggcc gtgcctgacc
     2761 aatccggagc acataggagt ctcagccccc cgccccaaag caaggggaag tcacgcgcct
     2821 gtagcgccag cgtgttgtga aatgggggct tgggggggtt ggggccctga ctagtcaaaa
     2881 caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta
     2941 tccacgccca ttgatgtact gccaaaaccg catcatcatg gtaatagcga tgactaatac
     3001 gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc
     3061 gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg
     3121 tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc
     3181 tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg
     3241 cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cctgcaggtt aattaagaac
     3301 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3361 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3421 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3481 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3541 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3601 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3661 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     3721 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3781 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3841 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3901 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3961 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4021 atggctagtt aattaacatt taaatcagcg gccgcaataa aatatcttta ttttcattac
     4081 atctgtgtgt tggttttttg tgtgaatcgt aactaacata cgctctccat caaaacaaaa
     4141 cgaaacaaaa caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat
     4201 ttctctatcg aa
//
