LOCUS       V006206                 5821 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006206
VERSION     V006206
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Karig D, Martini KM, Lu T, DeLateur NA, Goldenfeld N, Weiss R.
  TITLE     Stochastic Turing patterns in a synthetic bacterial population
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (2018) In press
   PUBMED   29891706
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   Karig DK, Lu T.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2018) Research and Development Department, Johns
            Hopkins University Applied Physics Laboratory, 11100 Johns Hopkins
            Rd, Laurel, MD 20723, USA
REFERENCE   3  (bases 1 to 5821)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5821)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-MAY-2018) Research and Development Department, Johns Hopkins
            University Applied Physics Laboratory, 11100 Johns Hopkins Rd,
            Laurel, MD 20723, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(47..769)
                     /gene="rhlR"
                     /label="HTH-type quorum-sensing regulator RhlR"
                     /note="HTH-type quorum-sensing regulator RhlR from
                     Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP
                     104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
                     Accession#: P54292"
     RBS             776..787
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     regulatory      complement(779..787)
                     /label="RBSII"
                     /note="RBSII"
                     /regulatory_class="ribosome_binding_site"
     regulatory      complement(802..844)
                     /regulatory_class="promoter"
     regulatory      complement(812..817)
                     /regulatory_class="minus_10_signal"
     regulatory      complement(834..840)
                     /regulatory_class="minus_35_signal"
     protein_bind    921..937
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      946..962
                     /label="RBSH"
                     /note="RBSH"
                     /regulatory_class="ribosome_binding_site"
     CDS             963..1673
                     /label="lambda repressor"
                     /note="phage lambda repressor"
     CDS             1680..1712
                     /label="ssrA tag (LVA)"
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness
                     et al., 2006)"
     RBS             1720..1731
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     regulatory      1720..1728
                     /label="RBSII"
                     /note="RBSII"
                     /regulatory_class="ribosome_binding_site"
     CDS             1738..2451
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     terminator      2713..2799
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(2889..3968)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(3969..4046)
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     rep_origin      complement(4143..4688)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(4802..4896)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(4930..5721)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ccaaccttac cagagggcgc cccagctggc aattccgacg tcttcagatg aggcccagcg
       61 ccgcggcgta ggcggcagcc agcgtcttgt tcggcgcgtc gaacttcttc tggatgttct
      121 tgtggtggaa gttcaccgtg ctctcggaaa tgctcaggat gatggcgatt tccccggagc
      181 tcttgccgtc ggcggtccat tgcaggatct cgcgttcgcg atggctcagg cagaccgggt
      241 tggacatcag catcggatgc tccaggtcgg tcagcttctg ggtcagcaac tcgatcatgc
      301 aacgcagccg caggcgtatt tcctcgcgct cgaagctgga gatgttctgc tggtcgcgcg
      361 ccacggaaag cacgctgagc aaattgttcg gcgcgcggaa cggcaaagtc gcgccgacac
      421 agaggcccca atcgcgagcc tcgttccaga gcatccggct ctggtcgaac aggctgtcgc
      481 tccagaccac catttccgag gagcgcaggc cgttgaggat cgccggatcc acggccccgt
      541 agttctgcat ctggtatcgc tccagccagg ccttgggata ggtgccatgg acctcggtct
      601 tcggccgggt gaagggaatc gtatggcgca cgccataggc gtagtaatcg aagcccaggc
      661 gccgcacttc cttttccagg acggcgaaca cgccctggct gtcgtggatc ggctgcatct
      721 cgctacgcaa accgtcccac cacagcaaaa agcctccgtc attcctcatg cttaatttct
      781 cctctttgct ttccgggcgc tttaaccaca caatatcacc gccagaggta aaatagtcaa
      841 cacggctcgc aagtatcggc cgcccgtcct gtgaaatctg gcagttaccg ttagctttcg
      901 aattggctaa aaagtgttaa ttgtgagcgg ataacaattt actagtcaca caggacggcc
      961 ggatgagcac aaaaaagaaa ccattaacac aagagcagct tgaggacgca cgtcgcctta
     1021 aagcaattta tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct gtcgcagaca
     1081 agatggggat ggggcagtca ggcgttggtg ctttatttaa tggcatcaat gcattaaatg
     1141 cttataacgc cgcattgctt gcaaaaattc tcaaagttag cattgaagaa ttcagccctt
     1201 caatcgccag agagatctac gagatgtatg aagcggttag tatgcagccg tcacttagaa
     1261 gtgagtatga gtaccctgtt ttttctcatg ttcaggcagg gatgttctca cctgagctta
     1321 gaacctttac caaaggtgat gcggagagat gggtaagcac aaccaaaaaa gccagtgatt
     1381 ctgcattctg gcttgaggtt gaaggtaatt ccatgaccgc accaacaggc tccaagccaa
     1441 gctttcctga cggaatgtta attctcgttg accctgagca ggctgttgag cccggggatt
     1501 tctgcatagc cagacttggg ggtgatgagt ttaccttcaa gaaactgatc agggatagcg
     1561 gtcaggtgtt tttacaacca ctaaacccac agtacccaat gatcccatgc aatgagagtt
     1621 gttccgttgt ggggaaagtt atcgctagtc agtggcctga agagacgttt ggcaggcctg
     1681 cagcaaacga cgaaaactac gctttagtag cttgatcgca aagaggagaa attaagcatg
     1741 cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
     1801 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
     1861 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc
     1921 actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat
     1981 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
     2041 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
     2101 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg
     2161 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc
     2221 aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
     2281 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     2341 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
     2401 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa aaggcctgca
     2461 gcaaacgacg aaaactacgc tttagtagct taataagctt aattagctga cctactagtc
     2521 ggccgtacgg gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     2581 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     2641 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     2701 agcagtacgc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     2761 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgcccta gacctaggga
     2821 tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgcaca ttaattgcgt
     2881 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     2941 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc
     3001 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag
     3061 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg
     3121 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg
     3181 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc
     3241 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac
     3301 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat
     3361 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc
     3421 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca
     3481 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga
     3541 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg
     3601 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg
     3661 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta
     3721 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc
     3781 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc
     3841 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc
     3901 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact
     3961 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga
     4021 aaggttttgc accattcgat ggtgtcaacg tagtacaatg gcttacgaac ggggcggaga
     4081 tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg
     4141 tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgccc aaatcagtgg
     4201 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg ctcccccgtg
     4261 cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg
     4321 tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat
     4381 gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4441 caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt ggtttagagg
     4501 agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact
     4561 gcgctcctcc aagccagtta cctcggttca aagagctggt agctcagaga accttcgaaa
     4621 aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac
     4681 gatctcaaga agatcatctt attaatcaga taaaatattt ctagatttca gtgcaattta
     4741 tctcttcaaa tgtagcacct gaagtcagcc ccatacgata taagttgtta ctagtgcttg
     4801 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
     4861 gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctctcg aaccccagag
     4921 tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc
     4981 ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat
     5041 atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc
     5101 gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcacg catcgccatg
     5161 ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc
     5221 tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat
     5281 ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg
     5341 atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc
     5401 aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc
     5461 cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga
     5521 tagccgcgct gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa
     5581 aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt
     5641 ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg
     5701 caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cagatcttga
     5761 tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt
     5821 c
//
