LOCUS       pFN6A_(HQ).        3471 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Flexi(R) vector with an ampicillin resistance marker, for bacterial 
            or in vitro expression of a protein with an N-terminal HQ metal 
            affinity tag.
ACCESSION   .
VERSION     .
KEYWORDS    pFN6A (HQ)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3471)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Subclone a coding sequence between the SgfI/AsiSI and PmeI sites to 
            remove the lethal barnase gene.
FEATURES             Location/Qualifiers
     source          1..3471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..39
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             70..72
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             76..93
                     /label=HQ tag
                     /note="HQHQHQ metal affinity tag"
     CDS             125..457
                     /label=barnase
                     /note="ribonuclease from Bacillus amyloliquefaciens"
     misc_feature    470..525
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      589..636
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        865..969
                     /label=AmpR promoter
     CDS             970..1827
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      complement(1988..2576)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2692..2975
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to
                     maintain plasmids as monomers"
     terminator      3205..3291
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3383..3410
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
ORIGIN
        1 ggatctcgat cccgcgaaat taatacgact cactataggc aattttaact ttactaagga
       61 gaattcacca tgaaacatca acatcagcac caagcgatcg ccatggaata agtaaggaat
      121 ccacatggca caggttatca acacgtttga cggggttgcg gattatcttc agacatatca
      181 taagctacct gataattaca ttacaaaatc agaagcacaa gccctcggct gggtggcatc
      241 aaaagggaac cttgcagacg tcgctccggg gaaaagcatc ggcggagaca tcttctcaaa
      301 cagggaaggc aaactcccgg gcaaaagcgg acgaacatgg cgtgaagcgg atattaacta
      361 tacatcaggc ttcagaaatt cagaccggat tctttactca agcgactggc tgatttacaa
      421 aacaacggac cattatcaga cctttacaaa aatcagataa tgtttaaacg aattcgggct
      481 cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagctgatcc ggctgctaac
      541 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
      601 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccggt
      661 tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct
      721 acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc
      781 atccggggtc agcaccgttt ctgcggactg gctttctacg taatggtttc ttagacgtca
      841 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      901 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
      961 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     1021 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     1081 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     1141 tttcgccccg aagaacgttt tccaatgatg agcactttca aagttctgct atgtggcgcg
     1201 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     1261 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     1321 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     1381 acaactatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     1441 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     1501 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     1561 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     1621 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     1681 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     1741 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     1801 ataggtgcct cactgattaa gcattggtaa ttcgaaatga ccgaccaagc gacgcccaac
     1861 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     1921 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1981 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     2041 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     2101 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     2161 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     2221 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     2281 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     2341 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     2401 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     2461 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2521 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tttcaagaag
     2581 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     2641 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttatag tccggaaata
     2701 caggaacgca cgctggatgg cccttcgctg ggatggtgaa accatgaaaa atggcagctt
     2761 cagtggatta agtgggggta atgtggcctg taccctctgg ttgcataggt attcatacgg
     2821 ttaaaattta tcaggcgcga ttgcggcagt ttttcgggtg gtttgttgcc atttttacct
     2881 gtctgctgcc gtgatcgcgc tgaacgcgtt ttagcggtgc gtacaattaa gggattatgg
     2941 taaatccact tactgtctgc cctcgtagcc atcgagataa accgcagtac tccggccacg
     3001 atgcgtccgg cgtagaggat cgagatcttt tcagcctgat acagattaaa tcagaacgca
     3061 gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc
     3121 ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg
     3181 cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     3241 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga
     3301 gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa
     3361 actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta
     3421 caaactcttt tgtttatttt tctaaataca ttcaaatatg tatccgctca t
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