LOCUS       40924_20226        9297 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Site-specific excision vector pFLP2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9297)
  AUTHORS   Hoang TT, Karkhoff-Schweizer RR, Kutchma AJ, Schweizer HP.
  TITLE     A broad-host-range Flp-FRT recombination system for site-specific 
            excision of chromosomally-located DNA sequences: application for 
            isolation of unmarked Pseudomonas aeruginosa mutants
  JOURNAL   Gene 212 (1), 77-86 (1998)
  PUBMED    9661666
REFERENCE   2  (bases 1 to 9297)
  AUTHORS   Hoang TT, Karkhoff-Schweizer RR, Kutchma AJ, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-FEB-1998) Microbiology, Colorado State University, 
            Fort Collins, CO 80523, USA
REFERENCE   3  (bases 1 to 9297)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9297)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1998"; volume: "212"; issue: "1"; pages: "77-86"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-FEB-1998) Microbiology, Colorado State University, Fort Collins,
            CO 80523, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(232..583)
                     /direction=LEFT
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             597..1427
                     /codon_start=1
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
                     /translation="VASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     primer_bind     1591..1607
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2341..3759)
                     /codon_start=1
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     promoter        complement(3760..4205)
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             complement(4429..5697)
                     /codon_start=1
                     /label=FLP
                     /note="site-specific recombinase"
                     /translation="MPQFGILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     RBS             5701..5709
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             5798..6508
                     /codon_start=1
                     /label=lambda repressor (ts)
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     primer_bind     complement(6800..6816)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6824..6840)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6848..6878)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6893..6914)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     oriT            7298..7406
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      complement(7478..8066)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8240..9097)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9098..9202)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatacctc tcaggcgccg ctggtgccgc tggttggacg ccaagggtga atccgcctcg
      241 ataccctgat tactcgcttc ctgcgccctc tcaggcggcg ataggggact ggtaaaacgg
      301 ggattgccca gacgcctccc ccgccccttc aggggcacaa atgcggcccc aacggggcca
      361 cgtagtggtg cgttttttgc gtttccaccc ttttcttcct tttccctttt aaacctttta
      421 ggacgtctac aggccacgta atccgtggcc tgtagagttt aaaaagggac ggatttgttg
      481 ccattaaggg acggatttgt tgttaagaag ggacggattt gttgttgtaa agggacggat
      541 ttgttgtatt gtgggacgca gatacagtgt ccccttatac acaaggaatg tcgaacgtgg
      601 cctcaccccc aatggtttac aaaagcaatg ccctggtcga ggccgcgtat cgcctcagtg
      661 ttcaggaaca gcggatcgtt ctggcctgta ttagccaggt gaagaggagc gagcctgtca
      721 ccgatgaagt gatgtattca gtgacggcgg aggacatagc gacgatggcg ggtgtcccta
      781 tcgaatcttc ctacaaccag ctcaaagaag cggccctgcg cctgaaacgg cgggaagtcc
      841 ggttaaccca agagcccaat ggcaagggga aaagaccgag tgtgatgatt accggctggg
      901 tgcaaacaat catctaccgg gagggtgagg gccgtgtaga actcaggttc accaaagaca
      961 tgctgccgta cctgacggaa ctcaccaaac agttcaccaa atacgccttg gctgacgtgg
     1021 ccaagatgga cagcacccac gcgatcaggc tttacgagct gctcatgcaa tgggacagca
     1081 tcggccagcg cgaaatagaa attgaccagc tgcgaaagtg gtttcaactg gaaggccggt
     1141 atccctcgat caaggacttc aagttgcgag tgcttgatcc agccgtgacg cagatcaacg
     1201 agcacagccc gctacaggtg gagtgggcgc agcgaaagac cgggcgcaag gtcacacatc
     1261 tgttgttcag ttttggaccg aagaagcccg ccaaggcggt gggtaaggcc ccagcgaagc
     1321 gcaaggccgg gaagatttca gatgctgaga tcgcgaaaca ggctcgccct ggtgagacat
     1381 gggaagcggc ccgcgctcga ctaacccaga tgccgctgga tctggcctag aggccgtggc
     1441 caccacggcc cggcctgcct ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1501 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1561 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat
     1621 gcctgcagtt cacttacacc gcttctcaac ccggtacgca ccagaaaatc attgatatgg
     1681 ccatgaatgg cgttggatgc cgggcaacag cccgcattat gggcgttggc ctcaacacga
     1741 ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac agccgggcag
     1801 tgacgtcatc gtctgcgcgg aaatggacga acagtggggc tatgtcgggg ctaaatcgcg
     1861 ccagcgctgg ctgttttacg cgtatgacag tctccggaag acggttgttg cgcacgtatt
     1921 cggtgaacgc actatggcga cgctggggcg tcttatgagc ctgctgtcac cctttgacgt
     1981 ggtgatatgg atgacggatg gctggccgct gtatgaatcc cgcctgaagg gaaagctgca
     2041 cgtaatcagc aagcgatata cgcagcgaat tgagcggcat aacctgaatc tgaggcagca
     2101 cctggcacgg ctgggacgga agtcgctgtc gttctcaaaa tcggtggagc tgcatgacaa
     2161 agtcatcggg cattatctga acataaaaca ctatcaataa gttggagtca ttaccaaaag
     2221 gttaggaata cggttagcca tttgcctgct tttatatagt tcatatggga ttcaccttta
     2281 tgttgataag aaataaaaga aaatgccaat aggatatcgg cattttcttt tgcgttttta
     2341 tttgttaact gttaattgtc cttgttcaag gatgctgtct ttgacaacag atgttttctt
     2401 gcctttgatg ttcagcagga agctaggcgc aaacgttgat tgtttgtctg cgtagaatcc
     2461 tctgtttgtc atatagcttg taatcacgac attgtttcct ttcgcttgag gtacagcgaa
     2521 gtgtgagtaa gtaaaggtta catcgttagg atcaagatcc atttttaaca caaggccagt
     2581 tttgttcagc ggcttgtatg ggccagttaa agaattagaa acataaccaa gcatgtaaat
     2641 atcgttagac gtaatgccgt caatcgtcat ttttgatccg cgggagtcag tgaacagata
     2701 ccatttgccg ttcattttaa agacgttcgc gcgttcaatt tcatctgtta ctgtgttaga
     2761 tgcaatcagc ggtttcatca cttttttcag tgtgtaatca tcgtttagct caatcatacc
     2821 gagagcgccg tttgctaact cagccgtgcg ttttttatcg ctttgcagaa gtttttgact
     2881 ttcttgacgg aagaatgatg tgcttttgcc atagtatgct ttgttaaata aagattcttc
     2941 gccttggtag ccatcttcag ttccagtgtt tgcttcaaat actaagtatt tgtggccttt
     3001 atcttctacg tagtgaggat ctctcagcgt atggttgtcg cctgagctgt agttgccttc
     3061 atcgatgaac tgctgtacat tttgatacgt ttttccgtca ccgtcaaaga ttgatttata
     3121 atcctctaca ccgttgatgt tcaaagagct gtctgatgct gatacgttaa cttgtgcagt
     3181 tgtcagtgtt tgtttgccgt aatgtttacc ggagaaatca gtgtagaata aacggatttt
     3241 tccgtcagat gtaaatgtgg ctgaacctga ccattcttgt gtttggtctt ttaggataga
     3301 atcatttgca tcgaatttgt cgctgtcttt aaagacgcgg ccagcgtttt tccagctgtc
     3361 aatagaagtt tcgccgactt tttgatagaa catgtaaatc gatgtgtcat ccgcattttt
     3421 aggatctccg gctaatgcaa agacgatgtg gtagccgtga tagtttgcga cagtgccgtc
     3481 agcgttttgt aatggccagc tgtcccaaac gtccaggcct tttgcagaag agatattttt
     3541 aattgtggac gaatcgaact caggaacttg atatttttca tttttttgct gttcagggat
     3601 ttgcagcata tcatggcgtg taatatggga aatgccgtat gtttccttat atggcttttg
     3661 gttcgtttct ttcgcaaacg cttgagttgc gcctcctgcc agcagtgcgg tagtaaaggt
     3721 taatactgtt gcttgttttg caaacttttt gatgttcatc gttcatgtct ccttttttat
     3781 gtactgtgtt agcggtctgc ttcttccagc cctcctgttt gaagatggca agttagttac
     3841 gcacaataaa aaaagaccta aaatatgtaa ggggtgacgc caaagtatac actttgccct
     3901 ttacacattt taggtcttgc ctgctttatc agtaacaaac ccgcgcgatt tacttttcga
     3961 cctcattcta ttagactctc gtttggattg caactggtct attttcctct tttgtttgat
     4021 agaaaatcat aaaaggattt gcagactacg ggcctaaaga actaaaaaat ctatctgttt
     4081 cttttcattc tctgtatttt ttatagtttc tgttgcatgg gcataaagtt gcctttttaa
     4141 tcacaattca gaaaatatca taatatctca tttcactaaa taatagtgaa cggcaggtat
     4201 atgtgatggg ttaaaaagga tcgatcctct agaggatccc cctagagaat aggaacttcg
     4261 gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc
     4321 gcacatacag ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat
     4381 acatgagaag aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt
     4441 tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta
     4501 tcgtatgctt ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg
     4561 attagtctca tccttcaatg ctatcatttc ctttgatatt ggatcatatg catagtaccg
     4621 agaaactagt gcgaagtagt gatcaggtat tgctgttatc tgatgagtat acgttgtcct
     4681 ggccacggca gaagcacgct tatcgctcca atttcccaca acattagtca actccgttag
     4741 gcccttcatt gaaagaaatg aggtcatcaa atgtcttcca atgtgagatt ttgggccatt
     4801 ttttatagca aagattgaat aaggcgcatt tttcttcaaa gctttattgt acgatctgac
     4861 taagttatct tttaataatt ggtattcctg tttattgctt gaagaattgc cggtcctatt
     4921 tactcgtttt aggactggtt cagaattcct caaaaattca tccaaatata caagtggatc
     4981 gatcctaccc cttgcgctaa agaagtatat gtgcctacta acgcttgtct ttgtctctgt
     5041 cactaaacac tggattatta ctcccagata cttattttgg actaatttaa atgatttcgg
     5101 atcaacgttc ttaatatcgc tgaatcttcc acaattgatg aaagtagcta ggaagaggaa
     5161 ttggtataaa gtttttgttt ttgtaaatct cgaagtatac tcaaacgaat ttagtatttt
     5221 ctcagtgatc tcccagatgc tttcaccctc acttagaagt gctttaagca tttttttact
     5281 gtggctattt cccttatctg cttcttccga tgattcgaac tgtaattgca aactacttac
     5341 aatatcagtg atatcagatt gatgtttttg tccatagtaa ggaataattg taaattccca
     5401 agcaggaatc aatttcttta atgaggcttc cagaattgtt gctttttgcg tcttgtattt
     5461 aaactggagt gatttattga caatatcgaa actcagcgaa ttgcttatga tagtattata
     5521 gctcatgaat gtggctctct tgattgctgt tccgttatgt gtaatcatcc aacataaata
     5581 ggttagttca gcagcacata atgctatttt ctcacctgaa ggtctttcaa acctttccac
     5641 aaactgacga acaagcacct taggtggtgt tttacataat ataccaaatt gtggcataca
     5701 acctccttag tacatgcaac cattatcacc gccagaggta aaatagtcaa cacgcacggt
     5761 gttagatatt tatcccttgc ggtgatagat ttaacgtatg agcacaaaaa agaaaccatt
     5821 aacacaagag cagcttgagg acgcacgtcg ccttaaagca atttatgaaa aaaagaaaaa
     5881 tgaacttggc ttatcccagg aatctgtcgc agacaagatg gggatggggc agtcaggcgt
     5941 tggtgcttta tttaatggca tcaatgcatt aaatgcttat aacgccgcat tgcttacaaa
     6001 aattctcaaa gttagcgttg aagaatttag cccttcaatc gccagagaaa tctacgagat
     6061 gtatgaagcg gttagtatgc agccgtcact tagaagtgag tatgagtacc ctgttttttc
     6121 tcatgttcag gcagggatgt tctcacctaa gcttagaacc tttaccaaag gtgatgcgga
     6181 gagatgggta agcacaacca aaaaagccag tgattctgca ttctggcttg aggttgaagg
     6241 taattccatg accgcaccaa caggctccaa gccaagcttt cctgacggaa tgttaattct
     6301 cgttgaccct gagcaggctg ttgagccagg tgatttctgc atagccagac ttgggggtga
     6361 tgagtttacc ttcaagaaac tgatcaggga tagcggtcag gtgtttttac aaccactaaa
     6421 cccacagtac ccaatgatcc catgcaatga gagttgttcc gttgtgggga aagttatcgc
     6481 tagtcagtgg cctgaagaga cgtttggctg atcggcaagg tgttctggtc ggcgcatagc
     6541 tgataacaat tgagcaagaa tcttcatcga attaggggaa ttttcactcc cctcagaaca
     6601 taacatagta aatggattga attatgaaga atggttttta tgcgacttac cgcagcaaaa
     6661 ataaagggaa agataagcgc tcaataaacc tgtctgtttt ccttaattct ctgctggctg
     6721 ataatcatca cctgcaggtt ggctccaatt atttgtatat tcataaaatc ggggtaccga
     6781 gctcgaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6841 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6901 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6961 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     7021 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     7081 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7141 acatggggat tccttaaggt atactttccg ctgcataacc ctgcttcggg gtcattatag
     7201 cgattttttc ggtatatcca tcctttttcg cacgatatac aggattttgc caaagggttc
     7261 gtgtagactt tccttggtgt atccaacggc gtcagccggg caggataggt gaagtaggcc
     7321 cacccgcgag cgggtgttcc ttcttcactg tcccttattc gcacctggcg gtgctcaacg
     7381 ggaatcctgc tctgcgaggc tggccgataa gctagcttat gtgagcaaaa ggccagcaaa
     7441 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7501 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7561 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7621 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7681 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7741 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7801 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7861 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     7921 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7981 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8041 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     8101 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8161 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8221 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8281 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8341 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8401 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8461 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8521 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     8581 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8641 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8701 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8761 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8821 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     8881 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8941 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     9001 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     9061 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     9121 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9181 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     9241 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc
//
