LOCUS       40924_19541        3704 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid for yeast gene deletion using the natMX6 selectable marker 
            conferring nourseothricin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Hentges P, Van Driessche B, Tafforeau L, Vandenhaute J, Carr AM.
  TITLE     Three novel antibiotic marker cassettes for gene disruption and 
            marker switching in Schizosaccharomyces pombe.
  JOURNAL   Yeast 2005;22:1013-9.
  PUBMED    16200533
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   EUROSCARF
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3704)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2005"; volume: "22"; pages: "1013-9"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            113..1235
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     promoter        complement(1340..1358)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1616..2204)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2378..3235)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3236..3340)
                     /label=AmpR promoter
     promoter        3686..3704
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 acgcggccgc cagctgaagc ttcgtacgct gcaggtcgac ggatccccgg gttaattaag
       61 gcgcgccaga tctgtttagc ttgcctcgtc cccgccgggt cacccggcca gcgacatgga
      121 ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg
      181 tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca tacattttga
      241 tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg cagacctgcg agcagggaaa
      301 cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa
      361 aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg
      421 atacagttct cacatcacat ccgaacataa acaaccatgg gtaccactct tgacgacacg
      481 gcttaccggt accgcaccag tgtcccgggg gacgccgagg ccatcgaggc actggatggg
      541 tccttcacca ccgacaccgt cttccgcgtc accgccaccg gggacggctt caccctgcgg
      601 gaggtgccgg tggacccgcc cctgaccaag gtgttccccg acgacgaatc ggacgacgaa
      661 tcggacgacg gggaggacgg cgacccggac tcccggacgt tcgtcgcgta cggggacgac
      721 ggcgacctgg cgggcttcgt ggtcatctcg tactcggcgt ggaaccgccg gctgaccgtc
      781 gaggacatcg aggtcgcccc ggagcaccgg gggcacgggg tcgggcgcgc gttgatgggg
      841 ctcgcgacgg agttcgccgg cgagcggggc gccgggcacc tctggctgga ggtcaccaac
      901 gtcaacgcac cggcgatcca cgcgtaccgg cggatggggt tcaccctctg cggcctggac
      961 accgccctgt acgacggcac cgcctcggac ggcgagcggc aggcgctcta catgagcatg
     1021 ccctgcccct aatcagtact gacaataaaa agattcttgt tttcaagaac ttgtcatttg
     1081 tatagttttt ttatattgta gttgttctat tttaatcaaa tgttagcgtg atttatattt
     1141 tttttcgcct cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa gtaatatcat
     1201 gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc gattcgatac taacgccgcc
     1261 atccagttta aacgagctcg aattcatcga tgatatcaga tccactagtg gcctatgcgg
     1321 ccgcggatct gccggtctcc ctatagtgag tcgtattaat ttcgataagc caggttaacc
     1381 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     1441 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     1501 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     1561 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1621 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1681 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1741 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1801 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     1861 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1921 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     1981 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2041 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2101 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2161 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2221 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     2281 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     2341 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     2401 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     2461 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     2521 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     2581 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     2641 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     2701 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     2761 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     2821 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     2881 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     2941 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     3001 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     3061 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     3121 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     3181 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     3241 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     3301 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     3361 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     3421 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     3481 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     3541 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     3601 ttgtactgag agtgcaccat atggacatat tgtcgttaga acgcggctac aattaataca
     3661 taaccttatg tatcatacac atacgattta ggtgacacta taga
//
