LOCUS       40924_19651        4705 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast tagging vector pFA6a-VN-kanMX6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Sung MK, Huh WK.
  TITLE     Bimolecular fluorescence complementation analysis system for in vivo
            detection of protein-protein interaction in Saccharomyces cerevisiae
  JOURNAL   Yeast 24 (9), 767-775 (2007)
  PUBMED    17534848
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Sung M-K., Huh W-K.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2007) School of Biological Sciences, Seoul 
            National University, Seoul 151-747, Republic of Korea
REFERENCE   3  (bases 1 to 4705)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4705)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2007"; volume: "24"; issue: "9"; pages: "767-775"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-JAN-2007) School of Biological Sciences, Seoul National 
            University, Seoul 151-747, Republic of Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             78..596
                     /codon_start=1
                     /label=VN173
                     /note="N-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     terminator      620..807
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     gene            882..2238
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     promoter        complement(2343..2361)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2619..3207)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3381..4238)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4239..4343)
                     /label=AmpR promoter
     promoter        4689..4705
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 acagatccat cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      121 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      181 agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc
      241 ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct
      301 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      361 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      421 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      481 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg
      541 ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgagtagg
      601 gcgcgccact tctaaataag cgaatttctt atgatttatg atttttatta ttaaataagt
      661 tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt
      721 cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat agtatgaggt
      781 cgctcttatt gaccacacct ctaccggcag atccgctagg gataacaggg taatatagat
      841 ctgtttagct tgcctcgtcc ccgccgggtc acccggccag cgacatggag gcccagaata
      901 ccctccttga cagtcttgac gtgcgcagct caggggcatg atgtgactgt cgcccgtaca
      961 tttagcccat acatccccat gtataatcat ttgcatccat acattttgat ggccgcacgg
     1021 cgcgaagcaa aaattacggc tcctcgctgc agacctgcga gcagggaaac gctcccctca
     1081 cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag aaatataaaa ggttaggatt
     1141 tgccactgag gttcttcttt catatacttc cttttaaaat cttgctagga tacagttctc
     1201 acatcacatc cgaacataaa caaccatggg taaggaaaag actcacgttt cgaggccgcg
     1261 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     1321 gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct
     1381 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     1441 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     1501 atggttactc accactgcga tccccggcaa aacagcattc caggtattag aagaatatcc
     1561 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     1621 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
     1681 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     1741 tgttgaacaa gtctggaaag aaatgcataa gcttttgcca ttctcaccgg attcagtcgt
     1801 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     1861 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     1921 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     1981 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcagt
     2041 actgacaata aaaagattct tgttttcaag aacttgtcat ttgtatagtt tttttatatt
     2101 gtagttgttc tattttaatc aaatgttagc gtgatttata ttttttttcg cctcgacatc
     2161 atctgcccag atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga
     2221 atgctggtcg ctatactgct gtcgattcga tactaacgcc gccatccagt ttaaacgagc
     2281 tcgaattcat cgatgatatc agatccacta gtggcctatg cggccgcgga tctgccggtc
     2341 tccctatagt gagtcgtatt aatttcgata agccaggtta acctgcatta atgaatcggc
     2401 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     2461 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     2521 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     2581 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     2641 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2701 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2761 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca
     2821 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     2881 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2941 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3001 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     3061 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3121 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3181 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3241 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     3301 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     3361 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     3421 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     3481 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     3541 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     3601 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     3661 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     3721 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     3781 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     3841 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     3901 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     3961 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     4021 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     4081 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     4141 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     4201 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     4261 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     4321 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     4381 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     4441 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     4501 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     4561 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     4621 catatggaca tattgtcgtt agaacgcggc tacaattaat acataacctt atgtatcata
     4681 cacatacgat ttaggtgaca ctata
//
