LOCUS       40924_19561        5004 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast tagging vector pFA6a-TAP-KlURA3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Sung M-K., Huh W-K.
  TITLE     Yeast tagging vector pFA6a-TAP-KlURA3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Sung M-K., Huh W-K.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2007) School of Biological Sciences, Seoul 
            National University, Seoul 151-747, Republic of Korea
REFERENCE   3  (bases 1 to 5004)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5004)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-OCT-2007) School of Biological Sciences, Seoul National 
            University, Seoul 151-747, Republic of Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             69..146
                     /codon_start=1
                     /label=CBP
                     /note="calmodulin-binding peptide"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             174..194
                     /codon_start=1
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
                     /translation="ENLYFQG"
     CDS             225..398
                     /codon_start=1
                     /label=ProtA
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             402..572
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="DNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLA
                     EAKKLNGAQAPK"
     CDS             1241..2044
                     /codon_start=1
                     /gene="URA3"
                     /product="orotidine 5'-phosphate decarboxylase"
                     /label=URA3
                     /protein_id="ABX47171.1"
                     /translation="MSTKSYTSRAETHASPVASKLLRLMDEKKTNLCASLDVRSTDELL
                     KLVETLGPYICLLKTHVDILDDFSYEGTVVPLKALAEKYKFLIFEDRKFADIGNTVKLQ
                     YTSGVYRIAEWSDITNAHGVTGAGIVAGLKQGAQEVTKEPRGLLMLAELSSKGSLAHGE
                     YTKGTVDIAKSDKDFVIGFIAQNDMGGREEGFDWLIMTPGVGLDDKGDALGQQYRTVDE
                     VVSGGSDIIIVGRGLFAKGRDPKVEGERYRNAGWEAYQKRISAPH"
     gene            1241..2044
                     /gene="URA3"
                     /label=URA3
     terminator      2340..2537
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(2642..2660)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2918..3506)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3680..4537)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4538..4642)
                     /label=AmpR promoter
     promoter        4988..5004
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 atccatggaa gagaagatgg aaaaagaatt tcatagccgt ctcagcagcc aaccgcttta
      121 agaaaatctc atcctccggg gcacttgatt atgatattcc aactactgct agcgagaatt
      181 tgtattttca gggagaattc ggccttgcgc aacacgatga agccgtggac aacaaattca
      241 acaaagaaca acaaaacgcg ttctatgaga tcttacattt acctaactta aacgaagaac
      301 aacgaaacgc cttcatccaa agtttaaaag atgacccaag ccaaagcgct aaccttttag
      361 cagaagctaa aaagctaaat gatgctcagg cgccgaaagt agacaacaaa ttcaacaaag
      421 aacaacaaaa cgcgttctat gagatcttac atttacctaa cttaaacgaa gaacaacgaa
      481 acgccttcat ccaaagttta aaagatgacc caagccaaag cgctaacctt ttagcagaag
      541 ctaaaaagct aaatggtgct caggcgccga aagtagacgc gaattccgcg gggaagtcaa
      601 cctgaggcgc gcctctctgc ttttgtgcgc gtatgtttat gtatgtacct ctctctctat
      661 ttctattttt aaaccaccct ctcaataaaa taaaaataat aaagtatttt taaggaaaag
      721 acgtgtttaa gcactgactt tatctacttt ttgtacgttt tcattgatat aatgtgtttt
      781 gtctctccct tttctacgaa aatttcaaaa attgaccaaa aaaaggaata tatatacgaa
      841 aaactattat atttatatat catagtgttg ataaaaaatg tttatcagat ctcggagaca
      901 atcatatggg agaagcaatt ggaagataga aaaaaggtac tcggtacata aatatatgtg
      961 attctgggta gaagatcggt ctgcattgga tggtggtaac gcattttttt acacacatta
     1021 cttgcctcga gcatcaaatg gtggttattc gtggatctat atcacgtgat ttgcttaaga
     1081 attgtcgttc atggtgacac ttttagcttt gacatgatta agctcatctc aattgatgtt
     1141 atctaaagtc atttcaacta tctaagatgt ggttgtgatt gggccatttt gtgaaagcca
     1201 gtacgccagc gtcaatacac tcccgtcaat tagttgcacc atgtccacaa aatcatatac
     1261 cagtagagct gagactcatg caagtccggt tgcatcgaaa cttttacgtt taatggatga
     1321 aaagaagacc aatttgtgtg cttctcttga cgttcgttcg actgatgagc tattgaaact
     1381 tgttgaaacg ttgggtccat acatttgcct tttgaaaaca cacgttgata tcttggatga
     1441 tttcagttat gagggtactg tcgttccatt gaaagcattg gcagagaaat acaagttctt
     1501 gatatttgag gacagaaaat tcgccgatat cggtaacaca gtcaaattac aatatacatc
     1561 gggcgtttac cgtatcgcag aatggtctga tatcaccaac gcccacgggg ttactggtgc
     1621 tggtattgtt gctggcttga aacaaggtgc gcaagaggtc accaaagaac caaggggatt
     1681 attgatgctt gctgaattgt cttccaaggg ttctctagca cacggtgaat atactaaggg
     1741 taccgttgat attgcaaaga gtgataaaga tttcgttatt gggttcattg ctcagaacga
     1801 tatgggagga agagaagaag ggtttgattg gctaatcatg accccaggtg taggtttaga
     1861 cgacaaaggc gatgcattgg gtcagcagta cagaaccgtc gacgaagttg taagtggtgg
     1921 atcagatatc atcattgttg gcagaggact tttcgccaag ggtagagatc ctaaggttga
     1981 aggtgaaaga tacagaaatg ctggatggga agcgtaccaa aagagaatca gcgctcccca
     2041 ttaattatac aggaaactta atagaacaaa tcacatattt aatctaatag ccacctgcat
     2101 tggcacggtg caacactcac ttcaacttca tcttacaaaa gatcacgtga tctgttgtat
     2161 tgaactgaaa attttttgtt tgcttctctc tctctctttc attatgtgag agtttaaaaa
     2221 ccagaaacta catcatcgaa aaagagttta aaccattaca accattgcga taagccctct
     2281 caaacttcct ccagaaccaa tgacgttccc tcaaccaaag gtgttttgat gtgaagagta
     2341 ctgacaataa aaagattctt gttttcaaga acttgtcatt tgtatagttt ttttatattg
     2401 tagttgttct attttaatca aatgttagcg tgatttatat tttttttcgc ctcgacatca
     2461 tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa
     2521 tgctggtcgc tatactgctg tcgattcgat actaacgccg ccatccagtt taaacgagct
     2581 cgaattcatc gatgatatca gatccactag tggcctatgc ggccgcggat ctgccggtct
     2641 ccctatagtg agtcgtatta atttcgataa gccaggttaa cctgcattaa tgaatcggcc
     2701 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     2761 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     2821 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2881 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2941 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     3001 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     3061 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
     3121 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3181 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3241 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     3301 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     3361 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3421 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3481 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     3541 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     3601 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3661 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     3721 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     3781 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     3841 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3901 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3961 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     4021 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     4081 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     4141 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     4201 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     4261 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     4321 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     4381 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     4441 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     4501 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     4561 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     4621 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     4681 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     4741 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     4801 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     4861 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     4921 atatggacat attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac
     4981 acatacgatt taggtgacac tata
//
