LOCUS V029255 5367 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V029255 VERSION V029255 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..1514 /codon_start=1 /note="This allele of Thy1 is also known as CD90.2." /product="mouse GPI-anchored cell surface glycoprotein of the immunoglobulin superfamily" /transl_table=1 /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF CELQVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL" /label="Thy1.2" CDS 1533..1562 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 1581..1604 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(1624..1640) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 1651..2273 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(2422..3010) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 3339..3386 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(3618..4412) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(4447..4804) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(4806..4910) /gene="bla" /label="AmpR promoter" rep_origin 4937..5365 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat gaacccagcc atcagcgtcg ctctcctgct ctcagtcttg caggtgtccc 1081 gagggcagaa ggtgaccagc ctgacagcct gcctggtgaa ccaaaacctt cgcctggact 1141 gccgccatga gaataacacc aaggataact ccatccagca tgagttcagc ctgacccgag 1201 agaagaggaa gcacgtgctc tcaggcaccc ttgggatacc cgagcacacg taccgctccc 1261 gcgtcaccct ctccaaccag ccctatatca aggtccttac cctagccaac ttcaccacca 1321 aggatgaggg cgactacttt tgtgagcttc aagtctcggg cgcgaatccc atgagctcca 1381 ataaaagtat cagtgtgtat agagacaagc tggtcaagtg tggcggcata agcctgctgg 1441 ttcagaacac atcctggatg ctgctgctgc tgctttccct ctccctcctc caagccctgg 1501 acttcatttc tctgacgcgt acgcggccgc tcgagcagaa actcatctca gaagaggatc 1561 tggcagcaaa tgatatcctg gattacaagg atgacgacga taaggtttaa acggccggcc 1621 gcggtcatag ctgtttcctg aacagatccc gggtggcatc cctgtgaccc ctccccagtg 1681 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta 1741 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg 1801 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa 1861 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa 1921 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc 1981 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc 2041 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg 2101 tgaaccactg ctcccttccc tgtccttctg attttaaaat aactatacca gcaggaggac 2161 gtccagacac agcataggct acctggccat gcccaaccgg tgggacattt gagttgcttg 2221 cttggcactg tcctctcatg cgttgggtcc actcagtaga tgcctgttga attgggtacg 2281 cggccagcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 2341 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 2401 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 2461 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 2521 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 2581 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 2641 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 2701 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 2761 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 2821 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 2881 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 2941 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 3001 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 3061 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 3121 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaacctg aggctatggc 3181 agggcctgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttggggggtg 3241 gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg gtggggtatc 3301 gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc caacaccgtg 3361 cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt 3421 ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac tccagcatga gatccccgcg 3481 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa 3541 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc 3601 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc 3661 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc 3721 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcgatccgc cacacccagc 3781 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag 3841 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg 3901 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga 3961 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg 4021 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc 4081 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc 4141 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg 4201 gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg 4261 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag 4321 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga 4381 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga 4441 tcgatctttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc 4501 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg 4561 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat 4621 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga 4681 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg 4741 ggagcctggg gactttccac accctaactg acacacattc cacagctggt tctttccgcc 4801 tcaggactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 4861 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 4921 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 4981 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 5041 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 5101 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 5161 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 5221 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 5281 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 5341 atttaacaaa aatttaacgc gaatttt //