LOCUS V019511 7298 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V019511 VERSION V019511 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 683..1954 /label="STAMBP(NM_213622)" /note="STAMBP(NM_213622)" /gene="STAMBP" CDS 1961..2050 /codon_start=1 /product="3 tandem Myc epitope tags" /transl_table=1 /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" /label="3xMyc" misc_feature 2074..2662 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 2728..2776 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 2978..3406 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3420..3749 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3816..4610 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4786..4907 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4956..4972) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4980..4996 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5004..5034) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5049..5070 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5358..5946) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6117..6977) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6978..7082) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccgcca ccatgtctga ccatggagat gtgagcctcc cgcccgaaga 721 ccgggtgagg gctctctccc agctgggtag tgcggtagag gtgaatgaag acattccacc 781 ccgtcggtac ttccgctctg gagttgagat tatccgaatg gcatccattt actctgagga 841 aggcaacatt gaacatgcct tcatcctcta taacaagtat atcacgctct ttattgagaa 901 actaccaaaa catcgagatt acaaatctgc tgtcattcct gaaaagaaag acacagtaaa 961 gaaattaaag gagattgcat ttcccaaagc agaagagctg aaggcagagc tgttaaaacg 1021 atataccaaa gaatatacag aatataatga agaaaagaag aaggaagcag aggaattggc 1081 ccggaacatg gccatccagc aagagctgga aaaggaaaaa cagagggtag cacaacagaa 1141 gcagcagcaa ttggaacagg aacagttcca tgccttcgag gagatgatcc ggaaccagga 1201 gctagaaaaa gagcgactga aaattgtaca ggagtttggg aaggtagacc ctggcctagg 1261 tggcccgcta gtgcctgact tggagaagcc ctccttagat gtgttcccca ccttaacagt 1321 ctcatccata cagccttcag actgtcacac aactgtaagg ccagctaagc cacctgtggt 1381 ggacaggtcc ttgaaacctg gagcactgag caactcagaa agtattccca caatcgatgg 1441 attgcgccat gtggtggtgc ctgggcggct gtgcccacag tttctccagt tagccagtgc 1501 caacactgcc cggggagtgg agacatgtgg aattctctgt ggaaaactga tgaggaatga 1561 atttaccatt acccatgttc tcatccccaa gcaaagtgct gggtctgatt actgcaacac 1621 agagaacgaa gaagaacttt tcctcataca ggatcagcag ggcctcatca cactgggctg 1681 gattcatact caccccacac agaccgcgtt tctctccagt gtcgacctac acactcactg 1741 ctcttaccag atgatgttgc cagagtcagt agccattgtt tgctccccca agttccagga 1801 aactggattc tttaaactaa ctgaccatgg actagaggag atttcttcct gtcgccagaa 1861 aggatttcat ccacacagca aggatccacc tctgttctgt agctgcagcc acgtgactgt 1921 tgtggacaga gcagtgacca tcacagacct tcgactcgag gaacaaaaac taatatcaga 1981 ggaagatttg gaacagaaac taatcagtga agaagattta gagcaaaagt taatttccga 2041 ggaggactta tagtgagggc ccgatatctc gacaatcaac ctctggatta caaaatttgt 2101 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 2161 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 2221 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 2281 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 2341 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 2401 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 2461 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc 2521 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 2581 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc 2641 tccctttggg ccgcctcccc gcctggaaac gggggaggct aactgaaaca cggaaggaga 2701 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt 2761 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc 2821 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc 2881 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata 2941 gcagatctgc gcagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta 3001 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 3061 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 3121 gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc 3181 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 3241 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 3301 acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc 3361 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg 3421 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 3481 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 3541 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 3601 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 3661 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag 3721 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg 3781 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg 3841 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa 3901 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg 3961 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt 4021 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa 4081 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc 4141 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg 4201 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg 4261 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg 4321 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg 4381 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact 4441 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg 4501 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc 4561 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct 4621 ggggttcgcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc 4681 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg 4741 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca 4801 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 4861 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata 4921 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat 4981 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 5041 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 5101 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 5161 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 5221 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 5281 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 5341 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 5401 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 5461 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 5521 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg 5581 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 5641 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 5701 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 5761 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct 5821 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 5881 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 5941 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 6001 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 6061 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 6121 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 6181 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 6241 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 6301 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 6361 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 6421 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 6481 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 6541 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 6601 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 6661 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 6721 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 6781 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 6841 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 6901 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 6961 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 7021 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 7081 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc 7141 tatggtcgac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc 7201 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc 7261 aaggcttgac cgacaattgc atgaagaatc tgcttagg //