LOCUS V025774 6571 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V025774 VERSION V025774 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 744..770 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 774..800 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 804..830 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 840..881 /codon_start=1 /product="epitope tag from simian virus 5" /transl_table=1 /translation="GKPIPNPLLGLDST" /label="V5 tag" CDS 888..1244 /label="SNRPD1(NM_006938)" /note="SNRPD1(NM_006938)" /gene="SNRPD1" misc_feature 1347..1935 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 2001..2049 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 2251..2679 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2693..3022 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3089..3883 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4059..4180 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4229..4245) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4253..4269 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4277..4307) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4322..4343 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4631..5219) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5390..6250) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6251..6355) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg 661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg 721 actctagagc tagcgccacc atgtacccat acgacgtacc tgattatgca acttatcctt 781 atgatgtacc tgattatgct agctatcctt atgatgtccc agactacgca ggcggaggtg 841 gtaagcctat ccctaaccct ctcctcggtc tcgattctac aggatccatg aagctcgtga 901 gatttttgat gaaattgagt catgaaactg taaccattga attgaagaac ggaacacagg 961 tccatggaac aatcacaggt gtggatgtca gcatgaatac acatcttaaa gctgtgaaaa 1021 tgaccctgaa gaacagagaa cctgtacagc tggaaacgct gagtattcga ggaaataaca 1081 ttcggtattt tattctacca gacagtttac ctctggatac actacttgtg gatgttgaac 1141 ctaaggtgaa atctaagaaa agggaagctg ttgcaggaag aggcagagga agaggaagag 1201 gaagaggacg tggccgtggc agaggaagag ggggtcctag gcgataataa ttctgcagat 1261 atccagcaca gtggcggccg ctcgagtcta gagggcccct taagggttcg atccctaccg 1321 gttagtaatg agtttgatat ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat 1381 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 1441 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 1501 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 1561 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 1621 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 1681 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 1741 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 1801 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 1861 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 1921 gggccgcctc cccgcctgga aacgggggag gctaactgaa acacggaagg agacaatacc 1981 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt 2041 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga 2101 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt 2161 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcagatc 2221 tgcgcagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg 2281 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 2341 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 2401 atcggggcat ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 2461 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 2521 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 2581 accctatctc ggtctattct tttgatttat aagggatttt ggggatttcg gcctattggt 2641 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca 2701 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 2761 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 2821 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 2881 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 2941 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt 3001 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga 3061 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc 3121 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg 3181 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac 3241 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc 3301 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg 3361 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga 3421 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg 3481 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg 3541 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt 3601 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc 3661 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg 3721 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga 3781 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc 3841 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc 3901 gcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg 3961 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc 4021 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata 4081 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 4141 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga 4201 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 4261 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 4321 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 4381 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 4441 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 4501 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 4561 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 4621 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 4681 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 4741 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 4801 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg 4861 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 4921 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 4981 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 5041 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 5101 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 5161 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 5221 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 5281 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 5341 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 5401 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 5461 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 5521 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 5581 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 5641 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 5701 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 5761 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 5821 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 5881 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 5941 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 6001 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 6061 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 6121 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 6181 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 6241 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 6301 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 6361 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtc 6421 gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg 6481 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt 6541 gaccgacaat tgcatgaaga atctgcttag g //