LOCUS V031101 5787 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V031101 VERSION V031101 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..1934 /label="Or4c3d(NM_020515)" /note="Or4c3d(NM_020515)" /gene="Or4c3d" CDS 1953..1982 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 2001..2024 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(2044..2060) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 2071..2693 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(2842..3430) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 3759..3806 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(4038..4832) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(4867..5224) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(5226..5330) /gene="bla" /label="AmpR promoter" rep_origin 5357..5785 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat ggatagtcca cgcaatgtca cagaattttt catgctgggt ctctcccaga 1081 acccccaggt gcagaggatg ttgtttgggc tttttctgct ggtcttcctg gtctctgttg 1141 ggggcaacat gctcatcatt atcacgatta ccttcagccc caccctaggt tctcctatgt 1201 actttttcct ctcatatctt tcctttattg atacttgcta ttcctcttgt atgacaccca 1261 aactcatcgc tgactccctg catgagggaa gagccatctc ttttgagggc tgcttggccc 1321 agttctttgt ggctcattta ttgggaggaa ctgagatcat tctgctcaca gtgatggcct 1381 atgaccgcta tgtggccatc tgcaagcccc tacactatac caccaccatg accaggcatg 1441 tctgtattgt gctggtggca gtggcttggc tgggtggcat cctgcattca acagctcagc 1501 tctttctggt ccttcagttg cccttctgtg gtcccaatgt gataaaccac tttgtgtgtg 1561 acttgtatcc tttactggag ctggcctgca ctgacacata tgtcattggc ctgctggtgg 1621 tggccaacag tggtgtgatt tgcctgttga acttcctcat gctggctgcc tcgtacattg 1681 tcatcctgcg cactttgagg tcccacagtg cagagggaag acggaaagct ctgtctacct 1741 gtggagccca cttcactgtt gttgctttgt tctttgtgcc ctgtatattt atttacatga 1801 gaccatcatc tactctgtcc atagacaaaa tagtagctgt gttttattgt attttgacac 1861 ccatgttcaa ccccctgatt tatactctga gaaatgcaga ggtgaaaaat gccatgaaga 1921 atctctggag aaaaacgcgt acgcggccgc tcgagcagaa actcatctca gaagaggatc 1981 tggcagcaaa tgatatcctg gattacaagg atgacgacga taaggtttaa acggccggcc 2041 gcggtcatag ctgtttcctg aacagatccc gggtggcatc cctgtgaccc ctccccagtg 2101 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta 2161 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg 2221 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa 2281 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa 2341 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc 2401 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc 2461 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg 2521 tgaaccactg ctcccttccc tgtccttctg attttaaaat aactatacca gcaggaggac 2581 gtccagacac agcataggct acctggccat gcccaaccgg tgggacattt gagttgcttg 2641 cttggcactg tcctctcatg cgttgggtcc actcagtaga tgcctgttga attgggtacg 2701 cggccagcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 2761 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 2821 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 2881 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 2941 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 3001 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 3061 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 3121 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 3181 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 3241 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 3301 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 3361 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 3421 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 3481 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 3541 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaacctg aggctatggc 3601 agggcctgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttggggggtg 3661 gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg gtggggtatc 3721 gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc caacaccgtg 3781 cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt 3841 ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac tccagcatga gatccccgcg 3901 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa 3961 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc 4021 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc 4081 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc 4141 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcgatccgc cacacccagc 4201 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag 4261 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg 4321 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga 4381 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg 4441 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc 4501 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc 4561 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg 4621 gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg 4681 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag 4741 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga 4801 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga 4861 tcgatctttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc 4921 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg 4981 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat 5041 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga 5101 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg 5161 ggagcctggg gactttccac accctaactg acacacattc cacagctggt tctttccgcc 5221 tcaggactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 5281 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 5341 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 5401 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 5461 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 5521 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 5581 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 5641 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 5701 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 5761 atttaacaaa aatttaacgc gaatttt //