LOCUS       V031286                 6162 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031286
VERSION     V031286
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2309
                     /label="Ngfr(NM_033217)"
                     /note="Ngfr(NM_033217)"
                     /gene="Ngfr"
     CDS             2328..2357
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2376..2399
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2419..2435)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2446..3068
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3217..3805)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4134..4181
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4413..5207)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5242..5599)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5601..5705)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5732..6160
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gaggagggca ggtgctgcct gcagcgccat ggaccggctg cgcctgctgc
     1081 tgctgctgct gctgcttcta ggggtgtcct ttggaggtgc caaggagaca tgttccacag
     1141 gcatgtacac ccacagtgga gagtgctgca aagcctgcaa cctgggcgaa ggtgtggccc
     1201 agccttgcgg agccaaccag accgtgtgtg aaccctgcct ggacagtgtt acgttctctg
     1261 acgtggtgag cgccaccgag ccgtgcaagc cgtgcaccga gtgcctgggc ctgcagagta
     1321 tgtccgctcc ctgtgtggag gcagacgatg ccgtgtgccg atgctcctat ggctactacc
     1381 aggacgagga gactggccgc tgcgaggctt gcagcgtgtg cggggtgggc tcaggactcg
     1441 tgttctcctg ccaggacaaa cagaacacag tgtgtgaaga gtgcccagag ggcacatact
     1501 cagatgaagc caaccacgtg gacccgtgcc taccctgcac ggtgtgcgag gacactgagc
     1561 gccagttacg cgagtgcacg ccctgggctg acgccgaatg cgaggagatc cctggccgat
     1621 ggatcacaag gtctacgccc ccggagggct ctgacgtcac aacacccagc acccaggagc
     1681 cggaggcacc tccagagcga gacctcatag ccagcacagt ggccgatacg gtgaccactg
     1741 tgatgggcag ctcccagcct gtagtgaccc gaggcaccgc tgacaacctc attcctgtct
     1801 attgctccat cttggctgct gtggttgtgg gccttgtggc ctatattgct ttcaagagat
     1861 ggaacagctg caagcaaaat aaacaaggag ccaacagccg gccggtgaac cagacacccc
     1921 caccagaggg agagaaactg cacagcgaca gcggcatctc tgtggacagc cagagcctgc
     1981 acgaccagca gacccacaca cagactgcct caggccaagc cctcaagggt gatggcaacc
     2041 tctacagtag cctgcccctg accaagcgtg aggaggtcga gaagctgctc aatggtgaca
     2101 cctggcgaca tctggcaggc gagctgggct accagccgga gcatatagac tcctttaccc
     2161 acgaggcctg cccagtccga gccctgctgg ccagctgggg tgcccaggac agcgcgacgc
     2221 tcgatgccct tttagccgcc ctgcgacgca tccagagagc tgacattgtg gagagcctgt
     2281 gcagcgagtc cactgccacg tcccctgtga cgcgtacgcg gccgctcgag cagaaactca
     2341 tctcagaaga ggatctggca gcaaatgata tcctggatta caaggatgac gacgataagg
     2401 tttaaacggc cggccgcggt catagctgtt tcctgaacag atcccgggtg gcatccctgt
     2461 gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt
     2521 gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta taatattatg
     2581 gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc
     2641 ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc
     2701 gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca
     2761 tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg
     2821 gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg
     2881 ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt aaaataacta
     2941 taccagcagg aggacgtcca gacacagcat aggctacctg gccatgccca accggtggga
     3001 catttgagtt gcttgcttgg cactgtcctc tcatgcgttg ggtccactca gtagatgcct
     3061 gttgaattgg gtacgcggcc agcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3121 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3181 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3241 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3301 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3361 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3421 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3481 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3541 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3601 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     3661 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3721 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3781 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3841 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3901 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3961 acctgaggct atggcagggc ctgccgcccc gacgttggct gcgagccctg ggccttcacc
     4021 cgaacttggg gggtggggtg gggaaaagga agaaacgcgg gcgtattggc cccaatgggg
     4081 tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa
     4141 cgacccaaca ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc gggttccttc
     4201 cggtattgtc tccttccgtg tttcagttag cctcccccta gggtgggcga agaactccag
     4261 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc
     4321 caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc
     4381 ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag
     4441 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
     4501 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcga
     4561 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
     4621 atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgctc
     4681 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
     4741 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
     4801 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
     4861 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
     4921 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa
     4981 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg
     5041 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
     5101 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
     5161 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct
     5221 catcctgtct cttgatcgat ctttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta
     5281 cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag
     5341 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
     5401 taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     5461 tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat
     5521 acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca cattccacag
     5581 ctggttcttt ccgcctcagg actcttcctt tttcaatatt attgaagcat ttatcagggt
     5641 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     5701 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     5761 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5821 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5881 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5941 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6001 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6061 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     6121 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt tt
//
