LOCUS       V039316                12003 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039316
VERSION     V039316
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..6683
                     /label="Myh10(NM_175260)"
                     /note="Myh10(NM_175260)"
                     /gene="Myh10"
     CDS             6690..6755
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    6779..7367
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    7433..7481
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7683..8111
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        8125..8454
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8521..9315
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    9491..9612
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(9661..9677)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9685..9701
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9709..9739)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9754..9775
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10063..10651)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10822..11682)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11683..11787)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatggc ccagagaact ggactggagg
      781 atcccgagag gtatctcttt gtggacaggg ctgtcatcta caaccctgcc actcaagctg
      841 actggacagc taaaaagctg gtgtggattc catcggaacg ccatggtttt gaggcagcta
      901 gtattaaaga agagcggggc gatgaggtta tggtggagct ggcagagaat gggaagaaag
      961 caatggtcaa caaagatgac attcagaaga tgaacccacc aaagttctcc aaggtggagg
     1021 atatggcaga gctgacatgc ttgaacgaag cctctgtctt acataatttg aaggaccgct
     1081 actattcagg acttatctat acttactctg gactcttctg tgtggtgata aatccttaca
     1141 agaaccttcc aatttactct gagaatatta ttgaaatgta tagagggaag aaacgccatg
     1201 agatgccacc acacatctac gccatatcag agtctgctta cagatgcatg cttcaagatc
     1261 gtgaggacca gtcaattcta tgcacgggtg aatcgggtgc cgggaagaca gaaaatacca
     1321 agaaagtcat tcagtacctt gcccacgttg cttcttctca caaaggaaga aaggaccata
     1381 atattcctgg ggaacttgaa cggcagcttt tacaagcaaa tccaattctg gaatcctttg
     1441 gaaatgcgaa gactgtgaaa aatgataact catctcgctt tggcaagttt atccggatca
     1501 actttgatgt aactggctat attgttgggg ccaacattga aacatacctt ctggaaaagt
     1561 ctcgtgctgt tcgtcaagct aaagatgagc gtacatttca tatcttttat cagttgctct
     1621 ctggagcagg ggaacacctg aaatccgact tactcctgga aggtttcaac aactacagat
     1681 tcctctccaa tggctatatt cctattcctg gacagcaaga caaggataac ttccaggaga
     1741 ccatggaagc catgcacatc atgggcttct ctcacgaaga gatcctctca atgcttaaag
     1801 tcgtatcttc agtgctgcag tttggaaaca tctctttcaa aaaggagaga aacactgacc
     1861 aagcctccat gccggagaac acagtcgcac agaagctctg ccacctgctc gggatgaatg
     1921 tgatggagtt cactcgggct atcctcacgc ccaggatcaa ggttggccgg gattacgtac
     1981 agaaagccca gaccaaagag caggctgatt ttgcagtgga agcattggca aaagctacct
     2041 atgagcggtt gtttcgctgg ctcgttcacc gcatcaataa agcgctggat aggaccaaac
     2101 gccagggagc ttccttcatt gggatcctgg atattgctgg ttttgaaatt tttgagctga
     2161 actccttcga gcagctgtgc atcaactaca ccaacgagaa gctgcagcag ctgttcaacc
     2221 acaccatgtt catcctggag caggaggagt accagcgaga gggcatcgag tggaacttta
     2281 tcgacttcgg cctggacctg cagccctgca tcgacctgat agagagacct gccaatcccc
     2341 ctggcgtgct ggccctcctg gatgaagaat gctggttccc caaagctaca gataaaacat
     2401 ttgttgaaaa gctggttcag gagcaaggtt cccactccaa gtttcagaag ccgcgccaac
     2461 tgaaagacaa agccgacttc tgcatcatcc actacgcggg gaaggtggac tataaggcag
     2521 atgagtggct gatgaagaac atggacccgc tgaatgacaa cgtggccacc ctcctgcacc
     2581 agtcctcgga cagatttgtg gctgagcttt ggaaggacgt ggaccgaatt gtaggtctgg
     2641 atcaagtcac tgggatgact gagaccgcgt ttggctctgc atacaaaacc aagaagggca
     2701 tgttccgaac cgtcgggcag ctctacaagg agtctctcac caagctgatg gcaactctcc
     2761 gcaacaccaa ccccaacttc gtccgctgca tcattccaaa tcacgagaag cgggctggga
     2821 aactggaccc gcacctcgtg ctcgatcagc ttcgctgtaa cggcgtcctg gaagggatcc
     2881 ggatctgtcg ccaggggttc cccaaccgga tagttttcca ggaattcaga cagagatatg
     2941 agatcctaac tcccaatgct attcctaaag gcttcatgga tggcaaacag gcgtgtgagc
     3001 gaatgatccg agctttagaa ctggacccaa acctgtatag aattggacag agcaagatat
     3061 ttttccgagc tggagttttg gcgcacttag aagaagaaag agatttaaaa atcactgata
     3121 tcatcatctt tttccaagct gtatgcagag gctacctcgc ccgaaaggcc tttgccaaga
     3181 aacagcaaca actaagtgcc ttaaaggtct tgcagcggaa ctgtgcggcg tacctgaagc
     3241 tgcgacactg gcagtggtgg cgtgtcttca cgaaggtgaa gcctctcctc caagtgaccc
     3301 gccaggagga agaactccag gcaaaagatg aggagctgct gaaggtgaaa gagaagcaga
     3361 caaaagtgga aggggagctt gaggagatgg agcggaagca ccagcagctg ctggaagaga
     3421 agaatatcct ggcagaacaa ctgcaagccg agaccgagct cttcgctgaa gcagaagaga
     3481 tgagagcaag gcttgctgcc aaaaagcagg aactggagga gattctccat gacctcgagt
     3541 ccagggtgga ggaggaggaa gagcggaacc agatcctaca gaatgagaag aagaagatgc
     3601 aggcgcacat tcaggaccta gaagaacaac tggatgagga ggagggggcc cggcaaaagc
     3661 tgcagctgga gaaggtgaca gcagaggcta aaatcaagaa gatggaagag gaggttctgc
     3721 ttctcgaaga ccagaattcc aaatttatca aagaaaagaa actcatggaa gaccgaattg
     3781 ctgagtgttc ctctcagctg gctgaagagg aagaaaaggc aaaaaacttg gccaaaatca
     3841 ggaataagca agaagtgatg atctcggact tagaagaacg cttgaagaag gaggagaaaa
     3901 ctcgacagga actggaaaag gccaaacgga agctggatgg ggaaacaacc gatctgcagg
     3961 accagatcgc tgagctgcag gcacaggtcg atgagctcaa agtccagttg accaagaagg
     4021 aggaggagct tcagggggcg ctggccagag gagatgatga gacactgcac aagaataatg
     4081 cacttaaagt tgcacgggag ctgcaggccc aaatcgcaga gctccaggaa gactttgagt
     4141 ctgaaaaggc ttcaaggaac aaggctgaga aacaaaaacg ggacttgagt gaggagctgg
     4201 aagctctgaa gacagagctg gaggacaccc tagacaccac agcagctcag caggaactcc
     4261 gcacaaaacg tgagcaggaa gtggcagagc tgaagaaggc tcttgaggat gaaactaaga
     4321 accacgaagc tcagatccag gacatgagac agaggcatgc cacagcgctg gaggagcttt
     4381 ccgagcagct ggagcaagcg aaaaggttca aagccaacct ggagaagaac aaacagggcc
     4441 tggagacaga caacaaggag ctggcgtgtg aggtgaaggt gctgcagcag gtgaaggcgg
     4501 agtcagagca caagaggaag aagctggatg cccaggtcca ggagctccat gccaaggtgt
     4561 cagagggtga caggctcagg gtagagctgg ccgagaaagc aaacaagcta cagaatgagc
     4621 tggataatgt gtcaaccctg ctggaagaag ctgagaagaa aggtattaag tttgcgaagg
     4681 atgcagctgg tctcgagtct caactacagg acacacagga gctccttcag gaagagacac
     4741 ggcagaaact gaacctgagc agtcggatcc ggcagctgga ggaggagaag aacagccttc
     4801 aggagcagca ggaggaggag gaggaggcca ggaagaacct ggagaagcag gtgttggctc
     4861 tgcagtccca gctggctgac accaagaaga aagtggacga tgacctgggg acaatcgaga
     4921 gtttggagga agccaaaaag aaactgctca aggatgtgga ggcgctgagc cagcggctgg
     4981 aggagaaggt cctggcgtat gacaagctgg agaagaccaa gaaccggctg caacaagaac
     5041 tggatgacct gacggtggac ctggaccacc agcgccagat cgtctccaac ttggagaaga
     5101 aacagaagaa gttcgaccag ctgttggcag aagaaaaggg catctctgct cgctatgcag
     5161 aagagcggga ccgggctgaa gctgaggcca gagagaaaga aaccaaagcg ctctccctgg
     5221 cgcgggccct tgaggaggcc ttggaggcga aggaggaatt cgagaggcag aacaagcagc
     5281 ttcgagcaga catggaagac ctgatgagct ctaaagacga tgtggggaag aacgtccacg
     5341 agcttgagaa atccaagcga gccttggagc agcaggtgga ggagatgcgg acccagctgg
     5401 aggagctgga ggacgagctg caggccactg aggatgccaa gctccgcctg gaagtcaaca
     5461 tgcaggccat gaaggcccag tttgagaggg acctgcaaac ccgagatgag cagaatgaag
     5521 aaaagaagcg gctgctgctt aagcaggtgc gggagctcga ggcagagctg gaggatgagc
     5581 ggaaacagcg ggcactggct gtggcgtcaa agaagaagat ggagatagac ctgaaggacc
     5641 tggaggctca gatcgaggct gcgaacaaag cccgggatga agtgatcaag cagcttcgca
     5701 aacttcaggc acagatgaag gattaccagc gtgaactaga agaggctcga gcatctagag
     5761 atgagatttt tgctcaatcc aaagaaagtg aaaagaaact gaagagtcta gaagcagaaa
     5821 ttcttcagct gcaagaggag ctggcctcat ccgagcgagc ccgccgacac gcagagcagg
     5881 agcgagacga gctggctgat gagatcgcca acagcgcctc tggaaagtct gcgctgttgg
     5941 atgagaagcg gcgcctggaa gcgcggatcg cacagctgga agaggagctg gaggaggagc
     6001 agagcaacat ggagctgctc aatgaccgct tccgcaagac cacgctgcag gtggacacac
     6061 tgaacacaga gctggcagca gagcgcagcg ctgcccagaa gagtgacaat gcccgccagc
     6121 agctggagcg acaaaacaag gagctgaagg ccaagctgca ggagctggag ggggcagtca
     6181 agtccaagtt caaggctacc atctcagccc tggaagccaa gattgggcag ctggaggagc
     6241 agcttgagca ggaagccaag gagcgagcag ctgccaacaa actagtccgt cgaacagaga
     6301 agaaactgaa agaaatcttc atgcaggttg aagacgagcg tcggcatgcg gatcagtata
     6361 aggagcagat ggagaaggct aatgccagga tgaagcagct taaacgacag ttggaagagg
     6421 ctgaggaaga ggccacacgt gccaacgcat ctcggcgtaa actccaaagg gagctggacg
     6481 acgccactga ggccaatgaa ggcctgagcc gcgaggtcag cactctcaag aaccggctca
     6541 ggcggggcgg tccaatcagc ttttcttcaa gccgatctgg ccggcgccag ctgcacattg
     6601 agggggcatc gctagagctg tcagatgacg acacagaaag taagaccagt gatgtcaatg
     6661 acacacagcc accccaatca gaaggatccg actacaaaga ccatgacggt gattataaag
     6721 atcatgacat cgactacaag gatgacgatg acaagtagtg agggcccgat atctcgacaa
     6781 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6841 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6901 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6961 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     7021 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     7081 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     7141 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     7201 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     7261 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     7321 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaaacggggg
     7381 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     7441 aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
     7501 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
     7561 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
     7621 cgtcggggcg gcaggccctg ccatagcaga tctgcgcagc tggggctcta gggggtatcc
     7681 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     7741 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     7801 cacgttcgcc ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt
     7861 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     7921 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     7981 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     8041 ataagggatt ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     8101 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     8161 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     8221 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     8281 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     8341 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     8401 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     8461 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
     8521 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     8581 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     8641 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     8701 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     8761 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     8821 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     8881 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     8941 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     9001 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     9061 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     9121 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     9181 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     9241 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     9301 gacgagttct tctgagcggg actctggggt tcgcgaaatg accgaccaag cgacgcccaa
     9361 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     9421 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     9481 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     9541 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     9601 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     9661 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     9721 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     9781 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     9841 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9901 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9961 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    10021 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    10081 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    10141 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    10201 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
    10261 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    10321 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    10381 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    10441 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10501 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10561 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10621 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    10681 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    10741 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    10801 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    10861 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10921 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10981 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    11041 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    11101 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    11161 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    11221 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    11281 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    11341 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    11401 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    11461 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    11521 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    11581 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    11641 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    11701 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    11761 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
    11821 cggatcggga gatctcccga tcccctatgg tcgactctca gtacaatctg ctctgatgcc
    11881 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
    11941 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
    12001 agg
//
