LOCUS V031083 5721 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V031083 VERSION V031083 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..1868 /label="Klra7(NM_001110323)" /note="Klra7(NM_001110323)" /gene="Klra7" CDS 1887..1916 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 1935..1958 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(1978..1994) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 2005..2627 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(2776..3364) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 3693..3740 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(3972..4766) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(4801..5158) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(5160..5264) /gene="bla" /label="AmpR promoter" rep_origin 5291..5719 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat gagtgagcag gaggtcactt actcaactgt gagatttcat gagtcttcaa 1081 ggttgcagaa actagtgagg actgaggagc ctcaaaggcc cagagaagct tgctacagag 1141 agtattcagt cccctggaag ctcattgtga tagcttgtgg aatcctctgt ttccttctcc 1201 tggtaactgt tgcattgttg gcaataacga tttttcagca tagtcaacaa aaacatgaac 1261 tacaggaaac tctaaattgc cacgataact gcagccccac gcaaagtgac gtcaacttga 1321 aggatgaact gctgagaaat aagtctatag agtgtaggcc aggcaatgat cttctggaat 1381 ccctcagcag ggatcagaac agatggtaca gtgaaaccaa gactttttca gattcctcac 1441 agcacacagg tgttcacaaa agacccatct ccaaggcaga ggggaagggt agaggttttg 1501 aaaaatattg gttctgttat ggtataaaat gttattattt caacatggac agaaaaacgt 1561 ggagtggatg taaacagacc tgccagattt ccagcttatc ccttctgaag atagacaatg 1621 aggatgaact gaagttcctt cagaacctgg ctccttcaga catttcctgg attggattgt 1681 catatgacaa taagaaaaaa gattgggtat ggattgacaa tggcccatct aaacttgcct 1741 tgaacacaac gaaatataat ataagagatg gattatgtat gtcgttatct aaaacaagac 1801 tagacaatgg ggactgtgat aaatcataca tctgtatttg tggtaagaga ctggataaat 1861 ttcctcatac gcgtacgcgg ccgctcgagc agaaactcat ctcagaagag gatctggcag 1921 caaatgatat cctggattac aaggatgacg acgataaggt ttaaacggcc ggccgcggtc 1981 atagctgttt cctgaacaga tcccgggtgg catccctgtg acccctcccc agtgcctctc 2041 ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc 2101 atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg 2161 agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc 2221 tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt 2281 ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa 2341 tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc 2401 taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc 2461 actgctccct tccctgtcct tctgatttta aaataactat accagcagga ggacgtccag 2521 acacagcata ggctacctgg ccatgcccaa ccggtgggac atttgagttg cttgcttggc 2581 actgtcctct catgcgttgg gtccactcag tagatgcctg ttgaattggg tacgcggcca 2641 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 2701 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 2761 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 2821 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 2881 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 2941 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 3001 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 3061 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 3121 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 3181 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 3241 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 3301 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 3361 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 3421 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 3481 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa cctgaggcta tggcagggcc 3541 tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg 3601 ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga 3661 gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt 3721 attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt 3781 ttcagttagc ctccccctag ggtgggcgaa gaactccagc atgagatccc cgcgctggag 3841 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc 3901 ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc 3961 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg 4021 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca 4081 gcaatatcac gggtagccaa cgctatgtcc tgatagcgat ccgccacacc cagccggcca 4141 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg 4201 ccatgggtca cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt 4261 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct 4321 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta 4381 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca 4441 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc 4501 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc 4561 cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg caccggacag gtcggtcttg 4621 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg 4681 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct 4741 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcgatc 4801 tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc 4861 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg 4921 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc 4981 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc 5041 acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc 5101 tggggacttt ccacacccta actgacacac attccacagc tggttctttc cgcctcagga 5161 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 5221 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 5281 gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 5341 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 5401 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 5461 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 5521 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 5581 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 5641 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 5701 caaaaattta acgcgaattt t //