LOCUS       V031270                 7014 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031270
VERSION     V031270
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..3161
                     /label="Irak1(NM_001177975)"
                     /note="Irak1(NM_001177975)"
                     /gene="Irak1"
     CDS             3180..3209
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3228..3251
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3271..3287)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3298..3920
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4069..4657)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4986..5033
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(5265..6059)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(6094..6451)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6453..6557)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6584..7012
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggccgggggg ccgggccccg gggagcctgt ggttccaggc gcccagcact
     1081 tcttgtacga ggtgccaccc tgggttatgt gccgcttcta caaagtgatg gacgccctgg
     1141 agcccgccga ctggtgccag ttcgcggcct tgatcgtgcg cgaccagaca gagctgcggc
     1201 tgtgcgagcg ctccgagcag cgcacagcca gtgttctgtg gccctggatc aaccgcaacg
     1261 cgcgcgtagc tgacctcgtt cacatcctca cgcacctgca gctgctgcgt gcgagggaca
     1321 tcatcacagc ctggcaccct cctgcccccg ttgtgccccc aagcaccgct gccccaaggc
     1381 ccagcagcat ctctgcaggc tctgaggccg gggactggag cccccggaaa ttgcagtcct
     1441 ctgcctccac cttcctctcc ccagcttttc caggctccca gacccattct gagtcagagc
     1501 tcctccaggt tccactccct gtttccctcg ggccaccact accatcttca gccccttcct
     1561 ccaccaagca gtcaagccca gagagcccag tgtctggcct ccaaagagcc catccctccc
     1621 cgttttgctg gcccttctgt gagatttccc aaggcacctg caacttctct gaagaactca
     1681 ggattggaga gggtggtttt ggatgtgtgt accgagcagt catgagaaat actacatatg
     1741 ctgtgaagag actgaaggag gaagctgacc tagagtggac tatggtgaaa cagagcttct
     1801 taacagaggt ggaacagcta tcaaggtttc gtcacccaaa tatcgtagac tttgctggct
     1861 actgtgcaga gagtggctta tactgccttg tttatggctt cttgcccaat ggctccttgg
     1921 aggatcagct ccaccttcag acccaggcct gctccccact ttcctggcct caacgactgg
     1981 acattcttct gggcacagcc cgggctattc agtttttaca tcaggatagc cccagcctta
     2041 tccatggaga catcaagagt tctaacgtgc ttctggatga gagactgatg cccaagctgg
     2101 gagactttgg cctggctcgt ttcagccgct ttgcgggggc caaagcaagc cagagcagta
     2161 ctgtggcccg gacttccaca gttcgaggta ccctcgccta cttgcctgag gagtacatca
     2221 agacaggccg actggctgtg gacaccgata ccttcagctt tggggtggta atactggaga
     2281 cccttgctgg tcagagggct gtgaggacac aaggtgcaaa gaccaagtat ttgaaagacc
     2341 tgattgaaga tgaggctgaa gaggctggag tgaccctgaa aagcacccag cctactctgt
     2401 gggtgggtgt agccacggat gcttgggctg ctccaattgc tgcccagatc tataagaagc
     2461 acctggactc cagacctggg ccctgcccac cccagttggg cctggccctg gctcaactag
     2521 cttgctgctg catgcaccgt cgggccaaga agaggccccc catgacccag gtatacaaga
     2581 gactagaagg gcttcaggca gggcctccct gggagctaga ggttgccggc catggctccc
     2641 cttccccaca ggagaactcc tacatgtcta ccactggcag tgcccagagt ggggatgaac
     2701 catggcagcc tctagtagtg accacaagag ccccagccca ggctgcccag caactccaga
     2761 gaagtcccaa ccagccagtg gaaagtgatg agagtgttcc cggcctctct gctaccctgc
     2821 attcctggca cttgactcca ggttcccacc caagcccagc gtccttcaga gaggctagct
     2881 gtacccaggg aggcactacc agagaatcaa gtgtgaggag tagcccaggc ttccagccta
     2941 caaccatgga aggctcaccc acgggcagct catccctgct gtcatcagaa ccaccacaga
     3001 tcatcatcaa cccagcccga cagaagatgg tacaaaagct ggctctttat gaagaagggg
     3061 tcttggatag cctgcaactg ctgtcatcag gctttttccc aggcttggat ttagaacctg
     3121 aaaagagcca gggacctgaa gaaagtgatg aattccagag cacgcgtacg cggccgctcg
     3181 agcagaaact catctcagaa gaggatctgg cagcaaatga tatcctggat tacaaggatg
     3241 acgacgataa ggtttaaacg gccggccgcg gtcatagctg tttcctgaac agatcccggg
     3301 tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg
     3361 cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc
     3421 tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc
     3481 tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc
     3541 actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg
     3601 ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt
     3661 ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg
     3721 cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt
     3781 ttaaaataac tataccagca ggaggacgtc cagacacagc ataggctacc tggccatgcc
     3841 caaccggtgg gacatttgag ttgcttgctt ggcactgtcc tctcatgcgt tgggtccact
     3901 cagtagatgc ctgttgaatt gggtacgcgg ccagcggcga gcggtatcag ctcactcaaa
     3961 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     4021 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4081 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4141 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4201 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4261 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4321 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4381 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4441 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4501 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     4561 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     4621 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     4681 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     4741 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     4801 tatatatgag taacctgagg ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc
     4861 tgggccttca cccgaacttg gggggtgggg tggggaaaag gaagaaacgc gggcgtattg
     4921 gccccaatgg ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg
     4981 tttatgaaca aacgacccaa caccgtgcgt tttattctgt ctttttattg ccgtcatagc
     5041 gcgggttcct tccggtattg tctccttccg tgtttcagtt agcctccccc tagggtgggc
     5101 gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac
     5161 gattccgaag cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag
     5221 gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca
     5281 agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg
     5341 aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg
     5401 tcctgatagc gatccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca
     5461 ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg
     5521 tcgggcatgc tcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct
     5581 tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg
     5641 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc
     5701 attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc
     5761 tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc
     5821 acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc
     5881 agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct
     5941 gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg
     6001 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg
     6061 cgaaacgatc ctcatcctgt ctcttgatcg atctttgcaa aagcctaggc ctccaaaaaa
     6121 gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat
     6181 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
     6241 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
     6301 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
     6361 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
     6421 cacattccac agctggttct ttccgcctca ggactcttcc tttttcaata ttattgaagc
     6481 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     6541 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc
     6601 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     6661 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     6721 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     6781 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     6841 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     6901 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     6961 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa tttt
//
