LOCUS V029943 8401 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V029943 VERSION V029943 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..3060 /label="IFI16(NM_001364867)" /note="IFI16(NM_001364867)" /gene="IFI16" CDS 3067..3093 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3097..3123 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3127..3153 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" misc_feature 3177..3765 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 3831..3879 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 4081..4509 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4523..4852 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4919..5713 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5889..6010 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(6059..6075) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6083..6099 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6107..6137) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6152..6173 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6461..7049) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7220..8080) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8081..8185) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggg aaaaaaatac 721 aagaacattg ttctactaaa aggattagag gtcatcaatg attatcattt tagaatggtt 781 aagtccttac tgagcaacga tttaaaactt aatttaaaaa tgagagaaga gtatgacaaa 841 attcagattg ctgacttgat ggaagaaaag ttccgaggtg atgctggttt gggcaaacta 901 ataaaaattt tcgaagatat accaacgctt gaagacctgg ctgaaactct taaaaaagaa 961 aagttaaaag taaaaggacc agccctatca agaaagagga agaaggaagt ggatgctact 1021 tcacctgcac cctccacaag cagcactgtc aaaactgaag gagcagaggc aactcctgga 1081 gctcagaaaa gaaaaaaatc aaccaaagaa aaggctggac ccaaagggag taaggtgtcc 1141 gaggaacaga ctcagcctcc ctctcctgca ggagccggca tgtccacagc catgggccgt 1201 tccccatctc ccaagacctc attgtcagct ccacccaaca gttcttcaac tgagaacccg 1261 aaaacagtgg ccaaatgtca ggtaactccc agaagaaatg ttctccaaaa acgcccagtg 1321 atagtgaagg tactgagtac aacaaagcca tttgaatatg agaccccaga aatggagaaa 1381 aaaataatgt ttcatgctac agtggctaca cagacacagt tcttccatgt gaaggtttta 1441 aacaccagct tgaaggagaa attcaatgga aagaaaatca tcatcatatc agattatttg 1501 gaatatgata gtctcctaga ggtcaatgaa gaatctactg tatctgaagc tggtcctaac 1561 caaacgtttg aggttccaaa taaaatcatc aacagagcaa aggaaactct gaagattgat 1621 attcttcaca aacaagcttc aggaaatatt gtatatgggg tatttatgct acataagaaa 1681 acagtaaatc agaagaccac aatctacgaa attcaggatg atagaggaaa aatggatgta 1741 gtggggacag gacaatgtca caatatcccc tgtgaagaag gagataagct ccaacttttc 1801 tgctttcgac ttagaaaaaa gaaccagatg tcaaaactga tttcagaaat gcatagtttt 1861 atccagataa agaaaaaaac aaacccgaga aacaatgacc ccaagagcat gaagctaccc 1921 caggaacagc gtcagcttcc atatccttca gaggccagca caaccttccc tgagagccat 1981 cttcggactc ctcagatgcc accaacaact ccatccagca gtttcttcac caagaaaagt 2041 gaagacacaa tctccaaaat gaatgacttc atgaggatgc agatactgaa ggaagggagt 2101 cattttccag gaccgttcat gaccagcata ggcccagctg agagccatcc ccacactcct 2161 cagatgcctc catcaacacc aagcagcagt ttcttaacca cgaaaagtga agacacaatc 2221 tccaaaatga atgacttcat gaggatgcag atactgaagg aagggagtca ttttccagga 2281 ccgttcatga ccagcatagg cccagctgag agccatcccc acactcctca gatgcctcca 2341 tcaacaccaa gcagcagttt cttaaccacg ttgaaaccaa gactgaagac tgaacctgaa 2401 gaagtttcca tagaagacag tgcccagagt gacctcaaag aagtgatggt gctgaacgca 2461 acagaatcat ttgtatatga gcccaaagag cagaagaaaa tgtttcatgc cacagtggca 2521 actgagaatg aagtcttccg agtgaaggtt tttaatattg acctaaagga gaagttcacc 2581 ccaaagaaga tcattgccat agcaaattat gtttgccgca atgggttcct ggaggtatat 2641 cctttcacac ttgtggctga tgtgaatgct gaccgaaaca tggagatccc aaaaggattg 2701 attagaagtg ccagcgtaac tcctaaaatc aatcagcttt gctcacaaac taaaggaagt 2761 tttgtgaatg gggtgtttga ggtacataag aaaaatgtaa ggggtgaatt cacttattat 2821 gaaatacaag ataatacagg gaagatggaa gtggtggtgc atggacgact gaccacaatc 2881 aactgtgagg aaggagataa actgaaactc acctgctttg aattggcacc gaaaagtggg 2941 aataccgggg agttgagatc tgtaattcat agtcacatca aggtcatcaa gaccaggaaa 3001 aacaagaaag acatactcaa tcctgattca agtatggaaa cttcaccaga ctttttcttc 3061 ctcgagtacc catacgacgt acctgattat gcaacttatc cttatgatgt acctgattat 3121 gctagctatc cttatgatgt cccagactac gcatagtgag ggcccgatat ctcgacaatc 3181 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 3241 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 3301 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 3361 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 3421 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 3481 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 3541 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct 3601 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 3661 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 3721 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga aacgggggag 3781 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa 3841 aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc 3901 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt 3961 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg 4021 tcggggcggc aggccctgcc atagcagatc tgcgcagctg gggctctagg gggtatcccc 4081 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 4141 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 4201 cgttcgccgg ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta 4261 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 4321 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 4381 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 4441 aagggatttt ggggatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 4501 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 4561 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 4621 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 4681 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 4741 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga 4801 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 4861 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat 4921 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 4981 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 5041 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 5101 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 5161 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 5221 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 5281 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 5341 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 5401 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg 5461 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 5521 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 5581 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 5641 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 5701 cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg acgcccaacc 5761 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg 5821 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg 5881 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 5941 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 6001 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt 6061 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 6121 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 6181 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 6241 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 6301 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 6361 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 6421 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 6481 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 6541 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 6601 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct 6661 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 6721 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 6781 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 6841 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 6901 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 6961 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 7021 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 7081 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 7141 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 7201 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 7261 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 7321 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 7381 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 7441 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 7501 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 7561 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 7621 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 7681 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 7741 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 7801 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 7861 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 7921 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 7981 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 8041 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 8101 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 8161 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg 8221 gatcgggaga tctcccgatc ccctatggtc gactctcagt acaatctgct ctgatgccgc 8281 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag 8341 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag 8401 g //