LOCUS       V032098                14022 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032098
VERSION     V032098
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..8702
                     /label="HELZ2(NM_001037335)"
                     /note="HELZ2(NM_001037335)"
                     /gene="HELZ2"
     CDS             8709..8774
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    8798..9386
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    9452..9500
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      9702..10130
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        10144..10473
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             10540..11334
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    11510..11631
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(11680..11696)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11704..11720
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11728..11758)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11773..11794
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(12082..12670)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12841..13701)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13702..13806)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatggc tgtgtgggag gccgagcagc
      781 tgggtggcct ccagcggggg gacctgctca caccccctgc ccctgatggc gacgggcgca
      841 cggcccccct tggccagccc cctggggccc agctgtactg cccggcctgc ttggtcacct
      901 gccactctca ggaggccttc gagaaccact gcgcatcctc ggagcacgca cagatggtgg
      961 ccttcgacca ggccctgccc tgggagcacc gttccccacc cccgggactc tccaagttcg
     1021 agctctgccc aaagcctgac ctctgtgagt atggggacgc ctgcaccaag gcacactcag
     1081 cacaggagct gcaggagtgg gtccggcgca cgcaggctgt ggagctgcgg gggcaggcgg
     1141 cctggcagga cgggctggtg ccctaccagg agcggctgct ggccgagtac cagcgcagca
     1201 gcagtgaggt ccttgtgctg gcagagaccc ttgatggagt gcgtgtcacc tgcaaccagc
     1261 ccctgatgta ccaggcccag gagaggaaga cccagtacag ctggacgttt gccgtccact
     1321 ctgaggagcc cctgctacac gtggccctgc tgaagcagga gccaggagcc gacttctctc
     1381 tggtggctcc cggcctcccg ccaggccggc tctacgcacg gggtgagcgc ttccgtgtgc
     1441 ccagctccac tgccgacttc caggtgggag tgcgtgtgca ggctgcctcg ttcggcacct
     1501 ttgagcaatg ggtggtcttc gactttggcc gccggccggt gctgctacaa aagctggggc
     1561 tgcagctggg ccaggggcgt cgcccaggac cctgcaggaa tctggcgctc ggccaccctg
     1621 aggagatgga gcgctggcac actggcaacc gccacgtggt gcctggcgtg gagcggacgg
     1681 ccgagcagac ggccctgatg gccaagtaca agggccctgc cctggccctg gagttcaacc
     1741 gcagcagcgt ggcctcgggc cccatctcac caaccaacta tcggcagagg atgcaccagt
     1801 ttctctatga ggaggaggcg gctcagcagc agctggtggc caagctgacc ctgcggggcc
     1861 aggtgttcct gaagacggca ttgcagacgc cagcgctgaa catgctcttc gcgcctccgg
     1921 gagcactgta cgcagaggtc cccgtcccct cctccctgat gccagacaca gaccagggct
     1981 tcctgctggg ccgggcggtc agcacagccc tggtggcccc tgtacctgca cccgacaata
     2041 cggtgttcga ggtgcggctg gagaggcggg ccagctcaga gcaggcgctg tggctgctgc
     2101 ttccggcccg ctgctgcttg gccctggggc tgcagcctga ggcccgcctg gtcctggagg
     2161 tgcagttcca gattgacccg atgaccttcc gcctctggca ccaggcagtg gacacactgc
     2221 ctgaggagca gctggtggtg cccgacttgc ccacctgcgc cctgcccaga ccttggtctg
     2281 tcccaccctt gcggcgtggc aaccgcaagc aggagctggc cgtggcgctc atcgcgggct
     2341 ggggccctgg ggatgggagg cgtgtccccc cgctactcat ctatggcccc tttggcaccg
     2401 gcaagaccta cacgctggcc atggcctccc tggaggtcat ccggaggcct gaaaccaagg
     2461 tgctcatctg cacacacacc aacagtgccg ccgacatcta catccgggag tatttccaca
     2521 gccacgtcag cggcggccac cccgaggcca ctcctctccg tgtgatgtac acggaccggc
     2581 cgctgagcca gacggaccca gtcacgctgc agtactgttg cctgaccgac gaccgccagg
     2641 ctttccgccc gcccacacgg gcagagctgg cgcggcaccg cgtggtggtc accaccacct
     2701 cccaggcccg tgagctcagg gtgccggtcg gcttcttctc ccacattctc atcgatgagg
     2761 cggcccagat gctggagtgc gaggccctca ccccgctggc ctatgcctcg cacggcaccc
     2821 gcctcgtgct ggcgggcgac cacatgcagg tcacaccccg gctgttcagt gtggccaggg
     2881 cccgggcggc cgagcacacg ctgctgcacc gcctcttcct gtgctaccag caggagactc
     2941 acgaggtggc gcggcagagc cgcctggtct tccacgagaa ctaccgctgc acggacgcca
     3001 ttgtcagctt catctcgcgg cacttctacg tggccaaggg caaccccatc cacgccaggg
     3061 gcaaggttcc gccccacccc cggcactacc cgctcatgtt ctgccacgtg gcgggcagcc
     3121 cagaccggga catgtccatg gcgtcctggc tgaatctggc tgagattgcg caggtcgtcg
     3181 agaaggtgca ggaggcctac aacacctggc ccagctgctg gggcggccgc gagcagaggt
     3241 gcatctgtgt cgtttcccac ggtgcccagg tcagtgcact gaggcaggag ctgaggaggc
     3301 gggacctagg ccaggtgtct gtcggcagtt ttgagatcct gccagggcgg cagttccggg
     3361 tcgtggtgct cagcactgtg cacacctgcc agagcctgct cagccctggg gcactggccc
     3421 ctgagttctt caccgacgcc cgcgtgctca acaccgtcct gacccgcgcc cagtcccagc
     3481 tggtggtagt gggggacgcc gtggccctct gctccttcgg ggcctgcggc aagctctggg
     3541 agagcttcat ccgtgagtgc gtggagcggc acagtgtctg ccccgagggc ctgtccatgg
     3601 agcaggtcga gcagggtgtg gcgcagagac ggcgctggcc tccccgaggc acacaggctg
     3661 gggcagcggg gaactgggag gctgccccag agccagtagg ggacctggcc gaggagcagg
     3721 cggctgtggt gacggccatg gtgaaggcag agccgggaga tgaggctctg agcccagcat
     3781 cccgtgacat cacggcaacc acagcgcaga cggaggctgc ggcagcacca gcaggagacg
     3841 cagtgaagga agacgtggtg cccggggcct gtgcggcagg agcggctgct gcagcgggcg
     3901 tggagtccac ggaggctgag gatgcagagg ctgacttctg gccgtgggac ggggagctca
     3961 acgctgacga cgccatccta cgggagcttc tggacgagag ccagaaggtg atggtgaccg
     4021 tcggggagga cgggctgctg gacactgtcg ccaggcccga gtccctgcag caggcccggc
     4081 tgtacgagaa cctgcccccg gctgcgctac ggaagctgct gcacgcggag cccgagcggt
     4141 accgccactg ctctttcgtg ccagagacct tcgagcgggc gtcagccatc ccgctggacg
     4201 atgcctcctc gggccccatc caggtcaggg gccgcctgga ctgtgggatg gccttcgccg
     4261 gggatgaggt gctggtgcag ctcctttcgg gagacaaggc gcccgagggg cggcttcggg
     4321 gccgcgtgct gggcgtgctg aagaggaaga ggcacgagct ggcgtttgtg tgccgcatgg
     4381 acacgtggga cccgcgcatc atggtcccca tcaatggctc cgtgaccaag atcttcgtgg
     4441 ccgagctgaa ggacccatcg caggtcccca tctacagcct ccggaagggc cggctgcagc
     4501 gtgtggggct tgagaggctc accgccgagg cccggcacag ccggctcttc tgggtccaaa
     4561 ttgtcctgtg gcggcaaggc ttctactacc cgctgggcat cgtccgggag gtgctgcctg
     4621 aggccagcac ctgggagcag ggcctccgca tcctcggcct ggagtacagc ttgagggtgc
     4681 ccccgtcgga ccaggccacc atcaccaagg tgctgcagaa ataccacacg gagcttggcc
     4741 gggttgccgg ccgccgagag gactgccgcg ccttcttgac cttcactgtg gacccccagg
     4801 gcgcctgcaa cctcgatgat gccctcagtg tccgagacct gggtcccagg tgcgaggtgg
     4861 ctgtgcacat cactgatgtg gccagcttcg tgcccaggga cggggtgctg gacgtggagg
     4921 cgcgaaggca gggcgctgcg ttctatgccc ccggcaggga gccagtgccc atgctgccgg
     4981 ccagcctctg ccaggacgtc ctcagcctcc tgcctggccg ggaccgcctg gccatctccc
     5041 tgttcctcac catggagaag gccagtggcc agctgaagag cctgcgcttt gcaccctccg
     5101 tggtccagtc tgaccgccag ctgtcctacg aggaggcgga ggaggtgatc aggcagcacc
     5161 cgggtgccgg ccgtgagctg ccggcccgcc tggactccgt ggacgcctgc gtcgtggccg
     5221 cgtgctactt ctctcggctg ctgcgccggc accgcctgcg gtccgactgc ttctatgagc
     5281 agccggacga ggacggcacc ctgggcttcc gcgcggccca catcatggtg aaggagtaca
     5341 tgattcagtt taataggctc gtggctgagt tcctggtggg cagcgagtgc acgcggacgg
     5401 tcacgcctct gcggtggcag ccagcacccc gcagccagca gctcaaggcc ctgtgtgaga
     5461 agcatgggga ccgggtgccc ctgtcactgc acctcggcca ccacctgcac ggcggcgggg
     5521 gcagtccccc cgacacgcgg ctgcacctcc tggcctccct ctggaagcag gtccagtttg
     5581 ctgcccgcac ccaggactac gaacagatgg tggacttggt caccacggac gacatgcacc
     5641 cattcctggc tcctgcaggc cgcgacctcc gcaaggccct ggagcgctcg gcgttcggcc
     5701 gctgcgcccg gggccaccag cagcagggcg gccactactc gctgcaggtg gactggtaca
     5761 cgtgggccac ctcgcccatc cgcaggtacc tggacgtggt gttgcagcgg cagatcctgc
     5821 tggcgctggg ccatgggggc tctgcctact ctgccaggga catcgatggg ctctgccagg
     5881 ccttcagcct ccagcacgca cttgcccaga gctatcagcg gcgggcgcgc agcctgcacc
     5941 tggccgtgca gctcaaggcc cagcctctgg acaagctggg cttcgtggtg gacgtggagg
     6001 cgggctcccg ctgcttccgg ctgctcttcc ccagcaaccg ggagacgctg cctgacccct
     6061 gccccgtccc ctacggctcc ctgcagctgg ccgagcaccc ccacgccctg gcaggccggc
     6121 cgggcctgcg gctcctgtgg cggcgccgtg tctactcagc gcagggatcc agcccgcccc
     6181 tgccactgcc tggcactgtg ccggacccac acaccctggc cgtggagacg gccctgtgga
     6241 agcagctgct ggagctggtg gagctgcagc gctggccgga ggcggctgct ctcatccagg
     6301 agaagggcga ggcgtcccag cggcgggagc tggtgcaggt gcagcggagc cactgtggcc
     6361 atttcctgga ggtggcccgg gagctgggca gtggggacac cctgcaggtg cagctcggca
     6421 ccagcctgca gcacggcttc ctggtaccga gccctcagct ctggacggtg gcaccgggct
     6481 tcagcctctg cctggagcac gtggagcggc ccggagactg cttctcaggc cgtgtgtacc
     6541 gggccccgag ggaccggtac cgcgacgtgg atgagtacgc ctgcgtgtgg gaaccattct
     6601 gcgccctgga gtcggccacc ggcgcggttg ccgagaatga ctccgtcaca cttcagcacc
     6661 tgagtgtctc ctgggaggcg tcacggacgc cgcaggggca gctgcagggc gccttccgcc
     6721 tggaggccgc cttcctcgag gagaactgtg ccgacatcaa cttcagctgc tgctacctct
     6781 gcatccggct cgaggggctg ccggctccca cggccagccc acgccctggg cccagcagcc
     6841 tcggccctgg cctgaatgtt gaccccggca cgtatacctg ggtggcccac gggcagacgc
     6901 aggactggga ccaggagcgc cgggcagacc ggcaggaggc tcccagacgg gtgcacctct
     6961 tcgtccacca catgggcatg gagaaggttc cggaagaggt gctgaggccg ggcaccctgt
     7021 tcaccgttga gctgctgccc aagcagcttc ctgatctccg caaggaggaa gccgtgcgtg
     7081 gactagagga ggcgtccccg ctggtcacca gcatcgcact gggccggcct gtcccgcagc
     7141 ccctctgcag agtgatcccc agcaggttcc tggagcggca gacctacaac atccccggag
     7201 gccgccacaa gctgaacccc agccagaacg tggcggtcag ggaggctctg gagaagcctt
     7261 tcacggtcat tcagggccca ccaggtacag ggaagacgat cgtgggcctc cacatcgtat
     7321 tctggtttca taaatcaaac caggagcagg tgcagcccgg aggccccccc cgtggggaga
     7381 agcggctggg gggtccctgc atcttgtact gcggcccctc caacaagtcg gtggatgtcc
     7441 tggcaggact gctcctgaga aggatggagc tgaagcccct ccgtgtgtac agtgagcagg
     7501 ctgaggccag cgagttccca gtgccgcgtg tgggcagcag gaagctgctc aggaagagcc
     7561 cccgggaggg gaggccgaac cagagcctca ggagcatcac cctgcaccac cggatccggc
     7621 aggcccccaa cccttactcg tcggaaatca aggcctttga cacccggctg cagagagggg
     7681 agctcttctc cagggaggac ctggtctggt acaagaaggt cttgtgggag gctcggaagt
     7741 tcgagctgga ccggcatgag gtcatcctct gcacctgctc ctgtgcagcc tctgccagcc
     7801 tcaaaatcct ggacgtgagg cagatccttg ttgacgaggc aggcatggcc acggaacctg
     7861 aaaccctcat ccccctggtg cagttcccac aggccgagaa ggtggttctt ctcggagacc
     7921 acaagcagct gcggcctgtg gtcaagaatg agcggctgca aaacctgggt ctggaccggt
     7981 ctctgttcga gcggtaccac gaggacgcac atatgctgga cactcagtac cgcatgcatg
     8041 agggcatctg tgccttcccc tctgtggcgt tctacaagag caagctgaag acgtggcagg
     8101 gcctgaggag gccgcccagt gtcctgggcc acgctggcaa ggagagctgc cctgtcatct
     8161 ttggccacgt gcagggccac gagcggagcc tgctggtgtc cacggacgaa gggaatgaga
     8221 actccaaggc caacctggag gaggtggctg aggtggtccg tatcaccaag cagctgaccc
     8281 tggggaggac cgtagagccc caggacatcg ccgtcctcac gccctacaac gcgcaggcct
     8341 ctgagatcag caaggccctt cggcgagagg gcatcgccgg ggtggccgtg tcctccatca
     8401 ccaagagcca ggggagcgag tggcgctatg tgctggtgag caccgtccgc acctgtgcca
     8461 agagcgacct ggaccagcgg cccaccaaga gctggctcaa gaagtttctg ggcttcgttg
     8521 tggaccccaa ccaagtgaat gtggctgtca cgcgggccca ggaggggctc tgcctgatcg
     8581 gagaccacct ccttctgcgc tgctgccccc tctggcgtag cctcctggac ttctgcgagg
     8641 ctcagcagac cctcgtgcct gccggccagg tgcgcgtctg caggaggcca actatgcctt
     8701 ccggatccga ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg
     8761 atgacgatga caagtagtga gggcccgata tctcgacaat caacctctgg attacaaaat
     8821 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     8881 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     8941 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     9001 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     9061 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     9121 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     9181 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     9241 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     9301 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     9361 gatctccctt tgggccgcct ccccgcctgg aaacggggga ggctaactga aacacggaag
     9421 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     9481 gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
     9541 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
     9601 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
     9661 catagcagat ctgcgcagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     9721 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     9781 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     9841 tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac ggcacctcga
     9901 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     9961 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
    10021 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tggggatttc
    10081 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
    10141 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
    10201 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
    10261 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
    10321 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
    10381 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
    10441 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
    10501 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
    10561 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
    10621 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
    10681 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
    10741 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
    10801 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
    10861 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
    10921 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
    10981 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
    11041 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
    11101 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
    11161 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
    11221 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
    11281 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
    11341 ctctggggtt cgcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
    11401 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
    11461 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
    11521 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    11581 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    11641 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
    11701 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
    11761 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
    11821 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
    11881 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
    11941 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
    12001 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
    12061 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    12121 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    12181 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    12241 cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag
    12301 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    12361 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    12421 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    12481 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
    12541 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    12601 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    12661 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    12721 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    12781 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    12841 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    12901 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    12961 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    13021 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    13081 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    13141 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    13201 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    13261 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    13321 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    13381 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    13441 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    13501 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    13561 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    13621 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    13681 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    13741 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    13801 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
    13861 cccctatggt cgactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
    13921 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
    13981 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gg
//
