LOCUS V038256 10009 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038256 VERSION V038256 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..4668 /label="ADGRA3(NM_145290)" /note="ADGRA3(NM_145290)" /gene="ADGRA3" CDS 4675..4701 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4705..4731 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4735..4761 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" misc_feature 4785..5373 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 5439..5487 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5689..6117 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 6131..6460 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 6527..7321 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 7497..7618 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(7667..7683) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7691..7707 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7715..7745) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7760..7781 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8069..8657) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8828..9688) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9689..9793) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgga gccacccgga 721 cgccggcggg gccgcgcgca gccgccgctg ttgctgccgc tctcgctgtt agcgctgctc 781 gcgctgctgg gaggcggcgg cggcggcggc gccgcggcgc tgcccgccgg ctgcaagcac 841 gatgggcggc cccgaggggc tggcagggcg gcgggcgccg ccgagggcaa ggtggtgtgc 901 agcagcctgg aactcgcgca ggtcctgccc ccagatactc tgcccaaccg cacggtcacc 961 ctgattctga gtaacaataa gatatccgag ctgaagaatg gctcattttc tgggttaagt 1021 ctccttgaaa gattggacct ccgaaacaat cttattagta gtatagatcc aggtgccttc 1081 tggggactgt catctctaaa aagattggat ctgacaaaca atcgaatagg atgtctgaat 1141 gcagacatat ttcgaggact caccaatctg gttcggctaa acctttcggg gaatttgttt 1201 tcttcattat ctcaaggaac ttttgattat cttgcgtcat tacggtcttt ggaattccag 1261 actgagtatc ttttgtgtga ctgtaacata ctgtggatgc atcgctgggt aaaggagaag 1321 aacatcacgg tacgggatac caggtgtgtt tatcctaagt cactgcaggc ccaaccagtc 1381 acaggcgtga agcaggagct gttgacatgc gaccctccgc ttgaattgcc gtctttctac 1441 atgactccat ctcatcgcca agttgtgttt gaaggagaca gccttccttt ccagtgcatg 1501 gcttcatata ttgatcagga catgcaagtg ttgtggtatc aggatgggag aatagttgaa 1561 accgatgaat cgcaaggtat ttttgttgaa aagaacatga ttcacaactg ctccttgatt 1621 gcaagtgccc taaccatttc taatattcag gctggatcta ctggaaattg gggctgtcat 1681 gtccagacca aacgtgggaa taatacgagg actgtggata ttgtggtatt agagagttct 1741 gcacagtact gtcctccaga gagggtggta aacaacaaag gtgacttcag atggcccaga 1801 acattggcag gcattactgc atatctgcag tgtacgcgga acacccatgg cagtgggata 1861 tatcccggaa acccacagga tgagagaaaa gcttggcgca gatgtgatag aggtggcttt 1921 tgggcagatg atgattattc tcgctgtcag tatgcaaatg atgtcactag agttctttat 1981 atgtttaatc agatgcccct caatcttacc aatgccgtgg caacagctcg acagttactg 2041 gcttacactg tggaagcagc caacttttct gacaaaatgg atgttatatt tgtggcagaa 2101 atgattgaaa aatttggaag atttaccaag gaggaaaaat caaaagagct aggtgacgtg 2161 atggttgaca ttgcaagtaa catcatgttg gctgatgaac gtgtcctgtg gctggcgcag 2221 agggaagcta aagcctgcag taggattgtg cagtgtcttc agcgcattgc tacctaccgg 2281 ctagccggtg gagctcacgt ttattcaaca tattcaccca atattgctct ggaagcttat 2341 gtcatcaagt ctactggctt cacggggatg acctgtaccg tgttccagaa agtggcagcc 2401 tctgatcgta caggactttc ggattatggg aggcgggatc cagagggaaa cctggataag 2461 cagctgagct ttaagtgcaa tgtttcaaat acattttcga gtctggcact aaagaatact 2521 attgtggagg cttctattca gcttcctcct tcccttttct caccaaagca aaaaagagaa 2581 ctcagaccaa ctgatgactc tctttacaag cttcaactca ttgcattccg caatggaaag 2641 ctttttccag ccactggaaa ttcaacaaat ttggctgatg atggaaaacg acgtactgtg 2701 gttacccctg tgattctcac caaaatagat ggtgtgaatg tagataccca ccacatccct 2761 gttaatgtga cactgcgtcg aattgcacat ggagcagatg ctgttgcagc ccggtgggat 2821 ttcgatttgc tgaacggaca aggaggctgg aagtcagatg ggtgccatat actctattca 2881 gatgaaaata tcactacgat tcagtgctac tcccttagta actatgcagt tttaatggat 2941 ttgacgggat ctgaactata cacccaggcg gccagcctcc tgcatcctgt ggtttatact 3001 accgctatca ttctcctctt atgtctctta gccgtcattg tcagttacat ataccatcac 3061 agtttgatta gaatcagcct caagagctgg cacatgcttg tgaacttgtg ctttcatatt 3121 ttcctaacct gtgtggtctt tgtgggagga ataacccaga ctaggaatgc cagcatctgc 3181 caagcagttg ggataattct tcactattcc acccttgcca cagtactatg ggtaggagtg 3241 acagctcgaa atatctacaa acaagtcact aaaaaagcta aaagatgcca ggatcctgat 3301 gaaccaccac ctccaccaag accaatgctc agattttacc tgattggtgg tggtatcccc 3361 atcattgttt gcggcataac tgcagcagcg aacattaaga attacggcag tcggccaaac 3421 gcaccctatt gctggatggc atgggaaccc tccttgggag ccttctatgg gccagccagc 3481 ttcatcactt ttgtaaactg catgtacttt ctgagcatat ttattcagtt gaaaagacac 3541 cctgagcgca aatatgagct taaggagccc acggaggagc aacagagatt ggcagccaat 3601 gaaaatggcg aaataaatca tcaggattca atgtctttgt ctctgatttc tacatcagcc 3661 ttggaaaatg agcacacttt tcattctcag ctcttggggg ccagccttac tttgctctta 3721 tatgttgcac tgtggatgtt tggggctttg gctgtttctt tgtattaccc tttggacttg 3781 gtttttagct tcgtttttgg agccacaagt ttaagcttca gtgcgttctt cgtggtccac 3841 cattgtgtta atagggagga tgttagactt gcgtggatca tgacttgctg cccaggacgg 3901 agctcgtatt cagtgcaagt caacgtccag ccccccaact ctaatgggac gaatggagag 3961 gcacccaaat gccccaatag cagtgcggag tcttcatgca caaacaaaag tgcttcaagc 4021 ttcaaaaatt cctcccaggg ctgcaaatta acaaacttgc aggcggctgc agctcagtgc 4081 catgccaatt ctttaccttt gaactccacc cctcagcttg ataatagtct gacagaacat 4141 tcaatggaca atgatattaa aatgcacgtg gcgcctttag aagttcagtt tcgaacaaat 4201 gtgcactcaa gccgccacca taaaaacaga agtaaaggac accgggcaag ccgactcaca 4261 gtcctgagag aatatgccta cgatgtccca acgagcgtgg aaggaagcgt gcagaacggc 4321 ttacctaaaa gccggctggg caataacgaa ggacactcga ggagccgaag agcttattta 4381 gcctacagag agagacagta caacccaccc cagcaagaca gcagcgatgc ttgtagcaca 4441 cttcccaaaa gtagcagaaa ttttgaaaag ccagtttcaa ccactagtaa aaaagatgcg 4501 ttaaggaagc cagctgtggt tgaacttgaa aatcagcaaa aatcttatgg cctcaacttg 4561 gccattcaga atggaccaat taaaagcaat gggcaggagg gacccttgct cggtaccgat 4621 agcactggca atgttaggac tggattatgg aaacacgaaa ctactgtgct cgagtaccca 4681 tacgacgtac ctgattatgc aacttatcct tatgatgtac ctgattatgc tagctatcct 4741 tatgatgtcc cagactacgc atagtgaggg cccgatatct cgacaatcaa cctctggatt 4801 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 4861 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 4921 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 4981 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 5041 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 5101 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 5161 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct 5221 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 5281 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga 5341 cgagtcggat ctccctttgg gccgcctccc cgcctggaaa cgggggaggc taactgaaac 5401 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa 5461 aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact 5521 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc 5581 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag 5641 gccctgccat agcagatctg cgcagctggg gctctagggg gtatccccac gcgccctgta 5701 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 5761 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 5821 ttccccgtca agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc 5881 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat 5941 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 6001 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg 6061 ggatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat 6121 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag 6181 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 6241 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 6301 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 6361 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa 6421 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat 6481 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga 6541 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc 6601 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc 6661 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc 6721 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac 6781 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc 6841 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac 6901 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg 6961 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 7021 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt 7081 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg 7141 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac 7201 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg 7261 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg 7321 agcgggactc tggggttcgc gaaatgaccg accaagcgac gcccaacctg ccatcacgag 7381 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg 7441 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact 7501 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 7561 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 7621 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc 7681 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 7741 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 7801 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 7861 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 7921 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 7981 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 8041 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 8101 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 8161 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 8221 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt 8281 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 8341 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 8401 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 8461 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 8521 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 8581 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 8641 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 8701 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 8761 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 8821 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 8881 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 8941 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 9001 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 9061 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 9121 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 9181 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 9241 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 9301 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 9361 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 9421 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 9481 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 9541 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 9601 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 9661 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 9721 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 9781 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc 9841 tcccgatccc ctatggtcga ctctcagtac aatctgctct gatgccgcat agttaagcca 9901 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc 9961 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttagg //