LOCUS V019766 8894 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V019766 VERSION V019766 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 683..3574 /label="ADAM22(NM_001324418)" /note="ADAM22(NM_001324418)" /gene="ADAM22" CDS 3581..3646 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" misc_feature 3670..4258 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 4324..4372 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 4574..5002 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 5016..5345 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5412..6206 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 6382..6503 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(6552..6568) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6576..6592 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6600..6630) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6645..6666 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6954..7542) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7713..8573) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8574..8678) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccgcca ccatgcaggc ggcagtggct gtgtccgtgc ccttcttgct 721 gctctgtgtc ctggggacct gccctccggc gcgctgcggc caggcaggag acgcctcatt 781 gatggagcta gagaagagga aggaaaaccg cttcgtggag cgccagagca tcgtgccact 841 gcgcctcatc taccgctcgg gcggcgaaga cgaaagtcgg cacgacgcgc tcgacacgcg 901 ggtgcggggc gacctcggtg gcccgcagtt gactcatgtt gaccaagcaa gcttccaggt 961 tgatgccttt ggaacgtcat tcattctcga tgtcgtgcta aatcatgatt tgctgtcctc 1021 tgaatacata gagagacaca ttgaacatgg aggcaagact gtggaagtta aaggaggaga 1081 gcactgttac taccagggcc atatccgagg aaaccctgac tcatttgttg cattgtcaac 1141 atgccacgga cttcatggga tgttctatga cgggaaccac acatatctca ttgagccaga 1201 agaaaatgac actactcaag aggatttcca ttttcattca gtttacaaat ccagactgtt 1261 tgaattttcc ttggatgatc ttccatctga atttcagcaa gtaaacatta ctccatcaaa 1321 atttattttg aagccaagac caaaaaggag taaacggcag cttcgtcgat atcctcgtaa 1381 tgtagaagaa gaaaccaaat acattgaact gatgattgtg aatgatcacc ttatgtttaa 1441 aaaacatcgg ctttccgttg tacataccaa tacctatgcg aaatctgtgg tgaacatggc 1501 agatttaata tataaagacc aacttaagac caggatagta ttggttgcta tggaaacctg 1561 ggcgactgac aacaagtttg ccatatctga aaatccattg atcaccctac gtgagtttat 1621 gaaatacagg agggatttta tcaaagagaa aagtgatgca gttcaccttt tttcgggaag 1681 tcaatttgag agtagccgga gcggggcagc ttatattggt gggatttgct cgttgctgaa 1741 aggaggaggc gtgaatgaat ttgggaaaac tgatttaatg gctgttacac ttgcccagtc 1801 attagcccat aatattggta ttatctcaga caaaagaaag ttagcaagtg gtgaatgtaa 1861 atgcgaggac acgtggtccg ggtgcataat gggagacact ggctattatc ttcctaaaaa 1921 gttcacccag tgtaatattg aagagtatca tgacttcctg aatagtggag gtggtgcctg 1981 ccttttcaac aaaccttcta agcttcttga tcctcctgag tgtggcaatg gcttcattga 2041 aactggagag gagtgtgatt gtggaacccc ggccgaatgt gtccttgaag gagcagagtg 2101 ttgtaagaaa tgcaccttga ctcaagactc tcaatgcagt gacggtcttt gctgtaaaaa 2161 gtgcaagttt cagcctatgg gcactgtgtg ccgagaagca gtaaatgatt gtgatattcg 2221 tgaaacgtgc tcaggaaatt caagccagtg tgcccctaat attcataaaa tggatggata 2281 ttcatgtgat ggtgttcagg gaatttgctt tggaggaaga tgcaaaacca gagatagaca 2341 atgcaaatac atttgggggc aaaaggtgac agcatcagac aaatattgct atgagaaact 2401 gaatattgaa gggacggaga agggtaactg tgggaaagac aaagacacat ggatacagtg 2461 caacaaacgg gatgtgcttt gtggttacct tttgtgtacc aatattggca atatcccaag 2521 gcttggagaa ctcgatggtg aaatcacatc tactttagtt gtgcagcaag gaagaacatt 2581 aaactgcagt ggtgggcatg ttaagcttga agaagatgta gatcttggct atgtggaaga 2641 tgggacacct tgtggtcccc aaatgatgtg cttagaacac aggtgtcttc ctgtggcttc 2701 tttcaacttt agtacttgct tgagcagtaa agaaggcact atttgctcag gaaatggagt 2761 ttgcagtaat gagctgaagt gtgtgtgtaa cagacactgg ataggttctg attgcaacac 2821 ttacttccct cacaatgatg atgcaaagac tggtatcact ctgtctggca atggtgttgc 2881 tggcaccaat atcataatag gcataattgc tggcaccatt ttagtgctgg ccctcatatt 2941 aggaataact gcgtggggtt ataaaaacta tcgagaacag agacagttac cccagggaga 3001 ttatgtaaaa aagcctggag atggtgactc tttttatagc gacattcctc ccggagtcag 3061 cacaaactca gcatctagtt ctaagaagag gtctgctttt ctgtcgcatt ttcagatttc 3121 tacctgttcc atcacacatt attccattag tcagaacatt tcattatttt gcagcaggtc 3181 aaatgggctc tctcattctt ggagtgaaag gattccagac acaaaacata tttcagacat 3241 ctgtgaaaat gggcgacctc gaagtaactc ttggcaaggt aacctgggag gcaacaaaaa 3301 gaaaatcaga ggcaaaagat ttagacctcg gtctaattca actgagtatt taaacccatg 3361 gttcaaaaga gactataatg tagctaagtg ggtagaagat gtgaataaaa acactgaagg 3421 accatacttt aggactttat ctcctgccaa gtctccttct tcatcaactg ggtctattgc 3481 ctccagcaga aaataccctt acccaatgcc tccacttcct gatgaggaca agaaagtgaa 3541 ccgacaaagt gccaggctat gggagacatc cattctcgag gactacaaag accatgacgg 3601 tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtagt gagggcccga 3661 tatctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac 3721 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt 3781 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat 3841 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca 3901 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc 3961 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg 4021 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca 4081 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct 4141 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt 4201 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct 4261 ggaaacgggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta 4321 tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc 4381 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa 4441 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg 4501 ctcgcagcca acgtcggggc ggcaggccct gccatagcag atctgcgcag ctggggctct 4561 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 4621 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 4681 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta 4741 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 4801 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg 4861 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat 4921 tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt 4981 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt 5041 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 5101 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 5161 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 5221 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 5281 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt 5341 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag 5401 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg 5461 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt 5521 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc 5581 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt 5641 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag 5701 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg 5761 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag 5821 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg 5881 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc 5941 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca 6001 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc 6061 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg 6121 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct 6181 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa 6241 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg 6301 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg 6361 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc 6421 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 6481 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg 6541 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 6601 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 6661 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 6721 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 6781 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 6841 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 6901 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 6961 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 7021 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 7081 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 7141 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 7201 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 7261 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 7321 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 7381 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 7441 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 7501 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 7561 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 7621 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 7681 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 7741 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 7801 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 7861 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 7921 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 7981 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 8041 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 8101 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 8161 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 8221 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 8281 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 8341 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 8401 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 8461 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 8521 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 8581 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 8641 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 8701 cctgacgtcg acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct 8761 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg 8821 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga 8881 agaatctgct tagg //