LOCUS       .                       8457 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        96..200
                     /label="AmpR promoter"
     CDS             275..1462
                     /label="TcR"
                     /note="tetracycline efflux protein"
     rep_origin      1739..2327
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2615..2636
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2651..2681
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2689..2705
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     2713..2729
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(4907..4923)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      5273..5359
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      5451..5478
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     oriT            5899..6008
                     /label="oriT"
                     /note="incP origin of transfer"
     promoter        6382..6827
                     /label="sacB promoter"
                     /note="sacB promoter and control region"
     CDS             6828..8246
                     /label="SacB"
                     /note="secreted levansucrase that renders bacterial growth
                     sensitive to sucrose"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aaccgattag ctttaatgcg gtagtttatc
      241 acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt
      301 catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc cggtactgcc
      361 gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg gcgtgctgct
      421 agcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac tgtccgaccg
      481 ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg actacgcgat
      541 catggcgacc acacccgtcc tgtggattct ctacgccgga cgcatcgtgg ccggcatcac
      601 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
      661 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
      721 ggccggggga ctgttgggcg ccatctcctt acatgcacca ttccttgcgg cggcggtgct
      781 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
      841 ccgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat
      901 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
      961 ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg
     1021 cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc
     1081 cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct
     1141 gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct
     1201 tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga
     1261 tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat
     1321 cactggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt
     1381 ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc
     1441 atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc
     1501 actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct
     1561 tgcatcgatt gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1621 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1681 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1741 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1801 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1861 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1921 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1981 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2041 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2101 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2161 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2221 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2281 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2341 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2401 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2461 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2521 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     2581 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     2641 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     2701 aacaatttca cacaggaaac agctatgaca tgattacgaa ttcgtggatc gcacgcaact
     2761 gatcagcaat gtgcgcagca acctggcggg actggggcgc ggcagcctcg gcgtgccggt
     2821 cctgctgctg gccatgctgg cgatgatgac cctgccgatc ccgccattcc tgctggacgt
     2881 gctgttcacc ttcaacatcg ccctgtccat cgtcgtcctg ctggtctgcg tgtacgccct
     2941 gcggccgctg gacttcgccg tgttcccgac catcctgctg gtggcgaccc tgttgcgcct
     3001 ggcgctgaac gttgcctcga cccgcgtggt gctgctccac ggccacgacg gccatggcgc
     3061 cgcaggcaag gtgatccagg ccttcggcga agtggtgatc ggcggcaact acgtggtcgg
     3121 ggtggtggtg ttcgcgatcc tcatgatcat caacttcgtg gtggtgacca agggcgccgg
     3181 gcggatttcc gaagtcagcg cgcgcttcac cctggatgcc atgcccggca agcagatggc
     3241 catcgacgcc gacctcaacg ccggcctgat cgagcaggag gaagccaaga agcgccgcag
     3301 cgaggtggcc caggaggccg acttctacgg ttcgatggac ggtgccagca agttcgtccg
     3361 cggcgacgcc atcgccggcc tgctgattct cttcatcaac ctgatcggcg gcgtcgccat
     3421 cggcatgatc cagcattcga tgtccttcgg cgatgccggc aaggtctacg ccctgctgac
     3481 catcggtgac ggcctggtgg cacaactgcc gtcgctgctg ctgtcgaccg cggcggcgat
     3541 catggtgacg cgggtctcca gttccgagga catgggccag caggtcaacc ggcagatgtt
     3601 cgcctcgccc aaggcgctgg cgatttccgc ggcgatcctg atcgccatgg gcgccgtgcc
     3661 gggcatgccg catgtttcct tcatcggcct cggcgccctg gccggcgctg ccgcctactg
     3721 gatctggcac cggcagaaca aggccaagca ggtcgccgag caggaggtgc agcgccagca
     3781 ggagctgctg ccggcgcagc gcgcccagga ggtcaaggaa ctgggttggg acgacgtcac
     3841 cccggtggac atggtcggcc tggaagtcgg ctaccggctg atcccgctgg tcgaccgcaa
     3901 ccagggcggg caactgctgg cgcggatcaa gggcgtacgc aagaagctgt cccaggagct
     3961 gggcttcctg atgccctcgg tgcacatccg cgacaacctc gacctgctgc ccaacgccta
     4021 ccgtctgacc ctgatggggg tcagcgtggc cgaagcggag atctatcccg atcgcgagct
     4081 ggcgatcaac cccggccagg tattcggcag cctcaacggc atcgccggca aggacccggc
     4141 cttcggcctg gaggcggtat ggatcgagcc cggccagcgc gaccaggcgc agtcgctggg
     4201 ctacaccgtg gtggatgcga gcacagtggt cgccactcac ctgaatcagg tgctgcacaa
     4261 gcacgcccac gagctgctgg gccatgaaga agtgcaacag ctcctgcaat tgctcgcgcg
     4321 cagctcgccg aagctggcgg aagagctggt gccggggctg atttccctgt ccaccctgct
     4381 caaggtcctg caggcgctgc tccaggagca ggtgccggtg cgcgacatcc gtaccatcgc
     4441 cgaggccatc gccaacgtcg ctccacggag tcaagatccc ggcgcgatgg tcgccgcggt
     4501 tcgcgtcgcg ttgtcccgcg caatcgtgca aagcattgtc gggcttgagc cggagctgcc
     4561 tgtgatcacg ctcgagccga ggttggaaca gattttgctg aacagcatgc agaaggccgg
     4621 acaaggctcc gaggacggca tgttgctgga gccggggatg gccgagaagc tgcaacgctc
     4681 gctgatcgag agcgcgcaac gccaggagat gctcggtaag ccggcgatcc tgctggttgc
     4741 cggaccgatc cgctcgatga tgtcgcgctt cgcgcggatg gcggtgccca gcatgcatgt
     4801 gctggcctac caggaaatcc cggacaacaa gcaagtcacc atcgtcgcga cggtgggaca
     4861 gaactgagga tcctctagag tcgacctgca ggcatgcaag cttggcactg gccgtcgttt
     4921 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     4981 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     5041 tgcgcagcct gaatggcgaa tggcgagctt ggctgttttg gcggatgaga gaagattttc
     5101 agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc
     5161 ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc
     5221 gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa
     5281 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     5341 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg
     5401 agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat
     5461 cctgacggat ggcctttttg cgtttctaca aactcttttt gtttattttt ctaaatacat
     5521 tcaaatatgc gcctgatgcg gtattttctc cttacgcata tcgacatccg ccctcaccgc
     5581 caggaacgca accgcagcct catcacgccg gcgcttcttg gccgcgcggg attcaaccca
     5641 ctcggccagc tcgtcggtgt agctctttgg catcgtctct cgcctgtccc ctcagttcag
     5701 taatttcctg catttgcctg tttccagtcg gtagatattc cacaaaacag cagggaagca
     5761 gcgcttttcc gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc
     5821 atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg
     5881 tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc
     5941 cttcttcact gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg
     6001 ctggccggct accgccggcg taacagatga gggcaagcgg atggctgatg aaaccaagcc
     6061 aaccaggaag ggcagcccac ctatcaaggt gtactgcctt ccagacgaac gaagagcgat
     6121 tgaggaaaag gcggcggcgg ccggcatgag cctgtcggcc tacctgctgg ccgtcggcca
     6181 gggctacaaa atcacgggcg tcgtggacta tgagcacgtc cgcgagctgg cccgcatcaa
     6241 tggcgacctg ggccgcctgg gcggcctgct gaaactctgg ctcaccgacg acccgcgcac
     6301 ggcgcggttc ggtgatgcca cgatcctcgc cctgctggcg aagatcgact ctagctagag
     6361 gatcgatcct ttttaaccca tcacatatac ctgccgttca ctattattta gtgaaatgag
     6421 atattatgat attttctgaa ttgtgattaa aaaggcaact ttatgcccat gcaacagaaa
     6481 ctataaaaaa tacagagaat gaaaagaaac agatagattt tttagttctt taggcccgta
     6541 gtctgcaaat ccttttatga ttttctatca aacaaaagag gaaaatagac cagttgcaat
     6601 ccaaacgaga gtctaataga atgaggtcga aaagtaaatc gcgcgggttt gttactgata
     6661 aagcaggcaa gacctaaaat gtgtaaaggg caaagtgtat actttggcgt caccccttac
     6721 atattttagg tcttttttta ttgtgcgtaa ctaacttgcc atcttcaaac aggagggctg
     6781 gaagaagcag accgctaaca cagtacataa aaaaggagac atgaacgatg aacatcaaaa
     6841 agtttgcaaa acaagcaaca gtattaacct ttactaccgc actgctggca ggaggcgcaa
     6901 ctcaagcgtt tgcgaaagaa acgaaccaaa agccatataa ggaaacatac ggcatttccc
     6961 atattacacg ccatgatatg ctgcaaatcc ctgaacagca aaaaaatgaa aaatatcaag
     7021 ttcctgagtt cgattcgtcc acaattaaaa atatctcttc tgcaaaaggc ctggacgttt
     7081 gggacagctg gccattacaa aacgctgacg gcactgtcgc aaactatcac ggctaccaca
     7141 tcgtctttgc attagccgga gatcctaaaa atgcggatga cacatcgatt tacatgttct
     7201 atcaaaaagt cggcgaaact tctattgaca gctggaaaaa cgctggccgc gtctttaaag
     7261 acagcgacaa attcgatgca aatgattcta tcctaaaaga ccaaacacaa gaatggtcag
     7321 gttcagccac atttacatct gacggaaaaa tccgtttatt ctacactgat ttctccggta
     7381 aacattacgg caaacaaaca ctgacaactg cacaagttaa cgtatcagca tcagacagct
     7441 ctttgaacat caacggtgta gaggattata aatcaatctt tgacggtgac ggaaaaacgt
     7501 atcaaaatgt acagcagttc atcgatgaag gcaactacag ctcaggcgac aaccatacgc
     7561 tgagagatcc tcactacgta gaagataaag gccacaaata cttagtattt gaagcaaaca
     7621 ctggaactga agatggctac caaggcgaag aatctttatt taacaaagca tactatggca
     7681 aaagcacatc attcttccgt caagaaagtc aaaaacttct gcaaagcgat aaaaaacgca
     7741 cggctgagtt agcaaacggc gctctcggta tgattgagct aaacgatgat tacacactga
     7801 aaaaagtgat gaaaccgctg attgcatcta acacagtaac agatgaaatt gaacgcgcga
     7861 acgtctttaa aatgaacggc aaatggtatc tgttcactga ctcccgcgga tcaaaaatga
     7921 cgattgacgg cattacgtct aacgatattt acatgcttgg ttatgtttct aattctttaa
     7981 ctggcccata caagccgctg aacaaaactg gccttgtgtt aaaaatggat cttgatccta
     8041 acgatgtaac ctttacttac tcacacttcg ctgtacctca agcgaaagga aacaatgtcg
     8101 tgattacaag ctatatgaca aacagaggat tctacgcaga caaacaatca acgtttgcgc
     8161 ctagcttcct gctgaacatc aaaggcaaga aaacatctgt tgtcaaagac agcatccttg
     8221 aacaaggaca attaacagtt aacaaataaa aacgcaaaag aaaatgccga ttatggtgca
     8281 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     8341 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     8401 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcga
//
