LOCUS       62056_10390        3747 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(834..850)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(858..874)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(882..912)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(927..948)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1236..1824)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1998..2855)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIRYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2856..2960)
                     /label=AmpR promoter
ORIGIN
        1 atttaggtga cactatagaa ctcacctatc tccccaacac ctaataacat tcaatcactc
       61 tttccactaa ccacctatct acatcaccaa gatatcacta gttctcgagc tcggtacctg
      121 tccgcggtcg cgacgtacgc gggcggccgc cataaattgg atccatatat agggcccggg
      181 ttataattac ctcaggtcga cgtcccatgg ttttgtatag aatttacggc tagcgccgga
      241 tgcgacgccg gtcgcgtctt atccggcctt cctatatcag gcggtgttta agacgccgcc
      301 gcttcgccca aatccttatg ccggttcgac gactggacaa aatactgttt atcttcccag
      361 cgcaggcagg ttaatgtacc accccagcag cagccggtat ccagcgcgta tataccttcc
      421 ggcgtacctt tgccctccag cgatgcccag tgaccaaagg cgatgctgta ttcttcagcg
      481 acagggccag gaatcgcaaa ccacggtttc agtggggcag gggcctcttc cggcgattct
      541 tactagctag tatgcatagg tgctgaaata taaagtttgt gtttctaaaa cacacgtggt
      601 acgtacgata acgtacagtg tttttccctc cacttaaatc gaagggtagt gtcttggagc
      661 gcgcggagta aacatatatg gttcatatat gtccgtaggc acgtaaaaaa agcgagggat
      721 tcgaattccc ccggaacccc cggttggggc ccacgcctcg atcgagcaaa aaaaaaaaaa
      781 aagaaaaaaa aaaaaaaaaa aagctttccc gcggccagct tggcgtaatc atggtcatag
      841 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      901 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      961 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     1021 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcact cactgactcg
     1081 ctgcgctcgg tcgctcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     1141 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     1201 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     1261 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     1321 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     1381 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1441 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1501 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1561 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     1621 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     1681 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     1741 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1801 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1861 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1921 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1981 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     2041 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     2101 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     2161 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     2221 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     2281 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     2341 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     2401 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     2461 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     2521 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagcgtatg
     2581 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     2641 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     2701 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     2761 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     2821 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     2881 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2941 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     3001 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
     3061 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     3121 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     3181 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     3241 tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc
     3301 gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc
     3361 ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg
     3421 agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc
     3481 gccggtgatg ccggccacga tgcgtccggc gtagaggatc tggctagcga tgaccctgct
     3541 gattggttcg ctgaccattt ccgggtgcgg gacggcgtta ccagaaactc agaaggttcg
     3601 tccaaccaaa ccgactctgg cggcagttta cgagagagat gatagggtct gcttcagtaa
     3661 gccagatgct acacaattag gcttgtacat actgtcgtta gaacgcggct acaattaata
     3721 cataacctta tgtatcatac acatacg
//
