LOCUS       62056_8600       10527 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10527)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1105..1761)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(1762..1864)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     RBS             2071..2093
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2101..2814
                     /codon_start=1
                     /label=GFPuv
                     /note="GFP variant optimized for excitation by UV light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     promoter        complement(2914..2944)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2959..2980)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3647..4495)
                     /codon_start=1
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="VVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(4485..5321)
                     /codon_start=1
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(5835..6803)
                     /codon_start=1
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            complement(8042..8129)
                     /direction=LEFT
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      8471..8865
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             complement(9682..10494)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc
       61 tcgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt
      121 aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga
      181 gattttgaga cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc
      241 agatcacgca tcttcccgac aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca
      301 ccaactggtc cacctacaac aaagctctca tcaaccgtgg ctccctcact ttctggctgg
      361 atgatggggc gattcaggcc tggtatgagt cagcaacacc ttcttcacga ggcagacctc
      421 agcgctattc tgaccttgcc atcacgactg tgctggtcat taaacgcgta ttcaggctga
      481 ccctgcgcgc tgcgcagggc tttattgatt ccatttttac actgatgaat gttccgttgc
      541 gctgcccgga ttacagctgc gtttcctgtt tggggtcgtt tgcgggaagg ggcggaatcc
      601 tacgctaagg ctttggccag cgatattctc cggtgagatt gatgtgttcc caggggatag
      661 gagaagtcgc ttgatatcta gtatgacgtc tgtcgcacct gcttgatcgc ggccgcgata
      721 gctagatcgc gttgctcctc ttctccatcc gcgttccaag ctgcggaaag gcacccataa
      781 gcgtacgcct ggtcgagcag gcgacgcgga tcgacgtcca gcgcacgaga gaatgcgtcc
      841 gccatctgtg caatgcgtct aggatcgaga caaaggtcgt ctctgtcagc cggatcgtag
      901 aacatattgg cggcgccaaa gcccacttca ccgaccagac attcgccatt caggctgcgc
      961 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     1021 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     1081 aaaacgacgg ccagtgaatt cttacgcccc gccctgccac tcatcgcagt actgttgtaa
     1141 ttcattaagc attctgccga catggaagcc atcacagacg gcatgatgaa cctgaatcgc
     1201 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
     1261 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
     1321 ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
     1381 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
     1441 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
     1501 actatcccat atcaccagct caccgtcttt cattgccata cggaattccg gatgagcatt
     1561 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
     1621 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
     1681 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
     1741 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
     1801 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccg
     1861 atcaggtacc cggggatcta gaggatctca atgaatattg gttgacacgg gcgtataaga
     1921 catgttatac tgttgaataa caagctttca attctctatt ttgagaagaa ttaatgtgct
     1981 tgggagtccc tgatgattaa attaaataaa ccaagatttt accatgactg caattttaga
     2041 gagacggggg atcgatcctc tagaaataat tttgtttaac tttaagaagg agatatacat
     2101 atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
     2161 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
     2221 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
     2281 gtcactactt tctcttatgg tgttcaatgc ttttcccgtt atccggatca tatgaaacgg
     2341 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac tatatctttc
     2401 aaagatgacg ggaactacaa gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt
     2461 aatcgtatcg agttaaaagg tattgatttt aaagaagatg gaaacattct cggacacaaa
     2521 ctcgagtaca actataactc acacaatgta tacatcacgg cagacaaaca aaagaatgga
     2581 atcaaagcta acttcaaaat tcgccacaac attgaagatg gatcagttca actagcagac
     2641 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     2701 ctgtcgacac aatctgccct ttcgaaagat cccaacgaaa agcgtgacca catggtcctt
     2761 cttgagtttg taactgctgc tgggattaca catggcatgg atgagctcta caaataagga
     2821 tcctctagag tcgacctgca ggcatgcaag cttggcgtaa tcatggtcat agctgtttcc
     2881 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     2941 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     3001 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     3061 gagaggcggt ttgcgtattg ggccacgctt cccgaaggga gaaaggcgga caggtatccg
     3121 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     3181 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3241 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3301 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3361 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     3421 agcgagtcag tgagcgagga agcggaaaga agagcataca tctggaagca aagccaggaa
     3481 agcggcctat ggagctgtgc ggcagcgctc agtaggcaat ttttcaaaat attgttaagc
     3541 cttttctgag catggtattt ttcatggtat taccaattag caggaaaata agccattgaa
     3601 tataaaagat aaaaatgtct tgtttacaat agagtggggg gggtcagcct gccgccttgg
     3661 gccgggtgat gtcgtacttg cccgccgcga actcggttac cgtccagccc agcgcgacca
     3721 gctccggcaa cgcctcgcgc acccgctggc ggcgcttgcg catggtcgaa ccactggcct
     3781 ctgacggcca gacatagccg cacaaggtat ctatggaagc cttgccggtt ttgccggggt
     3841 cgatccagcc acacagccgc tggtgcagca ggcgggcggt ttcgctgtcc agcgcccgca
     3901 cctcgtccat gctgatgcgc acatgctggc cgccacccat gacggcctgc gcgatcaagg
     3961 ggttcagggc cacgtacagg cgcccgtccg cctcgtcgct ggcgtactcc gacagcagcc
     4021 gaaacccctg ccgcttgcgg ccattctggg cgatgatgga taccttccaa aggcgctcga
     4081 tgcagtcctg tatgtgcttg agcgccccac cactatcgac ctctgccccg atttcctttg
     4141 ccagcgcccg atagctacct ttgaccacca tggcatcagc ggtgacggcc tcccacttgg
     4201 gttccaggaa cagccggagc tgccgtccgc cttcggtctt gggttccggg ccaagcacta
     4261 ggccattagg cccagccatg gccaccagcc cttgcaggat gcgcagatca tcagcgccca
     4321 gcggctccgg gccgctgaac tcgatccgct tgccgtcgcc gtagtcatac gtcacgtcca
     4381 gcttgctgcg cttgcgctcg ccccgcttga gggcacggaa caggccgggg gccagacagt
     4441 gcgccgggtc gtgccggacg tggctgaggc tgtgcttgtt cttaggcttc accacggggc
     4501 acccccttgc tcttgcgctg cctctccagc acggcgggct tgagcacccc gccgtcatgc
     4561 cgcctgaacc accgatcagc gaacggtgcg ccatagttgg ccttgctcac accgaagcgg
     4621 acgaagaacc ggcgctggtc gtcgtccaca ccccattcct cggcctcggc gctggtcatg
     4681 ctcgacaggt aggactgcca gcggatgtta tcgaccagta ccgagctgcc ccggctggcc
     4741 tgctgctggt cgcctgcgcc catcatggcc gcgcccttgc tggcatggtg caggaacacg
     4801 atagagcacc cggtatcggc ggcgatggcc tccatgcgac cgatgacctg ggccatgggg
     4861 ccgctggcgt tttcttcctc gatgtggaac cggcgcagcg tgtccagcac catcaggcgg
     4921 cggccctcgg cggcgcgctt gaggccgtcg aaccactccg gggccatgat gttgggcagg
     4981 ctgccgatca gcggctggat cagcaggccg tcagccacgg cttgccgttc ctcggcgctg
     5041 aggtgcgccc caagggcgtg caggcggtga tgaatggcgg tgggcgggtc ttcggcgggc
     5101 aggtagatca ccgggccggt gggcagttcg cccacctcca gcagatccgg cccgcctgca
     5161 atctgtgcgg ccagttgcag ggccagcatg gatttaccgg caccaccggg cgacaccagc
     5221 gccccgaccg taccggccac catgttgggc aaaacgtagt ccagcggtgg cggcgctgct
     5281 gcgaacgcct ccagaatatt gataggctta tgggtagcca ttgattgcct cctttgcagg
     5341 cagttggtgg ttaggcgctg gcggggtcac tacccccgcc ctgcgccgct ctgagttctt
     5401 ccaggcactc gcgcagcgcc tcgtattcgt cgtcggtcag ccagaacttg cgctgacgca
     5461 tccctttggc cttcatgcgc tcggcatatc gcgcttggcg tacagcgtca gggctggcca
     5521 gcaggtcgcc ggtctgcttg tccttttggt ctttcatatc agtcaccgag aaacttgccg
     5581 gggccgaaag gcttgtcttc gcggaacaag gacaaggtgc agccgtcaag gttaaggctg
     5641 gccatatcag cgactgaaaa gcggccagcc tcggccttgt ttgacgtata accaaagcca
     5701 ccgggcaacc aatagccctt gtcacttttg atcaggtaga ccgaccctga agcgcttttt
     5761 tcgtattcca taaaaccccc ttctgtgcgt gagtactcat agtataacag gcgtgagtac
     5821 caacgcaagc actacatgct gaaatctggc ccgcccctgt ccatgcctcg ctggcggggt
     5881 gccggtgccc gtgccagctc ggcccgcgca agctggacgc tgggcagacc catgaccttg
     5941 ctgacggtgc gctcgatgta atccgcttcg tggccgggct tgcgctctgc cagcgctggg
     6001 ctggcctcgg ccatggcctt gccgatttcc tcggcactgc ggccccggct ggccagcttc
     6061 tgcgcggcga taaagtcgca cttgctgagg tcatcaccga agcgcttgac cagcccggcc
     6121 atctcgctgc ggtactcgtc cagcgccgtg cgccggtggc ggctaagctg ccgctcgggc
     6181 agttcgaggc tggccagcct gcgggccttc tcctgctgcc gctgggcctg ctcgatctgc
     6241 tggccagcct gctgcaccag cgccgggcca gcggtggcgg tcttgccctt ggattcacgc
     6301 agcagcaccc acggctgata accggcgcgg gtggtgtgct tgtccttgcg gttggtgaag
     6361 cccgccaagc ggccatagtg gcggctgtcg gcgctggccg ggtcggcgtc gtactcgctg
     6421 gccagcgtcc gggcaatctg cccccgaagt tcaccgcctg cggcgtcggc caccttgacc
     6481 catgcctgat agttcttcgg gctggtttcc actaccaggg caggctcccg gccctcggct
     6541 ttcatgtcat ccaggtcaaa ctcgctgagg tcgtccacca gcaccagacc atgccgctcc
     6601 tgctcggcgg gcctgatata cacgtcattg ccctgggcat tcatccgctt gagccatggc
     6661 gtgttctgga gcacttcggc ggctgaccat tcccggttca tcatctggcc ggtggtggcg
     6721 tccctgacgc cgatatcgaa gcgctcacag cccatggcct tgagctgtcg gcctatggcc
     6781 tgcaaagtcc tgtcgttctt catcgggcca ccaagcgcag ccagatcgag ccgtcctcgg
     6841 ttgtcagtgg cgtcaggtcg agcaagagca acgatgcgat cagcagcacc accgtaggca
     6901 tcatggaagc cagcatcacg gttagccata gcttccagtg ccacccccgc gacgcgctcc
     6961 gggcgctctg cgcggcgctg ctcacctcgg cggctacctc ccgcaactct ttggccagct
     7021 ccacccatgc cgcccctgtc tggcgctggg ctttcagcca ctccgccgcc tgcgcctcgc
     7081 tggcctgctg ggtctggctc atgacctgcc gggcttcgtc ggccagtgtc gccatgctct
     7141 gggccagcgg ttcgatctgc tccgctaact cgttgatgcc tctggatttc ttcactctgt
     7201 cgattgcgtt catggtctat tgcctcccgg tattcctgta agtcgatgat ctgggcgttg
     7261 gcggtgtcga tgttcagggc cacgtctgcc cggtcggtgc ggatgccccg gccttccatc
     7321 tccaccacgt tcggccccag gtgaacaccg ggcaggcgct cgatgccctg cgcctcaagt
     7381 gttctgtggt caatgcgggc gtcgtggcca gcccgctcta atgcccggtt ggcatggtcg
     7441 gcccatgcct cgcgggtctg ctcaagccat gccttgggct tgagcgcttc ggtcttctgt
     7501 gccccgccct tctccggggt cttgccgttg taccgcttga accactgagc ggcgggccgc
     7561 tcgatgccgt cattgatccg ctcggagatc atcaggtggc agtgcgggtt ctcgccgcca
     7621 ccggcatgga tggccagcgt atacggcagg cgctcggcac cggtcaggtg ctgggcgaac
     7681 tcggacgcca gcgccttctg ctggtcgagg gtcagctcga ccggcagggc aaattcgacc
     7741 tccttgaaca gccgcccatt ggcgcgttca tacaggtcgg cagcatccca gtagtcggcg
     7801 ggccgctcga cgaactccgg catgtgcccg gattcggcgt gcaagacttc atccatgtcg
     7861 cgggcatact tgccttcgcg ctggatgtag tcggccttgg ccctggccga ttggccgccc
     7921 gacctgctgc cggttttcgc cgtaaggtga taaatcgcca tgctgcctcg ctgttgcttt
     7981 tgcttttcgg ctccatgcaa tggccctcgg agagcgcacc gcccgaaggg tggccgttag
     8041 gccagtttct cgaagagaaa ccggtaagtg cgccctcccc tacaaagtag ggtcgggatt
     8101 gccgccgctg tgcctccatg atagcctacg agacagcaca ttaacaatgg ggtgtcaaga
     8161 tggttaaggg gagcaacaag gcggcggatc ggctggccaa gctcgaagaa caacgagcgc
     8221 gaatcaatgc cgaaattcag cgggtgcggg caagggaaca gcagcaagag cgcaagaacg
     8281 aaacaaggcg caaggtgctg gtgggggcca tgattttggc caaggtgaac agcagcgagt
     8341 ggccggagga tcggctcatg gcggcaatgg atgcgtacct tgaacgcgac cacgaccgcg
     8401 ccttgttcgg tctgccgcca cgccagaagg atgagccggg ctgaatgatc gaccgagaca
     8461 ggccctgcgg ggctgcacac gcgcccccac ccttcgggta gggggaaagg ccgctaaagc
     8521 ggctaaaagc gctccagcgt atttctgcgg ggtttggtgt ggggtttagc gggctttgcc
     8581 cgcctttccc cctgccgcgc agcggtgggg cggtgtgtag cctagcgcag cgaatagacc
     8641 agctatccgg cctctggccg ggcatattgg gcaagggcag cagcgcccca caagggcgct
     8701 gataaccgcg cctagtggat tattcttaga taatcatgga tggatttttc caacaccccg
     8761 ccagcccccg cccctgctgg gtttgcaggt ttgggggcgt gacagttatt gcaggggttc
     8821 gtgacagtta ttgcaggggg gcgtgacagt tattgcaggg gttcgtgaca gttagtacgg
     8881 gagtgacggg cactggctgg caatgtctag caacggcagg catttcggct gagggtaaaa
     8941 gaactttccg ctaagcgata gactgtatgt aaacacagta ttgcaaggac gcggaacatg
     9001 cctcatgtgg cggccaggac ggccagccgg gatcgggata ctggtcgtta ccagagccac
     9061 cgacccgagc aaacccttct ctatcagatc gttgacgagt attacccggc attcgctgcg
     9121 cttatggcag agcagggaaa ggaattgccg ggctatgtgc aacgggaatt tgaagaattt
     9181 ctccaatgcg ggcggctgga gcatggcttt ctacgggttc gctgcgagtc ttgccacgcc
     9241 gagcacctgg tcgctttcag ctgtaatccg ggcagcgcaa cggaacattc atcagtgtaa
     9301 aaatggaatc aataaagccc tgcgcagcgc gcagggtcag cctgaatacg cgtttaatga
     9361 ccagcacagt cgtgatggca aggtcagaat agcgctgagg tctgcctcgt gaagaaggtg
     9421 ttgctgactc ataccaggcc tgaatcgccc catcatccag ccagaaagtg agggagccac
     9481 ggttgatgag agctttgttg taggtggacc agttggtgat tttgaacttt tgctttgcca
     9541 cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca gcaaaagttc
     9601 gatttattca acaaagccgc cgtcccgtca agtcagcgta atgctctgcc agtgttacaa
     9661 ccaattaacc aattctgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
     9721 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
     9781 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
     9841 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
     9901 atcaccatga gtgacgactg aatccggtga gaatggcaaa agcttatgca tttctttcca
     9961 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
    10021 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca
    10081 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
    10141 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt
    10201 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
    10261 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
    10321 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
    10381 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
    10441 gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc tcatagcacc
    10501 ccttgtatta ctgtttatgt aagcaga
//
