LOCUS       40924_15185        5286 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDS132, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Philippe N, Alcaraz JP, Coursange E, Geiselmann J, Schneider D.
  TITLE     Improvement of pCVD442, a suicide plasmid for gene allele exchange 
            in bacteria
  JOURNAL   Plasmid 51 (3), 246-255 (2004)
  PUBMED    15109831
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Philippe N, Alcaraz JP, Coursange E, Geiselmann J, Schneider D.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-NOV-2003) LAPM, UMR 5163, BP53 CERMO, Grenoble 38041, 
            France
REFERENCE   3  (bases 1 to 5286)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5286)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2004"; volume: "51"; issue: "3"; pages: "246-255"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-NOV-2003) LAPM, UMR 5163, BP53 CERMO, Grenoble 38041, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(4..392)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(1013..1381)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(1414..1523)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(2492..3148)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             complement(3249..4667)
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
     promoter        complement(4668..5113)
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     misc_feature    5135..5208
                     /note="multiple cloning site 
                     (XbaI/SalI/PstI/SalI/PstI/SalI/XbaI/SphI)"
     protein_bind    complement(5239..5255)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ccatgtcagc cgttaagtgt tcctgtgtca ctcaaaattg ctttgagagg ctctaagggc
       61 ttctcagtgc gttacatccc tggcttgttg tccacaaccg ttaaacctta aaagctttaa
      121 aagccttata tattcttttt tttcttataa aacttaaaac cttagaggct atttaagttg
      181 ctgatttata ttaattttat tgttcaaaca tgagagctta gtacgtgaaa catgagagct
      241 tagtacgtta gccatgagag cttagtacgt tagccatgag ggtttagttc gttaaacatg
      301 agagcttagt acgttaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtac
      361 tatcaacagg ttgaactgct gatcttcaga tcctctacgc cggacgcatc gtggccggat
      421 ccagccgacc aggctttcca cgcccgcgtg ccgctccatg tcgttcgcgc ggttctcgga
      481 aacgcgctgc cgcgtttcgt gattgtcacg ctcaagcccg tagtcccgtt cgagcgtcgc
      541 gcagaggtca gcgagggcgc ggtaggcccg atacggctca tggatggtgt ttcgggtcgg
      601 gtgaatcttg ttgatggcga tatggatgtg caggttgtcg gtgtcgtgat gcacggcact
      661 gacgcgctga tgctcggcga agccaagccc agcgcagatg cggtcctcaa tcgcgcgcaa
      721 cgtctccgcg tcgggcttct ctcccgcgcg gaagctaacc agcaggtgat aggtcttgtc
      781 ggcctcggaa cgggtgttgc cgtgctgggt cgccatcacc tcggccatga cagcgggcag
      841 ggtgtttgcc tcgcagttcg tgacgcgcac gtgacccagg cgctcggtct tgccttgctc
      901 gtcggtgatg tacttcacca gctccgcgaa gtcgctcttc ttgatggagc gcatggggac
      961 gtgcttggca atcacgcgca ccccccggcc gttttagcgg ctaaaaaagt catggctctg
     1021 ccctcgggcg gaccacgccc atcatgacct tgccaagctc gtcctgcttc tcttcgatct
     1081 tcgccagcag ggcgaggatc gtggcatcac cgaaccgcgc cgtgcgcggg tcgtcggtga
     1141 gccagagttt cagcaggccg cccaggcggc ccaggtcgcc attgatgcgg gccagctcgc
     1201 ggacgtgctc atagtccacg acgcccgtga ttttgtagcc ctggccgacg gccagcaggt
     1261 aggccgacag gctcatgccg gccgccgccg ccttttcctc aatcgctctt cgttcgtctg
     1321 gaaggcagta caccttgata ggtgggctgc ccttcctggt tggcttggtt tcatcagcca
     1381 tccgcttgcc ctcatctgtt acgccggcgg tagccggcca gcctcgcaga gcaggattcc
     1441 cgttgagcac cgccaggtgc gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg
     1501 ggcctacttc acctatcctg cccggctgac gccgttggat acaccaagga aagtctacac
     1561 gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg
     1621 ctataatgac cccgaagcag ggttatgcag cggaaaagcg ctgcttccct gctgttttgt
     1681 ggaatatcta ccgactggaa acaggcaaat gcaggaaatt actgaactga ggggacaggc
     1741 gagagacgat gccaaagagc tacaccgacg agctggccga gtgggttgaa tcccgcgcgg
     1801 ccaagaagcg ccggcgtgat gaggctgcgg ttgcgttcct ggcggtgagg gcggatgtcg
     1861 aggcggcgtt agcgtccggc tatgcgctcg tcaccatttg ggagcacatg cgggaaacgg
     1921 ggaaggtcaa gttctcctac gagacgttcc gctcgcacgc caggcggcac atcaaggcca
     1981 agcccgccga tgtgcccgca ccgcaggcca aggctgcgga acccgcgccg gcacccaaga
     2041 cgccggagcc acggcggccg aagcaggggg gcaaggctga aaagccggcc cccgctgcgg
     2101 ccccgaccgg cttcaccttc aacccaacac cggacaaaaa ggatcctcta cgccggacgc
     2161 atcgtggccg gcatcaccgg cgccacaagt gcggttgctg gcgcctatat cgccgacatc
     2221 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     2281 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgct gcctcgcgcg
     2341 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     2401 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     2461 gtgtcggggc gcagccatga cccgggaatt acgccccgcc ctgccactca tcgcagtact
     2521 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct
     2581 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
     2641 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
     2701 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
     2761 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
     2821 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
     2881 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg aattccggat
     2941 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
     3001 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
     3061 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
     3121 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgcc ctatgggatt
     3181 cacctttatg ttgataagaa ataaaagaaa atgccaatag gatatcggca ttttcttttg
     3241 cgtttttatt tgttaactgt taattgtcct tgttcaagga tgctgtcttt gacaacagat
     3301 gttttcttgc ctttgatgtt cagcaggaag cttggcgcaa acgttgattg tttgtctgcg
     3361 tagaatcctc tgtttgtcat atagcttgta atcacgacat tgtttccttt cgcttgaggt
     3421 acagcgaagt gtgagtaagt aaaggttaca tcgttaggat caagatccat ttttaacaca
     3481 aggccagttt tgttcagcgg cttgtatggg ccagttaaag aattagaaac ataaccaagc
     3541 atgtaaatat cgttagacgt aatgccgtca atcgtcattt ttgatccgcg ggagtcagtg
     3601 aacaggtacc atttgccgtt cattttaaag acgttcgcgc gttcaatttc atctgttact
     3661 gtgttagatg caatcagcgg tttcatcact tttttcagtg tgtaatcatc gtttagctca
     3721 atcataccga gagcgccgtt tgctaactca gccgtgcgtt ttttatcgct ttgcagaagt
     3781 ttttgacttt cttgacggaa gaatgatgtg cttttgccat agtatgcttt gttaaataaa
     3841 gattcttcgc cttggtagcc atcttcagtt ccagtgtttg cttcaaatac taagtatttg
     3901 tggcctttat cttctacgta gtgaggatct ctcagcgtat ggttgtcgcc tgagctgtag
     3961 ttgccttcat cgatgaactg ctgtacattt tgatacgttt ttccgtcacc gtcaaagatt
     4021 gatttataat cctctacacc gttgatgttc aaagagctgt ctgatgctga tacgttaact
     4081 tgtgcagttg tcagtgtttg tttgccgtaa tgtttaccgg agaaatcagt gtagaataaa
     4141 cggatttttc cgtcagatgt aaatgtggct gaacctgacc attcttgtgt ttggtctttt
     4201 aggatagaat catttgcatc gaatttgtcg ctgtctttaa agacgcggcc agcgtttttc
     4261 cagctgtcaa tagaagtttc gccgactttt tgatagaaca tgtaaatcga tgtgtcatcc
     4321 gcatttttag gatctccggc taatgcaaag acgatgtggt agccgtgata gtttgcgaca
     4381 gtgccgtcag cgttttgtaa tggccagctg tcccaaacgt ccaggccttt tgcagaagag
     4441 atatttttaa ttgtggacga atcgaattca ggaacttgat atttttcatt tttttgctgt
     4501 tcagggattt gcagcatatc atggcgtgta atatgggaaa tgccgtatgt ttccttatat
     4561 ggcttttggt tcgtttcttt cgcaaacgct tgagttgcgc ctcctgccag cagtgcggta
     4621 gtaaaggtta atactgttgc ttgttttgca aactttttga tgttcatcgt tcatgtctcc
     4681 ttttttatgt actgtgttag cggtctgctt cttccagccc tcctgtttga agatggcaag
     4741 ttagttacgc acaataaaaa aagacctaaa atatgtaagg ggtgacgcca aagtatacac
     4801 tttgcccttt acacatttta ggtcttgcct gctttatcag taacaaaccc gcgcgattta
     4861 cttttcgacc tcattctatt agactctcgt ttggattgca actggtctat tttcctcttt
     4921 tgtttgatag aaaatcataa aaggatttgc agactacggg cctaaagaac taaaaaatct
     4981 atctgtttct tttcattctc tgtatttttt atagtttctg ttgcatgggc ataaagttgc
     5041 ctttttaatc acaattcaga aaatatcata atatctcatt tcactaaata atagtgaacg
     5101 gcaggtatat gtgatgggtt aaaaaggatc gatcctctag agtcgacctg caggtcgacg
     5161 gatctgcagg tcgacggatc ccaagcttct tctagaggta ccgcatgcga tatcgagctc
     5221 tcccgggaat tccacaaatt gttatccgct cacaattcca catgtggaat tccacatgtg
     5281 gaattc
//
