LOCUS       40924_14750        6733 bp DNA     circular SYN 13-MAY-2021
DEFINITION  E. Coli/Gram-positive Bacterial Shuttle Vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   LeBlanc DJ, Lee LN, Abu-Al-Jaibat A
  TITLE     Molecular, genetic, and functional analysis of the basic replicon of
            pVA380-1, a plasmid of oral streptococcal origin.
  JOURNAL   Plasmid. 1992 Sep;28(2):130-45.
  PUBMED    1409970
REFERENCE   2  (bases 1 to 6733)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6733)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid."; 
            date: "1992-09"; volume: "28(2)"; pages: "130-45"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(66..83)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(237..825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      5155..5182
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      5314..5357
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(5653..5680)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(5772..5858)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     6332..6348
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6349..6405
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(6418..6434)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6442..6458)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6466..6496)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6511..6532)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
       61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      181 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      241 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      421 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      661 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      841 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      901 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      961 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1021 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1081 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1141 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1201 agaagtggtc cgataaaccc agcgaaccat ttgaggtgat aggtaagatt ataccgaggt
     1261 atgaaaacga gaattggacc tttacagaat tactctatga agcgccatat ttaaaaagct
     1321 accaagacga agaggatgaa gaggatgagg aggcagattg ccttgaatat attgacaata
     1381 ctgataagat aatatatctt ttatatagaa gatcgatttt cgttcgtgaa tacatgttat
     1441 aataactata actaataacg taacgtgact ggcaagagat atttttaaaa caatgaatag
     1501 gtttacactt actttagttt tatggaaatg aaagatcata tcatatataa tctagaataa
     1561 aattaactaa aataattatt atctagataa aaaatttaga agccaatgaa atctataaat
     1621 aaactaaatt aagtttattt aattaacaac tatggatata aaataggtac taatcaaaat
     1681 agtgaggagg atatatttga atacatacga acaaattaat aaagtgaaaa aaatacttcg
     1741 gaaacattta aaaaataacc ttattggtac ttacatgttt ggatcaggag ttgagagtgg
     1801 actaaaacca aatagtgatc ttgacttttt agtcgtcgta tctgaaccat tgacagatca
     1861 aagtaaagaa atacttatac aaaaaattag acctatttca aaaaaaatag gagataaaag
     1921 caacttacga tatattgaat taacaattat tattcagcaa gaaatggtac cgtggaatca
     1981 tcctcccaaa caagaattta tttatggaga atggttacaa gagctttatg aacaaggata
     2041 cattcctcag aaggaattaa attcagattt aaccataatg ctttaccaag caaaacgaaa
     2101 aaataaaaga atatacggaa attatgactt agaggaatta ctacctgata ttccattttc
     2161 tgatgtgaga agagccatta tggattcgtc agaggaatta atagataatt atcaggatga
     2221 tgaaaccaac tctatattaa ctttatgccg tatgatttta actatggaca cgggtaaaat
     2281 cataccaaaa gatattgcgg gaaatgcagt ggctgaatct tctccattag aacataggga
     2341 gagaattttg ttagcagttc gtagttatct tggagagaat attgaatgga ctaatgaaaa
     2401 tgtaaattta actataaact atttaaataa cagattaaaa aaattataaa aaaattgaaa
     2461 aaatggtgga aacacttttt tcaatttttt tgttttatta tttaatattt gggaaatatt
     2521 cattctaatt ggtaatcaga ttttagaaaa caataaaccc ttgcataact ttctcgtcca
     2581 tatcggaaac tactttttta ttcccttttt ttcgaccgag attattttgc gataaaatcc
     2641 gattaagata ctgcctacta actcccaatt cttgagctaa ttctgatact gttttactca
     2701 tcagctacct cacaaaataa ttctttctat cccaattcca aaaggcaaca atttcacgac
     2761 cagtactttc tgcagattct tctgctccgg tctgaacaag attcccttct tcgacatcat
     2821 caagagctaa ctccgccttg attttcttga acaactttcc aaaaccaatc tgcctttttc
     2881 ggtgcaaacc attcgttaaa tcttctgtta tccgttcttt gaccatttca gagaataatg
     2941 tcacatcttc tgtatctaca tcaaaaggct taacaggata tttagctgtt tcgataatag
     3001 ctgacttcaa gtttttacgt ttatgagctt tgacggttcg aatatcaact acaggtgtat
     3061 aatccaattt catcgcctta gcccatagtt tcgtccattc ttcttggcta atgtaatttg
     3121 tattatttcc agtaaaatag ctagatttca caaataacaa tacatgcaaa tgagggtgat
     3181 aattctcatg ctcagttgaa taagttacct cagtggctcg caaatagcca ataacatttt
     3241 tatcgacttt cttgtatttc ataagacgat taaaagctcg ccctatttct gaaattgatt
     3301 tatttaattt ttcaccacta atgttcttaa tcgtcaaggt caaaaagaga aagcgacctt
     3361 ttggatagcg tttcattgct tcatttacca ctaattcagc ttgataagca tacttcattg
     3421 accgtctcca gttacaaatt gggcagagct tatttttgca aaaataagac tgatagagtt
     3481 ttttcgtgcc gtcttgttgt tcgataaact tcaacacttc ggcacattga tagacccgtt
     3541 caaacgaacg atatcctaaa cgctctaaac gaccagcaag ctcaatattt tttaacttgc
     3601 gttctcgcca ctttctatct ttgccattct tagaatagtc cttaaagact tgattttcag
     3661 tacctgtcat gttataattt tcttgcaaaa aaattttttt gagtatataa aaaagttcct
     3721 gcaattctcc taagtttatt attttaaatt ggacacttaa attataaact ttattttatt
     3781 aaattgcaag ggcttttttg ttacctaaaa acccagcaat atcaagggtt tgaggtgctt
     3841 taaaacaaga aaataaaaaa actcattatt tctatatgta tcaagataag aaaaaaccct
     3901 tgctacgcaa ttgggttttt aagggctttt cagccctctt tttcgtttca cgaaaaattc
     3961 acccctaaaa cctcaaattt tgactttcta aatccaaacg tggtataata attttaacgc
     4021 tagaagttct aggaaacgaa tagcacaacg caaaaaaaac agtagctgga actactgtct
     4081 ttcttttttg tcctcaaaaa gaactcaggt taatttttgt cgcgtttgtt tcgaacatac
     4141 cgaatcaacc aatcagcact tacctctgca gcaacgccaa ccacaaaagg tatcaaaaca
     4201 agttctagga aagtcttcac agcgctcacc tccaatctaa gttcgtaact tagcgaaaga
     4261 acgttgcgac ctagctatta taccatgtcc gcttacttta ttccatggtc taattgacaa
     4321 caagtaacca agggcggacg tcctttgtcc gtgtcggctc gaaacgctaa agcctttcgg
     4381 ctcgtcacgt cctagcgtac tttgcccttg cttattgtca attagctttc acggcataaa
     4441 tcgctcaaag gcctagccct ttttcaatca ctcgtttaac tacccttacg attggctgaa
     4501 tagctcttaa ctctgatata aaatttttta aggctttatc tggcataaac tctttcagtt
     4561 ttcctagcat tttgtcattt tgctgtctca aaactgagtt ttcacttcta agcgctctat
     4621 tttcgttcct aagacgctct atgctctctc ccttactttt ttctttagct cgttccaact
     4681 cgcttcaaac cgtttagagc gtctcagagg cactatccgc cttaattgct cattctctag
     4741 ctctttttga tgaacaaggt ttctgcttcg gtatatcagc cctttaaatt cttcaaactg
     4801 ctctttagtc aattccacat attctttacc gaacacgcct tttttagggg taatagccgc
     4861 tagttctttc tcagacatga tttttaggtc agctatttgt gtttttagtt tttctaattt
     4921 cagcccctcg cgctctaaac gctctatttt cgcttctaag ctcttcgaat aattttctaa
     4981 ggtatttata tatccctcag ctttagatag ctctgagagc ctctcagagg tctcagaatc
     5041 gccaggaccc aacgctgccc gactgctttc ctgatgcaaa aacgaggcta gtttaccgta
     5101 tctgtggggg gatggcttgt agatatgacg acaggaagag tttgtagaaa cgcaaaaagg
     5161 ccatccgtca ggatggcctt ctgcttaatt tgatgcctgg cagtttatgg cgggcgtcct
     5221 gcccgccacc ctccgggccg ttgcttcgca acgttcaaat ccgctcccgg cggatttgtc
     5281 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
     5341 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcatggg gagaccccac
     5401 actaccatcg gcgctacggc gtttcacttc tgagttcggc atggggtcag gtgggaccac
     5461 cgcgctactg ccgccaggca aattctgttt tatcagaccg cttctgcgtt ctgatttaat
     5521 ctgtatcagg ctgaaaatct tctctcatcc gccaaaacag ctgctttcct gatgcaaaaa
     5581 cgaggctagt ttaccgtatc tgtgggggga tggcttgtag atatgacgac aggaagagtt
     5641 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttaatttg atgcctggca
     5701 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcgcaac gttcaaatcc
     5761 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
     5821 aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggcagt tccctactct
     5881 cgcatgggga gaccccacac taccatcggc gctacggcgt ttcacttctg agttcggcat
     5941 ggggtcaggt gggaccaccg cgctactgcc gccaggcaaa ttctgtttta tcagaccgct
     6001 tctgcgttct gatttaatct gtatcaggct gaaaatcttc tctcatccgc caaaacagcc
     6061 gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg
     6121 ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg
     6181 aataattccc ggcattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     6241 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     6301 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attcgagctc
     6361 ggtacccggg gatcctctag agtcgacctg caggcatgca agcttggcgt aatcatggtc
     6421 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     6481 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     6541 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     6601 ccaacgcgcg gggagaggcg gtttgcgtat tggagcttgt tgtaactgaa aaaggaaaat
     6661 tattgtgcca ggcagttgaa agtcagcacc ttttaacgag tgctgaaatg acggctaaat
     6721 gggaaacgta ttt
//
