LOCUS       .                       6781 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     rep_origin      complement(237..825)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             1682..2494
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     terminator      5362..5405
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(5701..5728)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(5820..5906)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     6380..6396
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    6397..6453
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(6466..6482)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6490..6506)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6514..6544)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6559..6580)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
       61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      181 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      241 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      421 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      661 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      841 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      901 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      961 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1021 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1081 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1141 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1201 agaagtggtc cgataaaccc agcgaaccat ttgaggtgat aggtaagatt ataccgaggt
     1261 atgaaaacga gaattggacc tttacagaat tactctatga agcgccatat ttaaaaagct
     1321 accaagacga agaggatgaa gaggatgagg aggcagattg ccttgaatat attgacaata
     1381 ctgataagat aatatatctt ttatatagaa gatcgatttt cgttcgtgaa tacatgttat
     1441 aataactata actaataacg taacgtgact ggcaagagat atttttaaaa caatgaatag
     1501 gtttacactt actttagttt tatggaaatg aaagatcata tcatatataa tctagaataa
     1561 aattaactaa aataattatt atctagataa aaaatttaga agccaatgaa atctataaat
     1621 aaactaaatt aagtttattt aattaacaac tatggatata aaataggtac taatcaaaat
     1681 aatgagccat attcaacggg aaacgtcttg ctctaggccg cgattaaatt ccaacatgga
     1741 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
     1801 ctatcgattg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag
     1861 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
     1921 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
     1981 gatccccggg aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
     2041 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
     2101 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt
     2161 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
     2221 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
     2281 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
     2341 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
     2401 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
     2461 attgcagttt catttgatgc tcgatgagtt tttctaaaaa aattgaaaaa atggtggaaa
     2521 cacttttttc aatttttttg ttttattatt taatatttgg gaaatattca ttctaattgg
     2581 taatcagatt ttagaaaaca ataaaccctt gcataacttt ctcgtccata tcggaaacta
     2641 cttttttatt cccttttttt cgaccgagat tattttgcga taaaatccga ttaagatact
     2701 gcctactaac tcccaattct tgagctaatt ctgatactgt tttactcatc agctacctca
     2761 caaaataatt ctttctatcc caattccaaa aggcaacaat ttcacgacca gtactttctg
     2821 cagattcttc tgctccggtc tgaacaagat tcccttcttc gacatcatca agagctaact
     2881 ccgccttgat tttcttgaac aactttccaa aaccaatctg cctttttcgg tgcaaaccat
     2941 tcgttaaatc ttctgttatc cgttctttga ccatttcaga gaataatgtc acatcttctg
     3001 tatctacatc aaaaggctta acaggatatt tagctgtttc gataatagct gacttcaagt
     3061 ttttacgttt atgagctttg acggttcgaa tatcaactac aggtgtataa tccaatttca
     3121 tcgccttagc ccatagtttc gtccattctt cttggctaat gtaatttgta ttatttccag
     3181 taaaatagct agatttcaca aataacaata catgcaaatg agggtgataa ttctcatgct
     3241 cagttgaata agttacctca gtggctcgca aatagccaat aacattttta tcgactttct
     3301 tgtatttcat aagacgatta aaagctcgcc ctatttctga aattgattta tttaattttt
     3361 caccactaat gttcttaatc gtcaaggtca aaaagagaaa gcgacctttt ggatagcgtt
     3421 tcattgcttc atttaccact aattcagctt gataagcata cttcattgac cgtctccagt
     3481 tacaaattgg gcagagctta tttttgcaaa aataagactg atagagtttt ttcgtgccgt
     3541 cttgttgttc gataaacttc aacacttcgg cacattgata gacccgttca aacgaacgat
     3601 atcctaaacg ctctaaacga ccagcaagct caatattttt taacttgcgt tctcgccact
     3661 ttctatcttt gccattctta gaatagtcct taaagacttg attttcagta cctgtcatgt
     3721 tataattttc ttgcaaaaaa atttttttga gtatataaaa aagttcctgc aattctccta
     3781 agtttattat tttaaattgg acacttaaat tataaacttt attttattaa attgcaaggg
     3841 cttttttgtt acctaaaaac ccagcaatat caagggtttg aggtgcttta aaacaagaaa
     3901 ataaaaaaac tcattatttc tatatgtatc aagataagaa aaaacccttg ctacgcaatt
     3961 gggtttttaa gggcttttca gccctctttt tcgtttcacg aaaaattcac ccctaaaacc
     4021 tcaaattttg actttctaaa tccaaacgtg gtataataat tttaacgcta gaagttctag
     4081 gaaacgaata gcacaacgca aaaaaaacag tagctggaac tactgtcttt cttttttgtc
     4141 ctcaaaaaga actcaggtta atttttgtcg cgtttgtttc gaacataccg aatcaaccaa
     4201 tcagcactta cctctgcagc aacgccaacc acaaaaggta tcaaaacaag ttctaggaaa
     4261 gtcttcacag cgctcacctc caatctaagt tcgtaactta gcgaaagaac gttgcgacct
     4321 agctattata ccatgtccgc ttactttatt ccatggtcta attgacaaca agtaaccaag
     4381 ggcggacgtc ctttgtccgt gtcggctcga aacgctaaag cctttcggct cgtcacgtcc
     4441 tagcgtactt tgcccttgct tattgtcaat tagctttcac ggcataaatc gctcaaaggc
     4501 ctagcccttt ttcaatcact cgtttaacta cccttacgat tggctgaata gctcttaact
     4561 ctgatataaa attttttaag gctttatctg gcataaactc tttcagtttt cctagcattt
     4621 tgtcattttg ctgtctcaaa actgagtttt cacttctaag cgctctattt tcgttcctaa
     4681 gacgctctat gctctctccc ttactttttt ctttagctcg ttccaactcg cttcaaaccg
     4741 tttagagcgt ctcagaggca ctatccgcct taattgctca ttctctagct ctttttgatg
     4801 aacaaggttt ctgcttcggt atatcagccc tttaaattct tcaaactgct ctttagtcaa
     4861 ttccacatat tctttaccga acacgccttt tttaggggta atagccgcta gttctttctc
     4921 agacatgatt tttaggtcag ctatttgtgt ttttagtttt tctaatttca gcccctcgcg
     4981 ctctaaacgc tctattttcg cttctaagct cttcgaataa ttttctaagg tatttatata
     5041 tccctcagct ttagatagct ctgagagcct ctcagaggtc tcagaatcgc caggacccaa
     5101 cgctgcccga ctgctttcct gatgcaaaaa cgaggctagt ttaccgtatc tgtgggggga
     5161 tggcttgtag atatgacgac aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg
     5221 atggccttct gcttaatttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct
     5281 ccgggccgtt gcttcgcaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga
     5341 gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt
     5401 tttatttgat gcctggcagt tccctactct cgcatgggga gaccccacac taccatcggc
     5461 gctacggcgt ttcacttctg agttcggcat ggggtcaggt gggaccaccg cgctactgcc
     5521 gccaggcaaa ttctgtttta tcagaccgct tctgcgttct gatttaatct gtatcaggct
     5581 gaaaatcttc tctcatccgc caaaacagct gctttcctga tgcaaaaacg aggctagttt
     5641 accgtatctg tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca
     5701 aaaaggccat ccgtcaggat ggccttctgc ttaatttgat gcctggcagt ttatggcggg
     5761 cgtcctgccc gccaccctcc gggccgttgc ttcgcaacgt tcaaatccgc tcccggcgga
     5821 tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct
     5881 ttcgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg catggggaga
     5941 ccccacacta ccatcggcgc tacggcgttt cacttctgag ttcggcatgg ggtcaggtgg
     6001 gaccaccgcg ctactgccgc caggcaaatt ctgttttatc agaccgcttc tgcgttctga
     6061 tttaatctgt atcaggctga aaatcttctc tcatccgcca aaacagccga gatgcgccgc
     6121 gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca
     6181 ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa taattcccgg
     6241 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     6301 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     6361 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcgagctcgg tacccgggga
     6421 tcctctagag tcgacctgca ggcatgcaag cttggcgtaa tcatggtcat agctgtttcc
     6481 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     6541 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6601 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6661 gagaggcggt ttgcgtattg gagcttgttg taactgaaaa aggaaaatta ttgtgccagg
     6721 cagttgaaag tcagcacctt ttaacgagtg ctgaaatgac ggctaaatgg gaaacgtatt
     6781 t
//
