LOCUS       V013018                 6448 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013018
VERSION     V013018
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1078..1182
                     /label="AmpR promoter"
     CDS             1183..2040
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2214..2802
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5506..6153)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
ORIGIN
        1 atatcccact ttatccaatt ttcgtttgtt gaactaatgg gtgctttagt tgaagaataa
       61 aagaccacat taaaaaatgt ggtcttttgt gttttttaaa ggatttgagc gtagcgaaaa
      121 atccttttct ttcttatctt gataataagg gtaactattg ccgatgataa gctgtcaaac
      181 atgagaattc gataagcttc taggatcctg agcgccggtc gctaccatta ccagttggtc
      241 tggtgtcaaa aataataata accgggcagg ccatgtctgc ccgtatttcg cgtaaggaaa
      301 tccattatgt actatttaat tctgcgtgac atcccatcga tcagaccagt ttttaatttg
      361 tgtgtttcca tgtgtccagt ttggaatact cttaacctca ttggaaatcg cggcataatc
      421 actggtggta tgattgatga ccgcgtcaac aatgaccttt atgccatatt cttcagcggc
      481 tgcacacatt tctttaaatt cttgttcagt acctaagtaa cggttgccaa tttgatacga
      541 tgtcggctga tacagccagt accagttcga catgctttta tctccttgat tcccttcctt
      601 tacttggtta atcggagatg tctgaatggc tgtatatcct gcatcatgaa tatccttcat
      661 attgtgtttt aacgtattga acgaccaatt ccatgcatga agaatggttc cgcttttgat
      721 cgacggtgct gtaagctcat tcgatttgtt cgccgtttca gcactcgcag ccgccggtcc
      781 tgccagaacc aaatgaaaca gcaataaaaa tccagcgaat aacggcagta aagaggtttt
      841 gaatcgtttt gcaaacattc ttgacactcc ttatttgatt ttttgaagac ttacttcgga
      901 gtcaaaaatc cctcttactt cattcttccg cttcctcctt tcaaaccgat gtgaagactg
      961 gagaattttg ttaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     1021 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     1081 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1141 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1201 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1261 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1321 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1381 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     1441 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1501 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1561 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1621 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     1681 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
     1741 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     1801 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     1861 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     1921 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     1981 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2041 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2101 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2161 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2221 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2281 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2341 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     2401 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2461 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2521 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2581 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2641 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2701 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2761 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2821 cgacctcgag ctggatactt cccgtccgcc agggggacat gccggcgatg ctgaaggtcg
     2881 cgcgcattcc cgatgaagag gccggttacc gcctgtttga ggatatagta atctttctaa
     2941 atagctttgg attggaggag tatggccact aatactaagt tcagctaata aaaaaatttg
     3001 ctaaagaact ccagctggat ttcactgatg agaatatcgt cggagataaa tataataatt
     3061 ccacggacta tagactatac tagtatactc cgtctactgt acgatacact tccgctcagg
     3121 tccttgtcct ttaacgagga ttgttacgta cgctaatggc gtcaaaacaa agactctaga
     3181 cctaggcctt aagatctgat catatgcatc cgcgggcccg ggttaacgcg taatccatgg
     3241 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     3301 ctccggtgcc ctgaatgaac tgcagaaaga gctggtagtt ggcgcactgt tcgaagaact
     3361 gccgatgtcc agtaagattc ttactatgct ggttgaaccg gatgctggta aagctacttg
     3421 ggttgctgct tctacttatg gtaccgatac aactactggt gaggaagtta aaggagctct
     3481 taaagaaatc cacttcagta ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg
     3541 cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag
     3601 gcggcataaa tcgccgtgac gatcagcggt ccaatgatcg aagttaggct ggtaagagcc
     3661 gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc
     3721 tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc
     3781 cagcctcgcg tcgcgactaa gaaaatgccg tcaaatccgc tcgccatgac ttcactaacg
     3841 atgcctttga aaatcttcaa gttcttttct actaattcaa ggcgtgtctc accaggtttt
     3901 tggtttgctc cggcgcaaat gcagacaata tcagcatcct tgcagggtat gtttctcttt
     3961 gatgtctttt tgtttgtgaa gtatttcaca tttatattgt gcaacacttc acaaactttt
     4021 gcaagagaaa agttttgtct gatttatgaa caaaaaagaa accatcattg atggtttctt
     4081 tcggtaagtc ccgtctagcc ttgccctcaa tggggaagag aaccgcttaa gcccgagtca
     4141 ttatataaac catttagcac gtaatcaaag ccaggctgat tctgaccggg cacttgggcg
     4201 ctgccattat taaaaatcac ttttgcgttg gttgtatccg tgtccgcagg cagcgtcagc
     4261 gtgtaaattc cgtctgcatt tttagtcatt ggttttccag gccaagatcc ggtcaattca
     4321 attactcggc tcccatcatg tttatagata taagcattta cctggctcca atgattcgga
     4381 ttttgatagc cgatggtttt ggccgacgct ggatctcttt taacaaaact gtatttctcg
     4441 gtcctcgtta caccatcact gttcgttcct tttaacatga tggtgtatgt tttgccaaat
     4501 ggatctcctt ttccgattgt gaattgatct ccatccttaa acgccgtctc tggtccatta
     4561 ttgatttgat aaacggcttt tgttgtattc gcatctgcac gcaaggtaat cgtcagttga
     4621 tcattgaaag aatgtgttac acctgttttg taattctcaa ggaaaacatg aggcgctttt
     4681 gcaatatcat caggataaag cacagctaca gacctggcat tgatcgtgcc tgtcagttta
     4741 ccatcgttca cttgaaatga acccgctcca gctttattgt catacctgcc atcaggcaat
     4801 tttgttgccg tattgataga gacagaggat gaacctgcat ttgccagcac aacgccatgt
     4861 gagccgcgct gattcataaa tatctggttg tttccattcg ggttcgagag ttcctcaggc
     4921 tgtccagcca tcacattgtg aaatctattg accgcagtga tagcctgatc ttcaaataaa
     4981 gcactcccgc gatcgcctat ttggcttttc cccgggaacc tcacaccatt tccgcctccc
     5041 tcaggtctgg aaaagaaaag aggcgtactg cctgaacgag aagctatcac cgcccagcct
     5101 aaacggatat catcatcgct catccatgtc gacgctctcc cttatgcgac tcctgcatta
     5161 ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat
     5221 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
     5281 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
     5341 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
     5401 agaggatctg gagctgtaat ataaaaacct tcttcaacta acggggcagg ttagtgacat
     5461 tagaaaaccg actgtaaaaa gtacagtcgg cattatctca tattataaaa gccagtcatt
     5521 aggcctatct gacaattcct gaatagagtt cataaacaat cctgcatgat aaccatcaca
     5581 aacagaatga tgtacctgta aagatagcgg taaatatatt gaattacctt tattaatgaa
     5641 ttttcctgct gtaataatgg gtagaaggta attactatta ttattgatat ttaagttaaa
     5701 cccagtaaat gaagtccatg gaataataga aagagaaaaa gcattttcag gtataggtgt
     5761 tttgggaaac aatttccccg aaccattata tttctctaca tcagaaaggt ataaatcata
     5821 aaactctttg aagtcattct ttacaggagt ccaaatacca gagaatgttt tagatacacc
     5881 atcaaaaatt gtataaagtg gctctaactt atcccaataa cctaactctc cgtcgctatt
     5941 gtaaccagtt ctaaaagctg tatttgagtt tatcaccctt gtcactaaga aaataaatgc
     6001 agggtaaaat ttatatcctt cttgttttat gtttcggtat aaaacactaa tatcaatttc
     6061 tgtggttata ctaaaagtcg tttgttggtt caaataatga ttaaatatct cttttctctt
     6121 ccaattgtct aaatcaattt tattaaagtt catttgatat gcctcctaaa tttttatcta
     6181 aagtgaattt aggaggctta cttgtctgct ttcttcatta gaatcaatcc ttttttaaaa
     6241 gtcaatatta ctgtaacata aatatatatt ttaaaaatat cccactttat ccaattttcg
     6301 tttgttgaac taatgggtgc tttagttgaa gaataaaaga ccacattaaa aaatgtggtc
     6361 ttttgtgttt ttttaaagga tttgagcgta gcgaaaaatc cttttctttc ttatcttgat
     6421 aataagggta actattgccg atgataag
//
