LOCUS       .                       9292 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    270..286
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     CDS             310..1338
                     /label="Cre"
                     /note="site-specific recombinase"
     CDS             1590..2669
                     /label="lacI"
                     /note="lac repressor"
     misc_feature    2843..2983
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3169..3757)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3931..4788)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(4789..4893)
                     /label="AmpR promoter"
     CDS             5412..6413
                     /label="repB"
                     /note="RepB replication protein"
ORIGIN
        1 gaattctaca cagcccagtc cagactattc ggcactgaaa ttatgggtga agtggtcaag
       61 acctcactag gcaccttaaa aatagcgcac cctgaagaag atttatttga ggtagccctt
      121 gcctacctag cttccaagaa agatatccta acagcacaag agcggaaaga tgttttgttc
      181 tacatccaga acaacctctg ctaaaattcc tgaaaaattt tgcaaaaagt tgttgacttt
      241 atctacaagg tgtggcataa tgtgtggaat tgtgagcgga taacaattaa gcttaaggag
      301 gaagcaggta tgtccaattt actgaccgta caccaaaatt tgcctgcatt accggtcgat
      361 gcaacgagtg atgaggttcg caagaacctg atggacatgt tcagggatcg ccaggcgttt
      421 tctgagcata cctggaaaat gcttctgtcc gtttgccggt cgtgggcggc atggtgcaag
      481 ttgaataacc ggaaatggtt tcccgcagaa cctgaagatg ttcgcgatta tcttctatat
      541 cttcaggcgc gcggtctggc agtaaaaact atccagcaac atttgggcca gctaaacatg
      601 cttcatcgtc ggtccgggct gccacgacca agtgacagca atgctgtttc actggttatg
      661 cggcggatcc gaaaagaaaa cgttgatgcc ggtgaacgtg caaaacaggc tctagcgttc
      721 gaacgcactg atttcgacca ggttcgttca ctcatggaaa atagcgatcg ctgccaggat
      781 atacgtaatc tggcatttct ggggattgct tataacaccc tgttacgtat agccgaaatt
      841 gccaggatca gggttaaaga tatctcacgt actgacggtg ggagaatgtt aatccatatt
      901 ggcagaacga aaacgctggt tagcaccgca ggtgtagaga aggcacttag cctgggggta
      961 actaaactgg tcgagcgatg gatttccgtc tctggtgtag ctgatgatcc gaataactac
     1021 ctgttttgcc gggtcagaaa aaatggtgtt gccgcgccat ctgccaccag ccagctatca
     1081 actcgcgccc tggaagggat ttttgaagca actcatcgat tgatttacgg cgctaaggat
     1141 gactctggtc agagatacct ggcctggtct ggacacagtg cccgtgtcgg agccgcgcga
     1201 gatatggccc gcgctggagt ttcaataccg gagatcatgc aagctggtgg ctggaccaat
     1261 gtaaatattg tcatgaacta tatccgtaac ctggatagtg aaacaggggc aatggtgcgc
     1321 ctgctggaag atggcgatta ggcatgcaag ctaattcggt ggaaacgagg tcatcatttc
     1381 cttccgaaaa aacggttgca tttaaatctt acatatgtaa tactttcaaa gactacattt
     1441 gtaagatttg atgtttgagt cggctgaaag atcgtacgta ccaattattg tttcgtgatt
     1501 gttcaagcca taacactgta gggatagtgg aaagagtgct tcatctggtt acgatcaatc
     1561 aaatattcaa acggagggag acgattttga tgaaaccagt aacgttatac gatgtcgcag
     1621 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt
     1681 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc
     1741 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc
     1801 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg
     1861 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaaacggcgg
     1921 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc
     1981 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct
     2041 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg
     2101 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt
     2161 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
     2221 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
     2281 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
     2341 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
     2401 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac
     2461 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
     2521 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
     2581 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     2641 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttaggat
     2701 cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     2761 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     2821 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
     2881 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     2941 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
     3001 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     3061 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     3121 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     3181 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     3241 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3301 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3361 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3421 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3481 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3541 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3601 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3661 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3721 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3781 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3841 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3901 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     3961 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     4021 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     4081 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     4141 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     4201 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc
     4261 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     4321 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     4381 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     4441 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     4501 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc
     4561 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     4621 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4681 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     4741 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4801 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4861 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     4921 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     4981 ctttcgtctt caagaattcc ttaaggaacg tacagacggc ttaaaagcct ttaaaaacgt
     5041 ttttaagggg tttgtagaca aggtaaagga taaaacagca caattccaag aaaaacacga
     5101 tttagaacct aaaaagaacg aatttgaact aactcataac cgagaggtaa aaaaagaacg
     5161 aagtcgagat cagggaatga gtttataaaa taaaaaaagc acctgaaaag gtgtcttttt
     5221 ttgatggttt tgaacttgtt ctttcttatc ttgatacata tagaaataac gtcattttta
     5281 ttttagttgc tgaaaggtgc gttgaagtgt tggtatgtat gtgttttaaa gtattgaaaa
     5341 cccttaaaat tggttgcaca gaaaaacccc atctgttaaa gttataagtg actaaacaaa
     5401 taactaaata gatggggttt tcttttaata ttatgtgtcc taatagtagc atttattcag
     5461 atgaaaaatc aagggtttta gtggacaaga caaaaagtgg aaaagtgaga ccatggagag
     5521 aaaagaaaat cgctaatgtt gattactttg aacttctgca tattcttgaa tttaaaaagg
     5581 ctgaaagagt aaaagattgt gctgaaatat tagagtataa acaaaatcgt gaaacaggcg
     5641 aaagaaagtt gtatcgagtg tggttttgta aatccaggct ttgtccaatg tgcaactgga
     5701 ggagagcaat gaaacatggc attcagtcac aaaaggttgt tgctgaagtt attaaacaaa
     5761 agccaacagt tcgttggttg tttctcacat taacagttaa aaatgtttat gatggcgaag
     5821 aattaaataa gagtttgtca gatatggctc aaggatttcg ccgaatgatg caatataaaa
     5881 aaattaataa aaatcttgtt ggttttatgc gtgcaacgga agtgacaata aataataaag
     5941 ataattctta taatcagcac atgcatgtat tggtatgtgt ggaaccaact tattttaaga
     6001 atacagaaaa ctacgtgaat caaaaacaat ggattcaatt ttggaaaaag gcaatgaaat
     6061 tagactatga tccaaatgta aaagttcaaa tgattcgacc gaaaaataaa tataaatcgg
     6121 atatacaatc ggcaattgac gaaactgcaa aatatcctgt aaaggatacg gattttatga
     6181 ccgatgatga agaaaagaat ttgaaacgtt tgtctgattt ggaggaaggt ttacaccgta
     6241 aaaggttaat ctcctatggt ggtttgttaa aagaaataca taaaaaatta aaccttgatg
     6301 acacagaaga aggcgatttg attcatacag atgatgacga aaaagccgat gaagatggat
     6361 tttctattat tgcaatgtgg aattgggaac ggaaaaatta ttttattaaa gagtagttca
     6421 acaaacgggc cagtttgttg aagattagat gctataattg ttattaaaag gattgaagga
     6481 tgcttaggaa gacgagttat taatagctga ataagaacgg tgctctccaa atattcttat
     6541 ttagaaaagc aaatctaaaa ttatctgaaa agggaatgag aatagtgaat ggaccaataa
     6601 taatgactag agaagaaaga atgaagattg ttcatgaaat taaggaacga atattggata
     6661 aatatgggga tgatgttaag gctattggtg tttatggctc tcttggtcgt cagactgatg
     6721 ggccctattc ggatattgag atgatgtgtg tcatgtcaac agaggaagca gagttcagcc
     6781 atgaatggac aaccggtgag tggaaggtgg aagtgaattt tgatagcgaa gagattctac
     6841 tagattatgc atctcaggtg gaatcagatt ggccgcttac acatggtcaa tttttctcta
     6901 ttttgccgat ttatgattca ggtggatact tagagaaagt gtatcaaact gctaaatcgg
     6961 tagaagccca aacgttccac gatgcgattt gtgcccttat cgtagaagag ctgtttgaat
     7021 atgcaggcaa atggcgtaat attcgtgtgc aaggaccgac aacatttcta ccatccttga
     7081 ctgtacaggt agcaatggca ggtgccatgt tgattggtct gcatcatcgc atctgttata
     7141 cgacgagcgc ttcggtctta actgaagcag ttaagcaatc agatcttcct tcaggttatg
     7201 accatctgtg ccagttcgta atgtctggtc aactttccga ctctgagaaa cttctggaat
     7261 cgctagagaa tttctggaat gggattcagg agtggacaga acgacacgga tatatagtgg
     7321 atgtgtcaaa acgcatacca ttttgaacga tgacctctaa taattgttaa tcatgttggt
     7381 tacgtattta ttaacttctc ctagtattag taattatcat ggctgtcatg gcgcattaac
     7441 ggaataaagg gtgtgcttaa atcgggccat tttgcgtaat aagaaaaagg attaattatg
     7501 agcgaattga attaataata aggtaataga tttacattag aaaatgaaag gggattttat
     7561 gcgtgagaat gttacagtct atcccggcat tgccagtcgg ggatattaaa aagagtatag
     7621 gtttttattg cgataaacta ggtttcactt tggttcacca tgaagatgga ttcgcagttc
     7681 taatgtgtaa tgaggttcgg attcatctat gggaggcaag tgatgaaggc tggcgctctc
     7741 gtagtaatga ttcaccggtt tgtacaggtg cggagtcgtt tattgctggt actgctagtt
     7801 gccgcattga agtagaggga attgatgaat tatatcaaca tattaagcct ttgggcattt
     7861 tgcaccccaa tacatcatta aaagatcagt ggtgggatga acgagacttt gcagtaattg
     7921 atcccgacaa caatttgatt agcttttttc aacaaataaa aagctaaaat ctattattaa
     7981 tctgttcagc aatcgggcgc gattgctgaa taaaagatac gagagacctc tcttgtatct
     8041 tttttatttt gagtggtttt gtccgttaca ctagaaaacc gaaagacaat aaaaatttta
     8101 ttcttgctga gtctggcttt cggtaagcta gacaaaacgg acaaaataaa aattggcaag
     8161 ggtttaaagg tggagatttt ttgagtgatc ttctcaaaaa atactacctg tcccttgctg
     8221 atttttaaac gagcacgaga gcaaaacccc cctttgctga ggtggcgaac gatgagccaa
     8281 taatacgtac agacataata gtgccagcca gcagcagggc gtttttccca ccgatgcgat
     8341 taatgatcag tggcgcaaag aacataatcg aggcgttaag taattcgccc attgtcgtta
     8401 cgtagccaaa tacccgcgta ccctgttcac cggtagcaaa gaacgaagta aagaaattag
     8461 caaactgttg gtcaaaaaca tcgtaggtgc aggaaacgcc aataacatac agtgacaaaa
     8521 accacagttt tggctgtctg aacagttcca gtgccagctt aaggctaaat gccgaatggt
     8581 tggcacctac cgcattggca accgtggcag aagagggcgc atccgttttg gcgaaaaaga
     8641 gtaaaacggc gaggatgagt gcacagccag agcccagcca gaaaacaaac tgattattga
     8701 tggtgaacat gatgccgaca atcgaggcac acagcgccca gccaacacag ccaaacatcc
     8761 gcgcgcgacc aaattcgaaa ttactgcgac ggctgacttt ctcaataaat gcctctactg
     8821 ctggcgcacc ggcgttaaaa caaaagccta gataaatacc accaacaatc gatcctacta
     8881 aaatgttgta ttgtaacagt ggcccgaaga taaaaataaa gaacggcgca aacatcacta
     8941 acatgccggt aataatccac agcaggtatt tgcgcagccc gagtttgtca gaaagcagac
     9001 caaacagcgg ttggaataat agcgagaaca gagaaatagc ggcaaaaata atacccgtat
     9061 cacttttgct gatatggttg atgtcatgta gccaaatcgg gaaaaacggg aagtaggctc
     9121 ccatgataaa aaagtaaaag aaaaagaata aaccgaacat ccaaaagttt gtgtttttta
     9181 aatagtacat aatggatttc cttacgcgaa atacgggcag acatggcctg cccggttatt
     9241 attatttttg acaccagacc aactggtaat ggtagcgacc ggcgctcagc tg
//
