LOCUS pDC316-RIPK3(human) 5465 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V020452 VERSION V020452 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers misc_signal 190..340 /note="packaging signal for adenovirus serotype 5" /label="Ad5 Ψ" promoter 470..993 /label="mCMV promoter" CDS 1006..2559 /label="RIPK3(NM_006871)" /note="RIPK3(NM_006871)" /gene="RIPK3" polyA_signal 2588..2722 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" protein_bind complement(2742..2775) /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (Shaw et al., 2021)." /label="loxP" primer_bind complement(2797..2813) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 2821..2837 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(2845..2875) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 2890..2911 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(3199..3787) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(3958..4818) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(4819..4923) /gene="bla" /label="AmpR promoter" ORIGIN 1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt 61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg 361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc 421 gggtcaaagt tggcgtttta ttattatagt cagctctaga gatatactga gtcattaggg 481 actttccaat gggttttgcc cagtacataa ggtcaatagg ggtgaatcaa caggaaagtc 541 ccattggagc caagtacact gagtcaatag ggactttcca ttgggttttg cccagtacaa 601 aaggtcaata gggggtgagt caatgggttt ttcccattat tggcacgtac ataaggtcaa 661 taggggtgag tcattgggtt tttccagcca atttaattaa aacgccatgt actttcccac 721 cattgacgtc aatgggctat tgaaactaat gcaacgtgac ctttaaacgg tactttccca 781 tagctgatta atgggaaagt accgttctcg agccaataca cgtcaatggg aagtgaaagg 841 gcagccaaaa cgtaacaccg ccccggtttt cccctggaaa ttccatattg gcacgcattc 901 tattggctga gctgcgttct acgtgggtat aagaggcgcg accagcgtcg gtaccgtcgc 961 agtcttcggt ctgaccaccg tagaacgcag atcgaattcg ccaccatgtc gtgcgtcaag 1021 ttatggccca gcggtgcccc cgcccccttg gtgtccatcg aggaactgga gaaccaggag 1081 ctcgtcggca aaggcgggtt cggcacagtg ttccgggcgc aacataggaa gtggggctac 1141 gatgtggcgg tcaagatcgt aaactcgaag gcgatatcca gggaggtcaa ggccatggca 1201 agtctggata acgaattcgt gctgcgccta gaaggggtta tcgagaaggt gaactgggac 1261 caagatccca agccggctct ggtgactaaa ttcatggaga acggctcctt gtcggggctg 1321 ctgcagtccc agtgccctcg gccctggccg ctcctttgcc gcctgctgaa agaagtggtg 1381 cttgggatgt tttacctgca cgaccagaac ccggtgctcc tgcaccggga cctcaagcca 1441 tccaacgtcc tgctggaccc agagctgcac gtcaagctgg cagattttgg cctgtccaca 1501 tttcagggag gctcacagtc agggacaggg tccggggagc cagggggcac cctgggctac 1561 ttggccccag aactgtttgt taacgtaaac cggaaggcct ccacagccag tgacgtctac 1621 agcttcggga tcctaatgtg ggcagtgctt gctggaagag aagttgagtt gccaaccgaa 1681 ccatcactcg tgtacgaagc agtgtgcaac aggcagaacc ggccttcatt ggctgagctg 1741 ccccaagccg ggcctgagac tcccggctta gaaggactga aggagctaat gcagctctgc 1801 tggagcagtg agcccaagga cagaccctcc ttccaggaat gcctaccaaa aactgatgaa 1861 gtcttccaga tggtggagaa caatatgaat gctgctgtct ccacggtaaa ggatttcctg 1921 tctcagctca ggagcagcaa taggagattt tctatcccag agtcaggcca aggagggaca 1981 gaaatggatg gctttaggag aaccatagaa aaccagcact ctcgtaatga tgtcatggtt 2041 tctgagtggc taaacaaact gaatctagag gagcctccca gctctgttcc taaaaaatgc 2101 ccgagcctta ccaagaggag cagggcacaa gaggagcagg ttccacaagc ctggacagca 2161 ggcacatctt cagattcgat ggcccaacct ccccagactc cagagacctc aactttcaga 2221 aaccagatgc ccagccctac ctcaactgga acaccaagtc ctggaccccg agggaatcag 2281 ggggctgaga gacaaggcat gaactggtcc tgcaggaccc cggagccaaa tccagtaaca 2341 gggcgaccgc tcgttaacat atacaactgc tctggggtgc aagttggaga caacaactac 2401 ttgactatgc aacagacaac tgccttgccc acatggggct tggcaccttc gggcaagggg 2461 aggggcttgc agcacccccc accagtaggt tcgcaagaag gccctaaaga tcctgaagcc 2521 tggagcaggc cacagggttg gtataatcat agcgggaaat aaaagcttga gctcgtcgac 2581 ttcgagcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 2641 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 2701 tgtatcttat catgtctgga tcgtctagca tcgaagatcc aataacttcg tatagcatac 2761 attatacgaa gttataagta gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 2821 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 2881 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 2941 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 3001 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 3061 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 3121 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 3181 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 3241 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 3301 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 3361 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc 3421 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 3481 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 3541 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 3601 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 3661 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 3721 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 3781 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 3841 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 3901 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 3961 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 4021 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 4081 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 4141 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 4201 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 4261 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 4321 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 4381 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 4441 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 4501 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 4561 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat 4621 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 4681 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 4741 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 4801 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 4861 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 4921 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt 4981 aacctataaa aataggcgta tcactctagg caaaatagca ccctcccgct ccagaacaac 5041 atacagcgct tcacagcggc agcctaacag tcagccttac cagtaaaaaa gaaaacctat 5101 taaaaaaaca ccactcgaca cggcaccagc tcaatcagtc acagtgtaaa aaagggccaa 5161 gtgcagagcg agtatatata ggactaaaaa atgacgtaac ggttaaagtc cacaaaaaac 5221 acccagaaaa ccgcacgcga acctacgccc agaaacgaaa gccaaaaaac ccacaacttc 5281 ctcaaatcgt cacttccgtt ttcccacgtt acgtaacttc ccattttaag aaaactacaa 5341 ttcccaacac atacaagtta ctccgcccta aaacctacgt cacccgcccc gttcccacgc 5401 cccgcgccac gtcacaaact ccaccccctc attatcatat tggcttcaat ccaaaataag 5461 gtata //