LOCUS       40924_13940        7804 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pCX43luc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7804)
  AUTHORS   van Tuyn J, Pijnappels DA, de Vries AA, de Vries I, van der 
            Velde-van Dijke I, Knaan-Shanzer S, van der Laarse A, Schalij MJ, 
            Atsma DE.
  TITLE     Fibroblasts from human postmyocardial infarction scars acquire 
            properties of cardiomyocytes after transduction with a recombinant 
            myocardin gene
  JOURNAL   FASEB J. 21 (12), 3369-3379 (2007)
  PUBMED    17579192
REFERENCE   2  (bases 1 to 7804)
  AUTHORS   van Tuyn J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 
            RC, Netherlands
REFERENCE   3  (bases 1 to 7804)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7804)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J."; 
            date: "2007"; volume: "21"; issue: "12"; pages: "3369-3379"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 RC, 
            Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    7..55
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    69..160
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     regulatory      180..3189
                     /label=Homo sapiens Cx43 (connexin 43)
                     /note="Homo sapiens Cx43 (connexin 43)"
                     /regulatory_class="promoter"
     CDS             3241..4890
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(4934..5055)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(5474..6062)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6236..7093)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7094..7198)
                     /label=AmpR promoter
     rep_origin      7225..7680
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg
       61 atagtactaa catacgctct ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag
      121 gctgtcccca gtgcaagtgc aggtgccaga acatttctct atcgataggt accgcggccg
      181 catggagaaa acagaatcaa aggcctggca ggtcatttgg cctaaagaag ccctgcagaa
      241 gacagaaact gtaaataact gtttctagtt ctccaaagag cagtgttaat caggctgcat
      301 ccatttcttc tacatagaca aggtctggca atcagagaag acacagttct aggaagtcag
      361 tgtttatatt aaagaaatgg cattgacatt tctgagaata tgagaaaaac acatttaaaa
      421 aaaaaaaact attccacaat ttttgcaaaa cattccaatg gagacaagct tctaaggtca
      481 gataagtgag atacttttga acaattttgc tattgataaa aatataactc accaacactg
      541 ctagagtatg agttataata gaagttgcta cctgtcattt agagaccctg ttagttgtcc
      601 atgagagaaa actgtggggc cttttgcaaa acctagtttg tcttgcacat gattactcat
      661 agcattgcat ttcagtgcca ttatcccaaa agaagagaag cttcttcaga atgtcttatc
      721 aatgatattt tcatgaaatc atgttgagat cttgtatcaa tgatcatgga aaaatgattt
      781 tagaaagaaa agacatagta cgttcatgta ttaagagaag taactaatta ctggctcatg
      841 ctatgagtaa ttcctttggc tcagaaaata gttcaagtga tatccatgaa taatttaact
      901 tgttataagg aagttcttta gctctatttc caattggtca cataggtaat agattaaaaa
      961 gaaaagaata ctttacaaga atatttaaac atcaacattc atctatagac ctaacaaaac
     1021 ataacagaaa taaacacaat tttttttgct ttaaaaatcc tattataaaa cagtcaatga
     1081 caacttttct ttcatgtaac ttattctgaa ttagcatcat ggtaatgttt tttataattg
     1141 tagttgataa ataattttac aaaaataaat tgttatgtaa attaaatgaa gcatactttt
     1201 ttgaaaagaa gacatgtatt ttcttacgat aaaaagaata aatcattttc atgaatatgt
     1261 tctaagtcca atgaaataat ttttagttaa gacaaaacaa tagatttatt taaagaacaa
     1321 tataaataca agaccaaaac aacatttata ggttgctgaa cctgaagact cgaaagtcag
     1381 gttacaattt ctacttccag aagattgttt ttcccctttc ttaatttaaa gaaaaatgta
     1441 ctcttggaaa taggatagcc aacaagtaaa agcaaaattg ttccttaaaa ttgcaacatt
     1501 tgggcccagc gcggcggctc acgcctgtga tcccagcact ttgggaggcg gaggcctgta
     1561 atcccagcca cccaggaggc tgaggcagga gaatcacttg aagccggaag gcggaggttg
     1621 cggtgagcag agatggcgcc actgcactct agtttgggca acaagagcga aactccgtct
     1681 cagaaaagaa aaaaaaaaac ctgcaacatt tcaaaagatg ttatattagt catttcagtg
     1741 aaagtttaat tagggaacgg tgtctaccag aatatatttg gcatccttct tttttctggt
     1801 atttgcctga gtgagaaaag tgttaggaat aggaaggcca gatgagtttg ctttccctcc
     1861 agagaatact ggccaagaaa tttcctgtcc aacctcagga gcatcactga agcctgttta
     1921 tgaggaattg tcctaccaca acactgtgtt atggcatttg ggaagccctc agaggagctg
     1981 cctgctctaa gattgaggcc acatgactca ggccattata aaccctaatg cagagccctg
     2041 aagagggaga gcatttggaa ggggataggg aagcacataa agatgatatg ctcagcttta
     2101 ctgtcgttgg gcagaaaact ctctagtggg cttgagaggg ggaactgatt ttaaggaggg
     2161 ctgaatagtt tgggaaccta cttaatgcaa ccatagtctg tatttttaaa atcctcacat
     2221 ttcactacag atttgggttt tagtgtcttg gcataaatag ggccttaata taaattctta
     2281 gttttctgga gacatggtag aggtcagata tcaagatttc ataggcaaac aaaacatgct
     2341 atatatgtaa aaaatgtata tacacacaca gccttttgct aaactgaaag aaagtaaaaa
     2401 ttcaaagcag tcaaggggtt gaaagatgga ttgggtatgg acttttcttc atctatgtca
     2461 atttttgaaa tctctctccc atctccacca ttccctttgt taattgtcct tgcttacatt
     2521 taaaggaagg gaattagatc atcaaacagg tcactcacac ctacctctaa cttcacacac
     2581 cttggtcaat gtgaaacaag cttgaactgg ttatatgctt ccccaccatt tttatttatt
     2641 gtaagctact ttaaaaattt tagacaatgt tgtaataaaa atatacacct ttttccccca
     2701 ttatccctgg tcttctggtg aagcaagcag tgtaatcaat gattcttatg tgtgaggagc
     2761 aactccaagt agaggcgttc atgtccctaa tgctttttta tttttcctca tcagcaaatt
     2821 taaactacaa ctttatcctg atcccactgc tgctctttgc ctcttctccc cccagccctt
     2881 ccacatttac cagcctcctc cctacctcct cccagccttt ccctttgccc tcccctttct
     2941 tctagcccct cctcccagtt gagtcagtgg cttgaaactt ttaaaagctc tgtgctccaa
     3001 gttacaaaaa agcttttacg aggtatcagc acttttcttt cattaggggg aaggcgtgag
     3061 gaaagtacca aacagcagcg gagttttaaa ctttaaatag acaggtctga gtgcctgaac
     3121 ttgccttttc attttacttc atcctccaag gagttcaatc acttggcgtg acttcactac
     3181 ttttaagcgg atctgcgatc taagtaagct tggcattccg gtactgttgg taaagccacc
     3241 atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga
     3301 accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt
     3361 gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc
     3421 gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta
     3481 tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt
     3541 gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt
     3601 tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa
     3661 aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga
     3721 tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat
     3781 tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga
     3841 tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg
     3901 catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt
     3961 gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt
     4021 cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct tcaggattac
     4081 aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa aagcactctg
     4141 attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc tcccctctct
     4201 aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag gcaaggatat
     4261 gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc
     4321 gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa
     4381 acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat tatgtccggt
     4441 tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct
     4501 ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg cctgaagtct
     4561 ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat cttgctccaa
     4621 caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt
     4681 cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat
     4741 tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac
     4801 gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata
     4861 aaggccaaga agggcggaaa gatcgccgtg taattctaga gtcggggcgg ccggccgctt
     4921 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     4981 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     5041 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     5101 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat
     5161 ccgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
     5221 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
     5281 gccggcagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     5341 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     5401 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     5461 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5521 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     5581 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     5641 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     5701 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     5761 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     5821 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     5881 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     5941 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6001 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6061 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6121 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     6181 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     6241 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     6301 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     6361 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     6421 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     6481 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     6541 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     6601 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     6661 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     6721 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     6781 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     6841 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     6901 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     6961 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7021 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7081 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7141 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     7201 catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     7261 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     7321 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     7381 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     7441 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     7501 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     7561 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     7621 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
     7681 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     7741 tattacgcca gcccaagcta ccatgataag taagtaatat taaggtacgg gaggtacttg
     7801 gagc
//
