LOCUS       V001682                 5427 bp    DNA     circular SYN 13-JAN-2022
DEFINITION  Exported.
ACCESSION   V001682
VERSION     V001682
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             21..407
                     /gene="cheY"
                     /label="Chemotaxis protein CheY"
                     /note="Chemotaxis protein CheY from Escherichia coli
                     (strain K12). Accession#: P0AE67"
     primer_bind     complement(465..481)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      662..1117
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1144..1248
                     /label="AmpR promoter"
     CDS             1249..2106
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2280..2868
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(3054..3196)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(3605..3778)
                     /label="lacZ-alpha"
                     /note="LacZ-alpha fragment of beta-galactosidase"
     protein_bind    complement(3798..3814)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3822..3852)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3867..3888)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3904..4983)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(4984..5061)
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     protein_bind    5113..5134
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5149..5179
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    5187..5203
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        5258..5286
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    5294..5310
                     /label="lac repressor encoded by lacI binding site"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    5353..5381
                     /label="tac promoter"
                     /note="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    5389..5405
                     /label="lac repressor encoded by lacI binding site"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atcgatgctt aggaggtcat atggcggata aagaacttaa atttttggtt gtggatgact
       61 tttccaccat gcgacgcata gtgcgtaacc tgctgaaaga gctgggattc aataatgttg
      121 aggaagcgga agatggcgtc gacgctctca ataagttgca ggcaggcggt tatggatttg
      181 ttatctccga ctggaacatg cccaatatgg atggcctgga attgctgaaa acaattcgtg
      241 cggatggcgc gatgtcggca ttgccagtgt taatggtgac tgcagaagcg aagaaagaga
      301 acatcattgc tgcggcgcaa gcgggggcca gtggctatgt ggtgaagcca tttaccgccg
      361 cgacgctgga ggaaaaactc aacaaaatct ttgagaaact gggcatgtga ggatgcgact
      421 atgatgcaac catcaatcaa acctgctgac gagcattcag gggggatcca ctagttctag
      481 agtcgacctg cagcccaagc ttatcgatga taagctgtca aacatgagca gatctgagcc
      541 cgcctaatga gcgggctttt ttttcagatc tgcttgaaga cgaaagggcc tcgtgatacg
      601 cctattttta taggttaatg tcatgataat aatggtttct tagcgtcaaa gcaaccatag
      661 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      721 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      781 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      841 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
      901 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      961 ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta
     1021 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     1081 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
     1141 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     1201 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     1261 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     1321 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     1381 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     1441 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     1501 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     1561 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     1621 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1681 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1741 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     1801 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1861 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1921 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1981 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     2041 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     2101 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     2161 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     2221 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2281 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2341 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2401 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     2461 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2521 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2581 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2641 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2701 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2761 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2821 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2881 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2941 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3001 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     3061 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca
     3121 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
     3181 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     3241 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     3301 gaggttttca ccgtcatcac cgaaacgcgc gaggcagaac gccatcaaaa ataattcgcg
     3361 tctggccttc ctgtagccag ctttcatcaa cattaaatgt gagcgagtaa caacccgtcg
     3421 gattctccgt gggaacaaac ggcggattga ccgtaatggg ataggttacg ttggtgtaga
     3481 tgggcgcatc gtaaccgtgc atctgccagt ttgaggggac gacgacagta tcggcctcag
     3541 gaagatcgca ctccagccag ctttccggca ccgcttctgg tgccggaaac caggcaaagc
     3601 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     3661 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     3721 ggttttccca gtcacgacgt tgtaaaacga cggccagtga atccgtaatc atggtcatag
     3781 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     3841 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     3901 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     3961 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga
     4021 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc
     4081 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata
     4141 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag
     4201 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat
     4261 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc
     4321 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg
     4381 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat
     4441 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga
     4501 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt
     4561 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag
     4621 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct
     4681 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc
     4741 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa
     4801 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat
     4861 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg
     4921 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt
     4981 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
     5041 tttgcaccat tcgatggtgt cctggcacga caggtttccc gactggaaag cgggcagtga
     5101 gcgcaacgca attaatgtaa gttagctcac tcattaggca ccccaggctt tacactttat
     5161 gcttccggct cgtataatgt gtggaattgt gagcggataa caatttcaca caggaaacag
     5221 gatcgatcca tcgatgagct tactccccat ccccctgttg acaattaatc atcggctcgt
     5281 ataatgtgtg gaattgtgag cggataacaa tttcacacag gaaacaggat cagcttactc
     5341 cccatccccc tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat
     5401 aacaatttca cacaggaaac aggatcc
//
