LOCUS       40924_13845        4893 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCV2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   VanDrisse CM, Escalante-Semerena JC.
  TITLE     New high-cloning-efficiency vectors for complementation studies and 
            recombinant protein overproduction in Escherichia coli and 
            Salmonella enterica
  JOURNAL   Plasmid (2016) In press
  PUBMED    27234933
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   VanDrisse CM, Escalante-Semerena JC.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2016) Microbiology, University of Georgia, 120 
            Cedar St., Athens, GA 30602, USA
REFERENCE   3  (bases 1 to 4893)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4893)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid 
            (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-MAR-2016) Microbiology, University of Georgia, 120 Cedar St., 
            Athens, GA 30602, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      240..326
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      418..445
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        464..555
                     /label=AmpR promoter
     CDS             556..1413
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1458..1913
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      complement(2874..3419)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(3693..4568)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4595..4879
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
ORIGIN
        1 gctagcgaat tccgaagagc gctcttcata aaagcttggc tgttttggcg gatgagagaa
       61 gattttcagc ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt
      121 gcctggcggc agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg
      181 ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc
      241 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      301 tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac
      361 ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga
      421 aggccatcct gacggatggc ctttttgcgt ttctacaaac tcttttgttt atttttctaa
      481 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      541 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      601 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      661 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      721 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      781 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
      841 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      901 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      961 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     1021 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     1081 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     1141 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     1201 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     1261 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     1321 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     1381 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1441 atactttaga ttgatttacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     1501 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     1561 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     1621 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     1681 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     1741 cacgttcttt aatagtggac tcttgttcca aacttgaaca acactcaacc ctatctcggg
     1801 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     1861 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa tttaaaagga
     1921 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     1981 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2041 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2101 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     2161 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     2221 cgcctacata cctcgctctg ctaatcctgt taccagtcag gcatttgaga agcacacggt
     2281 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     2341 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     2401 cgcttattat cacttattca ggcgtagcac caggcgttta agggcaccaa taactgcctt
     2461 aaaaaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     2521 tgccgacatg gaagccatca cagacggcat gatgaacctg aatcgccagc ggcatcagca
     2581 ccttgtcgcc ttgcgtataa tatttgccgc tagcggagtg tatactggct tactatgttg
     2641 gcactgatga gggtgtcagt gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt
     2701 gcgtcagcag aatatgtgat acaggatata ttccgcttcc tcgctcactg actcgctacg
     2761 ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa cggggcggag atttcctgga
     2821 agatgccagg aagatactta acagggaagt gagagggccg cggcaaagcc gtttttccat
     2881 aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac
     2941 ccgacaggac tataaagata ccaggcgttt ccccctggcg gctccctcgt gcgctctcct
     3001 gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc
     3061 acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc
     3121 ccccgttcag tccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga
     3181 aagacatgca aaagcaccac tggcagcagc cactggtaat tgatttagag gagttagtct
     3241 tgaagtcatg cgccggttaa ggctaaactg aaaggacaag ttttggtgac tgcgctcctc
     3301 caagccagtt acctcggttc aaagagttgg tagctcagag aaccttcgaa aaaccgccct
     3361 gcaaggcggt tttttcgttt tcagagcaag agattacgcg cagaccaaaa cgatctcaag
     3421 aagatcatct tattaatcag ataaaatatt tgctcatgag cccgaagtgg cgagcccgat
     3481 cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga
     3541 tgccggccac gatgcgtccg gcgtagagga tctgctcatg tttgacagct tatcatcgat
     3601 gcataatgtg cctgtcaaat ggacgaagca gggattctgc aaaccctatg ctactccgtc
     3661 aagccgtcaa ttgtctgatt cgttaccaat tatgacaact tgacggctac atcattcact
     3721 ttttcttcac aaccggcacg gaactcgctc gggctggccc cggtgcattt tttaaatacc
     3781 cgcgagaaat agagttgatc gtcaaaacca acattgcgac cgacggtggc gataggcatc
     3841 cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag
     3901 acgctaatcc ctaactgctg gcggaaaaga tgtgacagac gcgacggcga caagcaaaca
     3961 tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct gatgtactga
     4021 caagcctcgc gtacccgatt atccatcggt ggatggagcg actcgttaat cgcttccatg
     4081 cgccgcagta acaattgctc aagcagattt atcgccagca gctccgaata gcgcccttcc
     4141 ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca
     4201 tccgggcgaa agaaccccgt attggcaaat attgacggcc agttaagcca ttcatgccag
     4261 taggcgcgcg gacgaaagta aacccactgg tgataccatt cgcgagcctc cggatgacga
     4321 ccgtagtgat gaatctctcc tggcgggaac agcaaaatat cacccggtcg gcaaacaaat
     4381 tctcgtccct gatttttcac caccccctga ccgcgaatgg tgagattgag aatataacct
     4441 ttcattccca gcggtcggtc gataaaaaaa tcgagataac cgttggcctc aatcggcgtt
     4501 aaacccgcca ccagatgggc attaaacgag tatcccggca gcaggggatc attttgcgct
     4561 tcagccatac ttttcatact cccgccattc agagaagaaa ccaattgtcc atattgcatc
     4621 agacattgcc gtcactgcgt cttttactgg cwcwtctcgc taaccaaacc ggtaaccccg
     4681 cttattaaaa gcattctgta acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa
     4741 agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt cacactttgc
     4801 tatgccatag catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa
     4861 ctctctactg tttctccata cccgtttttt tgg
//
