LOCUS       .                       6901 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(15..33)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(40..56)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      198..653
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        679..783
                     /label="AmpR promoter"
     CDS             784..1641
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      1815..2403
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2691..2712
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2727..2757
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2765..2781
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     2789..2805
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2826..2844
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             2862..3554
                     /label="TurboRFP"
                     /note="red fluorescent protein from Entacmaea quadricolor"
     CDS             3591..5399
                     /label="GUS"
                     /note="β-glucuronidase"
     CDS             complement(5446..6468)
                     /label="HygR"
                     /note="hygromycin B phosphotransferase"
     promoter        complement(6473..6827)
                     /label="trpC promoter"
                     /note="promoter for Aspergillus nidulans trpC"
     primer_bind     complement(6867..6883)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 gagctccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg
       61 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
      121 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
      181 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
      241 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
      301 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
      361 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
      421 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
      481 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
      541 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
      601 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
      661 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      721 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      781 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      841 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      901 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
      961 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     1021 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     1081 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     1141 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1201 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1261 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1321 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1381 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     1441 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     1501 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     1561 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1621 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     1681 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     1741 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1801 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1861 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1921 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     1981 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2041 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2101 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2161 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2221 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2281 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2341 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2401 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2461 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2521 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     2581 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     2641 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     2701 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     2761 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     2821 ctcgaaatta accctcacta aagggaacaa aagctggtac catgagcgag ctgatcaagg
     2881 agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac ttcaagtgca
     2941 catccgaggg cgaaggcaag ccctacgagg gcacccagac catgaagatc aaggtggtcg
     3001 agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca
     3061 aagccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc ttccctgagg
     3121 gcttcacatg ggagagaatc accacatacg aagacggggg cgtgctgacc gctacccagg
     3181 acaccagctt ccagaacggc tgcatcatct acaacgtcaa gatcaacggg gtgaacttcc
     3241 catccaacgg ccctgtgatg cagaagaaaa cacgcggctg ggaggccaac accgagatgc
     3301 tgtaccccgc tgacggcggc ctgagaggcc acagccagat ggccctgaag ctcgtgggcg
     3361 ggggctacct gcactgctcc ttcaagacca catacagatc caagaaaccc gctaagaacc
     3421 tcaagatgcc cggcttccac ttcgtggacc acagactgga aagaatcaag gaggccgaca
     3481 aagagaccta cgtcgagcag cacgagatgg ctgtggccaa gtactgcgac ctccctagca
     3541 aactggggca cagactcgag ggcggtggag gaagcggcgg aggtggatct atgttacgtc
     3601 ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg cctgtgggca ttcagtctgg
     3661 atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag cgcgttacaa gaaagccggg
     3721 caattgctgt gccaggcagt tttaacgatc agttcgccga tgcagatatt cgtaattatg
     3781 cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa aggttgggca ggccagcgta
     3841 tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt gtgggtcaat aatcaggaag
     3901 tgatggagca tcagggcggc tatacgccat ttgaagccga tgtcacgccg tatgttattg
     3961 ccgggaaaag tgtacgtatc accgtttgtg tgaacaacga actgaactgg cagactatcc
     4021 cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa gcagtcttac ttccatgatt
     4081 tctttaacta tgccggaatc catcgcagcg taatgctcta caccacgccg aacacctggg
     4141 tggacgatat caccgtggtg acgcatgtcg cgcaagactg taaccacgcg tctgttgact
     4201 ggcaggtggt ggccaatggt gatgtcagcg ttgaactgcg tgatgcggat caacaggtgg
     4261 ttgcaactgg acaaggcact agcgggactt tgcaagtggt gaatccgcac ctctggcaac
     4321 cgggtgaagg ttatctctat gaactgtgcg tcacagccaa aagccagaca gagtgtgata
     4381 tctacccgct tcgcgtcggc atccggtcag tggcagtgaa gggcgaacag ttcctgatta
     4441 accacaaacc gttctacttt actggctttg gtcgtcatga agatgcggac ttgcgtggca
     4501 aaggattcga taacgtgctg atggtgcacg accacgcatt aatggactgg attggggcca
     4561 actcctaccg tacctcgcat tacccttacg ctgaagagat gctcgactgg gcagatgaac
     4621 atggcatcgt ggtgattgat gaaactgctg ctgtcggctt taacctctct ttaggcattg
     4681 gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga agaggcagtc aacggggaaa
     4741 ctcagcaagc gcacttacag gcgattaaag agctgatagc gcgtgacaaa aaccacccaa
     4801 gcgtggtgat gtggagtatt gccaacgaac cggatacccg tccgcaaggt gcacgggaat
     4861 atttcgcgcc actggcggaa gcaacgcgta aactcgaccc gacgcgtccg atcacctgcg
     4921 tcaatgtaat gttctgcgac gctcacaccg ataccatcag cgatctcttt gatgtgctgt
     4981 gcctgaaccg ttattacgga tggtatgtcc aaagcggcga tttggaaacg gcagagaagg
     5041 tactggaaaa agaacttctg gcctggcagg agaaactgca tcagccgatt atcatcaccg
     5101 aatacggcgt ggatacgtta gccgggctgc actcaatgta caccgacatg tggagtgaag
     5161 agtatcagtg tgcatggctg gatatgtatc accgcgtctt tgatcgcgtc agcgccgtcg
     5221 tcggtgaaca ggtatggaat ttcgccgatt ttgcgacctc gcaaggcata ttgcgcgttg
     5281 gcggtaacaa gaaagggatc ttcactcgcg accgcaaacc gaagtcggcg gcttttctgc
     5341 tgcaaaaacg ctggactggc atgaacttcg gtgaaaaacc gcagcaggga ggcaaacaat
     5401 gactcgaggt cgacgttaac tggttcccgg tcggcatcta ctctattcct ttgccctcgg
     5461 acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca
     5521 gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac
     5581 gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct
     5641 ctgatagagt tggtcaagac caatgcggag catatacgcc cggaggcgcg gcgatcctgc
     5701 aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg
     5761 cctccagaag aggatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca
     5821 gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc
     5881 atgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc
     5941 ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg
     6001 atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc
     6061 gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatggcctc
     6121 cgcgaccggc tggagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc
     6181 ctgtgcacgg cgggagatgc aataggtcag gctctcgctg aactccccaa tgtcaagcac
     6241 ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa
     6301 accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa
     6361 agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc
     6421 gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcattt ggatgcttgg
     6481 gtagaatagg taagtcagat tgaatctgaa ataaagggag gaagggcgaa cttaagaagg
     6541 tatgaccggg tcgtccactt accttgcttg acaaacgcac caagttatcg tgcaccaagc
     6601 agcagatgat aataatgtcc tcgttcctgt ctgctaataa gagtcacact tcgagcgccg
     6661 ccgctactgc tacaagtggg gctgatctga ccagttgcct aaatgaacca tcttgtcaaa
     6721 cgacacaaat tttgtgctca ccgcctggac gactaaacca aaataggcat tcattgttga
     6781 cctccactag ctccagccaa gcccaaaaaa tgctccttca atatcagtta acgtcgacgg
     6841 tatcgatgaa ttcctgcagc ccgggggatc cactagttct agagcggccg ccaccgcggt
     6901 g
//
