LOCUS       .                       6925 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(15..33)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(40..56)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      198..653
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        679..783
                     /label="AmpR promoter"
     CDS             784..1641
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      1815..2403
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2691..2712
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2727..2757
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2765..2781
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     2789..2805
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2826..2844
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             2862..3578
                     /label="EYFP"
                     /note="enhanced YFP"
     CDS             3615..5423
                     /label="GUS"
                     /note="β-glucuronidase"
     CDS             complement(5470..6492)
                     /label="HygR"
                     /note="hygromycin B phosphotransferase"
     promoter        complement(6497..6851)
                     /label="trpC promoter"
                     /note="promoter for Aspergillus nidulans trpC"
     primer_bind     complement(6891..6907)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 gagctccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg
       61 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
      121 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
      181 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
      241 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
      301 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
      361 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
      421 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
      481 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
      541 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
      601 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
      661 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      721 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      781 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      841 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      901 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
      961 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     1021 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     1081 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     1141 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1201 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1261 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1321 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1381 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     1441 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     1501 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     1561 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1621 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     1681 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     1741 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1801 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1861 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1921 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     1981 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2041 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2101 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2161 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2221 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2281 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2341 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2401 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2461 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2521 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     2581 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     2641 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     2701 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     2761 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     2821 ctcgaaatta accctcacta aagggaacaa aagctggtac catggtgagc aagggcgagg
     2881 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     2941 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     3001 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct
     3061 acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     3121 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     3181 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     3241 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     3301 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     3361 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     3421 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg
     3481 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     3541 ccgccgggat cactctcggc atggacgagc tgtacaagct cgagggcggt ggaggaagcg
     3601 gcggaggtgg atctatgtta cgtcctgtag aaaccccaac ccgtgaaatc aaaaaactcg
     3661 acggcctgtg ggcattcagt ctggatcgcg aaaactgtgg aattgatcag cgttggtggg
     3721 aaagcgcgtt acaagaaagc cgggcaattg ctgtgccagg cagttttaac gatcagttcg
     3781 ccgatgcaga tattcgtaat tatgcgggca acgtctggta tcagcgcgaa gtctttatac
     3841 cgaaaggttg ggcaggccag cgtatcgtgc tgcgtttcga tgcggtcact cattacggca
     3901 aagtgtgggt caataatcag gaagtgatgg agcatcaggg cggctatacg ccatttgaag
     3961 ccgatgtcac gccgtatgtt attgccggga aaagtgtacg tatcaccgtt tgtgtgaaca
     4021 acgaactgaa ctggcagact atcccgccgg gaatggtgat taccgacgaa aacggcaaga
     4081 aaaagcagtc ttacttccat gatttcttta actatgccgg aatccatcgc agcgtaatgc
     4141 tctacaccac gccgaacacc tgggtggacg atatcaccgt ggtgacgcat gtcgcgcaag
     4201 actgtaacca cgcgtctgtt gactggcagg tggtggccaa tggtgatgtc agcgttgaac
     4261 tgcgtgatgc ggatcaacag gtggttgcaa ctggacaagg cactagcggg actttgcaag
     4321 tggtgaatcc gcacctctgg caaccgggtg aaggttatct ctatgaactg tgcgtcacag
     4381 ccaaaagcca gacagagtgt gatatctacc cgcttcgcgt cggcatccgg tcagtggcag
     4441 tgaagggcga acagttcctg attaaccaca aaccgttcta ctttactggc tttggtcgtc
     4501 atgaagatgc ggacttgcgt ggcaaaggat tcgataacgt gctgatggtg cacgaccacg
     4561 cattaatgga ctggattggg gccaactcct accgtacctc gcattaccct tacgctgaag
     4621 agatgctcga ctgggcagat gaacatggca tcgtggtgat tgatgaaact gctgctgtcg
     4681 gctttaacct ctctttaggc attggtttcg aagcgggcaa caagccgaaa gaactgtaca
     4741 gcgaagaggc agtcaacggg gaaactcagc aagcgcactt acaggcgatt aaagagctga
     4801 tagcgcgtga caaaaaccac ccaagcgtgg tgatgtggag tattgccaac gaaccggata
     4861 cccgtccgca aggtgcacgg gaatatttcg cgccactggc ggaagcaacg cgtaaactcg
     4921 acccgacgcg tccgatcacc tgcgtcaatg taatgttctg cgacgctcac accgatacca
     4981 tcagcgatct ctttgatgtg ctgtgcctga accgttatta cggatggtat gtccaaagcg
     5041 gcgatttgga aacggcagag aaggtactgg aaaaagaact tctggcctgg caggagaaac
     5101 tgcatcagcc gattatcatc accgaatacg gcgtggatac gttagccggg ctgcactcaa
     5161 tgtacaccga catgtggagt gaagagtatc agtgtgcatg gctggatatg tatcaccgcg
     5221 tctttgatcg cgtcagcgcc gtcgtcggtg aacaggtatg gaatttcgcc gattttgcga
     5281 cctcgcaagg catattgcgc gttggcggta acaagaaagg gatcttcact cgcgaccgca
     5341 aaccgaagtc ggcggctttt ctgctgcaaa aacgctggac tggcatgaac ttcggtgaaa
     5401 aaccgcagca gggaggcaaa caatgactcg aggtcgacgt taactggttc ccggtcggca
     5461 tctactctat tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag
     5521 tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc
     5581 gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc
     5641 atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata
     5701 cgcccggagg cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg
     5761 ctgctccata caagccaacc acggcctcca gaagaggatg ttggcgacct cgtattggga
     5821 atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag
     5881 gacattgttg gagccgaaat ccgcatgcac gaggtgccgg acttcggggc agtcctcggc
     5941 ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac
     6001 agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt
     6061 gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc
     6121 cgcagcgatc gcatccatgg cctccgcgac cggctggaga acagcgggca gttcggtttc
     6181 aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc
     6241 gctgaactcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg
     6301 ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc
     6361 acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag
     6421 gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc
     6481 aggctttttc atttggatgc ttgggtagaa taggtaagtc agattgaatc tgaaataaag
     6541 ggaggaaggg cgaacttaag aaggtatgac cgggtcgtcc acttaccttg cttgacaaac
     6601 gcaccaagtt atcgtgcacc aagcagcaga tgataataat gtcctcgttc ctgtctgcta
     6661 ataagagtca cacttcgagc gccgccgcta ctgctacaag tggggctgat ctgaccagtt
     6721 gcctaaatga accatcttgt caaacgacac aaattttgtg ctcaccgcct ggacgactaa
     6781 accaaaatag gcattcattg ttgacctcca ctagctccag ccaagcccaa aaaatgctcc
     6841 ttcaatatca gttaacgtcg acggtatcga tgaattcctg cagcccgggg gatccactag
     6901 ttctagagcg gccgccaccg cggtg
//
