LOCUS       .                       7629 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(15..33)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(40..56)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      198..653
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        679..783
                     /label="AmpR promoter"
     CDS             784..1641
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      1815..2403
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2691..2712
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2727..2757
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2765..2781
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     2789..2805
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2826..2844
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(2914..3936)
                     /label="HygR"
                     /note="hygromycin B phosphotransferase"
     promoter        complement(3941..4295)
                     /label="trpC promoter"
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             4723..5439
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     CDS             5470..7278
                     /label="GUS"
                     /note="β-glucuronidase"
     terminator      7300..7552
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(7595..7611)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 gagctccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg
       61 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
      121 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
      181 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
      241 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
      301 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
      361 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
      421 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
      481 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
      541 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
      601 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
      661 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      721 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      781 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      841 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      901 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
      961 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     1021 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     1081 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     1141 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1201 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1261 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1321 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1381 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     1441 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     1501 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     1561 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1621 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     1681 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     1741 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1801 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1861 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1921 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     1981 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2041 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2101 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2161 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2221 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2281 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2341 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2401 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2461 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2521 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     2581 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     2641 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     2701 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     2761 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     2821 ctcgaaatta accctcacta aagggaacaa aagctggtac cgggcccccc ctcgaggtcg
     2881 acgttaactg gttcccggtc ggcatctact ctattccttt gccctcggac gagtgctggg
     2941 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
     3001 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
     3061 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
     3121 gtcaagacca atgcggagca tatacgcccg gaggcgcggc gatcctgcaa gctccggatg
     3181 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagag
     3241 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
     3301 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcat gcacgaggtg
     3361 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
     3421 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
     3481 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
     3541 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg cgaccggctg
     3601 gagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg
     3661 ggagatgcaa taggtcaggc tctcgctgaa ctccccaatg tcaagcactt ccggaatcgg
     3721 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca
     3781 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc
     3841 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
     3901 ctcgacagac gtcgcggtga gttcaggctt tttcatttgg atgcttgggt agaataggta
     3961 agtcagattg aatctgaaat aaagggagga agggcgaact taagaaggta tgaccgggtc
     4021 gtccacttac cttgcttgac aaacgcacca agttatcgtg caccaagcag cagatgataa
     4081 taatgtcctc gttcctgtct gctaataaga gtcacacttc gagcgccgcc gctactgcta
     4141 caagtggggc tgatctgacc agttgcctaa atgaaccatc ttgtcaaacg acacaaattt
     4201 tgtgctcacc gcctggacga ctaaaccaaa ataggcattc attgttgacc tccactagct
     4261 ccagccaagc ccaaaaaatg ctccttcaat atcagttaac gtcgacggta tcgattggaa
     4321 tgcatggagg agttctgtac gcgcaattcc gctctccgta aggatgcttc ggaggtgcac
     4381 atggtctcat acatgtaggc ccgacgagga tcgagtcggt tccgaagtag gatcgtctcg
     4441 attgttgggc atcattgcat ggacattcag agggcctact gatacctgga atccgcaccg
     4501 tccggctacc tagcaataag attctgtgta tataaagggc taaggtgtcc gtccttgata
     4561 aaaccaccac cctcaacaac ttacctcgac tatcagcatc ccgtcctatc taacaatcgt
     4621 ccatcggtat ccaactccaa ctctattcgc agggtcctag aatcgtaagt acacgcttat
     4681 atcttgttgc cagcgatagc tgacaatgaa tgaatatagg ccatggtgag caagggcgag
     4741 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     4801 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     4861 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcacc
     4921 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     4981 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     5041 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     5101 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     5161 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     5221 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     5281 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     5341 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     5401 gccgccggga tcactcacgg catggacgag ctgtacaagg gcggtggagg aagcggcgga
     5461 ggtggatcta tgttacgtcc tgtagaaacc ccaacccgtg aaatcaaaaa actcgacggc
     5521 ctgtgggcat tcagtctgga tcgcgaaaac tgtggaattg atcagcgttg gtgggaaagc
     5581 gcgttacaag aaagccgggc aattgctgtg ccaggcagtt ttaacgatca gttcgccgat
     5641 gcagatattc gtaattatgc gggcaacgtc tggtatcagc gcgaagtctt tataccgaaa
     5701 ggttgggcag gccagcgtat cgtgctgcgt ttcgatgcgg tcactcatta cggcaaagtg
     5761 tgggtcaata atcaggaagt gatggagcat cagggcggct atacgccatt tgaagccgat
     5821 gtcacgccgt atgttattgc cgggaaaagt gtacgtatca ccgtttgtgt gaacaacgaa
     5881 ctgaactggc agactatccc gccgggaatg gtgattaccg acgaaaacgg caagaaaaag
     5941 cagtcttact tccatgattt ctttaactat gccggaatcc atcgcagcgt aatgctctac
     6001 accacgccga acacctgggt ggacgatatc accgtggtga cgcatgtcgc gcaagactgt
     6061 aaccacgcgt ctgttgactg gcaggtggtg gccaatggtg atgtcagcgt tgaactgcgt
     6121 gatgcggatc aacaggtggt tgcaactgga caaggcacta gcgggacttt gcaagtggtg
     6181 aatccgcacc tctggcaacc gggtgaaggt tatctctatg aactgtgcgt cacagccaaa
     6241 agccagacag agtgtgatat ctacccgctt cgcgtcggca tccggtcagt ggcagtgaag
     6301 ggcgaacagt tcctgattaa ccacaaaccg ttctacttta ctggctttgg tcgtcatgaa
     6361 gatgcggact tgcgtggcaa aggattcgat aacgtgctga tggtgcacga ccacgcatta
     6421 atggactgga ttggggccaa ctcctaccgt acctcgcatt acccttacgc tgaagagatg
     6481 ctcgactggg cagatgaaca tggcatcgtg gtgattgatg aaactgctgc tgtcggcttt
     6541 aacctctctt taggcattgg tttcgaagcg ggcaacaagc cgaaagaact gtacagcgaa
     6601 gaggcagtca acggggaaac tcagcaagcg cacttacagg cgattaaaga gctgatagcg
     6661 cgtgacaaaa accacccaag cgtggtgatg tggagtattg ccaacgaacc ggatacccgt
     6721 ccgcaaggtg cacgggaata tttcgcgcca ctggcggaag caacgcgtaa actcgacccg
     6781 acgcgtccga tcacctgcgt caatgtaatg ttctgcgacg ctcacaccga taccatcagc
     6841 gatctctttg atgtgctgtg cctgaaccgt tattacggat ggtatgtcca aagcggcgat
     6901 ttggaaacgg cagagaaggt actggaaaaa gaacttctgg cctggcagga gaaactgcat
     6961 cagccgatta tcatcaccga atacggcgtg gatacgttag ccgggctgca ctcaatgtac
     7021 accgacatgt ggagtgaaga gtatcagtgt gcatggctgg atatgtatca ccgcgtcttt
     7081 gatcgcgtca gcgccgtcgt cggtgaacag gtatggaatt tcgccgattt tgcgacctcg
     7141 caaggcatat tgcgcgttgg cggtaacaag aaagggatct tcactcgcga ccgcaaaccg
     7201 aagtcggcgg cttttctgct gcaaaaacgc tggactggca tgaacttcgg tgaaaaaccg
     7261 cagcagggag gcaaacaatg aagcggccgc ccggctgcag atcgttcaaa catttggcaa
     7321 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     7381 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     7441 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     7501 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tccgatgata
     7561 agctgtcaaa catgagaatt cctgcagccc gggggatcca ctagttctag agcggccgcc
     7621 accgcggtg
//
