LOCUS       40924_13720        4398 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCST5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Cote P, Sulea T, Dignard D, Wu C, Whiteway M.
  TITLE     Evolutionary reshaping of fungal mating pathway scaffold proteins
  JOURNAL   MBio 2 (1), e00230-10 (2011)
  PUBMED    21249169
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Cote P.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2010) Health Sector, CNRC-BRI, 6100 Royalmount 
            Av., Montreal, QC H4P2R2, Canada
REFERENCE   3  (bases 1 to 4398)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4398)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio"; 
            date: "2011"; volume: "2"; issue: "1"; pages: "e00230-10"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-NOV-2010) Health Sector, CNRC-BRI, 6100 Royalmount Av., 
            Montreal, QC H4P2R2, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             411..1562
                     /codon_start=1
                     /gene="CST5"
                     /product="Cst5"
                     /label=CST5
                     /note="ORF19.2127; similar to Candida albicans STE5"
                     /protein_id="ADW40657.1"
                     /translation="MLQSTPKSWKDFLKSPKKPTSPTTPPGSVTITPNTTPPSVPCVSP
                     RPAKIPSRDPFQTINPGLNLSFANILNKSLANHETCFICGELLSTVFASERILKLNCGD
                     STHSECFKAYFKEDIPHARIHGKTITFAKTCRGLNCSGTRNVVVDVNNWHLVSARKLSL
                     IPKRPAPPHPNSVNINALKTTLQNSDIPTRSPSPDPTVSTTTEVDAYEVETVRNQLIKY
                     LLDLCPKINLSRLVLLGNLRVADELSVCVEPLDYFQTRYVYLFENYMVIWNSVDYPVFV
                     PMQNIQISSRGSSILQVRQKDDGLSTLIQSTSSTVVEKWVVAISDAHLQLPAPDITSTI
                     DTSPSDSDSDIDSDEEVIQQALTKNNWADLMIEIDNALLTSDP"
     gene            411..1562
                     /gene="CST5"
                     /label=CST5
     primer_bind     complement(2160..2176)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2213..2231)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2252..2268)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2276..2292)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2300..2330)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2345..2366)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2654..3242)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3416..4273)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4274..4378)
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gcctaaatct gaaacatcga ctataaaacc
      361 accaaaaaaa tcccatccat cgtcaatctg ttaatattac ccaccaggca atgctacaat
      421 ctacgcctaa gagctggaaa gactttttaa aatctccaaa gaagcccact agccctacca
      481 ccccaccagg cagtgtgacg atcacaccca atacaacacc tccaagtgtg ccatgtgtca
      541 gtccacgacc agcgaaaatc ccttccagag acccatttca aaccataaat cccggactaa
      601 acctttcctt tgccaatatt ctcaacaagt cactagcaaa tcacgaaaca tgtttcatct
      661 gtggagaact actttctact gtatttgcat cagaacgaat cctaaagtta aattgtggtg
      721 actccaccca cagtgaatgt ttcaaagctt attttaagga agatatacca cacgcaagaa
      781 tccatggcaa gacaataacg tttgccaaaa cctgtcgtgg tttaaattgc agtggcacca
      841 gaaacgtagt tgtcgatgta aacaactggc acttggtttc tgctagaaag ttgtcgttaa
      901 tacccaagag accagcacct ccacacccca acagtgtcaa cattaacgct ttaaagacaa
      961 ccttacaaaa cagcgatata cctaccagat ctccttcacc agacccaact gtttcaacca
     1021 ccacagaagt ggacgcatat gaggttgaaa cggttagaaa ccaactaatc aaatatttgt
     1081 tagatctgtg tcctaaaatc aacttgtcaa gattggttct gttgggaaat ttgcgagttg
     1141 cagacgagct atctgtttgt gtggagccac tggactattt ccaaacaagg tatgtttatt
     1201 tattcgaaaa ctacatggtt atatggaata gtgttgacta ccctgtattc gtccctatgc
     1261 agaatatcca aatctcctct cgtggatcgt ccatcctaca agtgcgtcaa aaagatgatg
     1321 ggttatcgac attgattcaa tctacatcta gcacagtagt ggaaaaatgg gttgttgcca
     1381 tttctgacgc tcatttacag ttgccagcac cggatataac gtctacaatt gacacctccc
     1441 ctagtgatag tgattccgat attgattcag acgaagaagt tattcagcaa gcactaacta
     1501 aaaacaattg ggccgattta atgattgaga ttgataatgc gttacttaca tctgacccgt
     1561 aagccgatcc atctcactta atatacacat tgtccatcga tctctttctt gtcgactatt
     1621 agctgtaaac ataatactct ttcccgttat tcccaacttt ctaatcattg ttaccattcc
     1681 aggatttttt tcattctgta cccccacttc caacagtttt ggaagctgtt tttcgtaatg
     1741 acttatgttt ctctttttgc caaaccataa tgtgaaacat aattcacaat cttcttccat
     1801 ggatttccag ccatcagggt agactcgtgg gactgaatgg gtatcgacct gtgttgttcg
     1861 agtttcacaa ttcaacaagt ctagttctgt taatttacca gtgtatacat agcattgtga
     1921 caatggaata gtaatataat gctcataaaa tgctgtcagt ttcggagttc ctgtccccct
     1981 ggacctccta aaggtgtggt agataatcaa atacccagga catagcacaa cataaaagtg
     2041 attgaaacta gcgtgttttt tattcttctc gtacaaatac ccactcttaa tcaccaacat
     2101 attaggtagt agatgcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
     2161 atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
     2221 gagggttaat ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     2281 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     2341 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     2401 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     2461 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     2521 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     2581 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     2641 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2701 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2761 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2821 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     2881 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2941 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3001 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3061 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     3121 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3181 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3241 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     3301 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     3361 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     3421 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     3481 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     3541 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     3601 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     3661 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     3721 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     3781 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     3841 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     3901 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     3961 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     4021 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     4081 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     4141 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4201 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     4261 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     4321 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     4381 catttccccg aaaagtgc
//
