LOCUS       40924_13705        5938 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCST5-del-SAT1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Cote P, Sulea T, Dignard D, Wu C, Whiteway M.
  TITLE     Evolutionary reshaping of fungal mating pathway scaffold proteins
  JOURNAL   MBio 2 (1), e00230-10 (2011)
  PUBMED    21249169
REFERENCE   2  (bases 1 to 5938)
  AUTHORS   Cote P.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2010) Health Sector, CNRC-BRI, 6100 Royalmount 
            Av., Montreal, QC H4P2R2, Canada
REFERENCE   3  (bases 1 to 5938)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5938)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio"; 
            date: "2011"; volume: "2"; issue: "1"; pages: "e00230-10"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-NOV-2010) Health Sector, CNRC-BRI, 6100 Royalmount Av., 
            Montreal, QC H4P2R2, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             411..987
                     /codon_start=1
                     /product="Cst5"
                     /label=Cst5
                     /note="5' end disruption cassette"
                     /protein_id="ADW40662.1"
                     /translation="MLQSTPKSWKDFLKSPKKPTSPTTPPGSVTITPNTTPPSVPCVSP
                     RPAKIPSRDPFQTINPGLNLSFANILNKSLANHETCFICGELLSTVFASERILKLNCGD
                     STHSECFKAYFKEDIPHARIHGKTITFAKTCRGLNCSGTRNVVVDVNNWHLVSARKLSL
                     IPKRPAPPHPNSVNINALKTTLQNSDIPT"
     protein_bind    993..1026
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     CDS             complement(join(1164..1727,2382..2390))
                     /codon_start=1
                     /gene="SAT1"
                     /product="Sat1"
                     /label=SAT1
                     /note="norseotricin resistance protein"
                     /protein_id="ADW40661.1"
                     /translation="MDGEEVAALVIDNGSHMKISVIPEQVAETLDAENHFIVREVFDVH
                     LSDQGFELSTRSVSPYRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEH
                     IVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDL
                     FTYKTRPQVSNETAMYWYWFSGAQDDA"
     gene            complement(1164..2390)
                     /gene="SAT1"
                     /label=SAT1
     CDS             2623..3102
                     /codon_start=1
                     /product="Cst5"
                     /label=Cst5
                     /note="3' end disruption cassette with SAT1"
                     /protein_id="ADW40663.1"
                     /translation="DLCPKINLSRLVLLGNLRVADELSVCVEPLDYFQTRYVYLFENYM
                     VIWNSVDYPVFVPMQNIQISSRGSSILQVRQKDDGLSTLIQSTSSTVVEKWVVAISDAH
                     LQLPAPDITSTIDTSPSDSDSDIDSDEEVIQQALTKNNWADLMIEIDNALLTSDP"
     primer_bind     complement(3700..3716)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(3753..3771)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3792..3808)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3816..3832)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3840..3870)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3885..3906)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4194..4782)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4956..5813)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5814..5918)
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gcctaaatct gaaacatcga ctataaaacc
      361 accaaaaaaa tcccatccat cgtcaatctg ttaatattac ccaccaggca atgctacaat
      421 ctacgcctaa gagctggaaa gactttttaa aatctccaaa gaagcccact agccctacca
      481 ccccaccagg cagtgtgacg atcacaccca atacaacacc tccaagtgtg ccatgtgtca
      541 gtccacgacc agcgaaaatc ccttccagag acccatttca aaccataaat cccggactaa
      601 acctttcctt tgccaatatt ctcaacaagt cactagcaaa tcacgaaaca tgtttcatct
      661 gtggagaact actttctact gtatttgcat cagaacgaat cctaaagtta aattgtggtg
      721 actccaccca cagtgaatgt ttcaaagctt attttaagga agatatacca cacgcaagaa
      781 tccatggcaa gacaataacg tttgccaaaa cctgtcgtgg tttaaattgc agtggcacca
      841 gaaacgtagt tgtcgatgta aacaactggc acttggtttc tgctagaaag ttgtcgttaa
      901 tacccaagag accagcacct ccacacccca acagtgtcaa cattaacgct ttaaagacaa
      961 ccttacaaaa cagcgatata cctaccagat ctgaagttcc tattctctag aaagtatagg
     1021 aacttcctgc aggaccacct ttgattgtaa atagtaataa ttaccaccct tatctaatta
     1081 tttatttaac ttatttattt atttattata catatataca aatctaataa agtgaaaatc
     1141 tcccccttca cacttcacat atgttaggcg tcatcctgtg ctcccgagaa ccagtaccag
     1201 tacatcgctg tttcgttcga gacttgaggt ctagttttat acgtgaagag gtcaatgccg
     1261 ccgagagtaa agccacattt tgcgtacaaa ttgcaggcag gtacattgtt cgtttgtgtc
     1321 tctaatcgta tgccaaggag ctgtctgctt agtgcccact ttttcgcaaa ttcgatgaga
     1381 ctgtgcgcga ctcctttgcc tcggtgcgtg tgcgacacaa caatgtgttc gatagaggct
     1441 agatcgttcc atgttgagtt gagttcaatc ttcccgacaa gctcttggtc gatgaatgcg
     1501 ccatagcaag cagagtcttc atcagagtca tcatccgaga tgtaatcctt ccggtagggg
     1561 ctcacacttc tggtagatag ttcaaagcct tggtcggata ggtgcacatc gaacacttca
     1621 cgaacaatga aatggttctc agcatccaat gtttccgcca cctgctcagg gatcaccgaa
     1681 attttcatat gagaaccgtt atcgataact aaagcagcaa cttcttctat aaaaatgggt
     1741 tagtatgaca gtcatttaaa taaggaattt ttcagttggc ttggtttcaa ttcaatgttc
     1801 gttttttttt tttcttgctg tgtttgtgtt tgtgttgttt atagttgtgt gcactgagcg
     1861 tcgaaaaaaa aaattcatag tgagccggga aatctgtata gcccagataa caacacaagt
     1921 ccaaactaga aactcgtcaa acaccaaaag caatgttgaa tcaattgcct tgcacaagta
     1981 cacgtaggaa aacataaaac attgcaattt tgaatattga gccttttgtc gtaacattga
     2041 ttgataggat tactcaccga atggttttga aaccactgcc gacagatcaa tcaatcaatc
     2101 aaaaaacgtg aactttgaaa aaggggaaga acagatacat tgaagttagc catttccatt
     2161 gatcgtcaca acatatctga taaattactt tcaaaattat aagctgatgt gtgtgtatta
     2221 ttaatgtgac agtaacatcc caaacgagaa atattatgtc gacaacaaaa aagtttgatc
     2281 tgaattgaaa atgaagtttt cccaccctac ccatttgtca tattgaaacc aatcaactga
     2341 ttaatcaatc aattagaatt gaagctaaac taaaacatac caccgtccat tttgaatgat
     2401 tatatttttt taatattaat atcgagataa tgtttctaag aaagaaagaa aaccaggagt
     2461 gaaaattaga aaaggaaagg aaaggaaaaa aagaaaaatc tgaaaatata taaaaaaaaa
     2521 ttgtttcgtt ggcaataaat cttggtgaga acagcgaccg aaagcaaata agaacaaaat
     2581 atgagtgtat tacgttgaac aactaattaa cgtgtgtgta tggatctgtg tcctaaaatc
     2641 aacttgtcaa gattggttct gttgggaaat ttgcgagttg cagacgagct atctgtttgt
     2701 gtggagccac tggactattt ccaaacaagg tatgtttatt tattcgaaaa ctacatggtt
     2761 atatggaata gtgttgacta ccctgtattc gtccctatgc agaatatcca aatctcctct
     2821 cgtggatcgt ccatcctaca agtgcgtcaa aaagatgatg ggttatcgac attgattcaa
     2881 tctacatcta gcacagtagt ggaaaaatgg gttgttgcca tttctgacgc tcatttacag
     2941 ttgccagcac cggatataac gtctacaatt gacacctccc ctagtgatag tgattccgat
     3001 attgattcag acgaagaagt tattcagcaa gcactaacta aaaacaattg ggccgattta
     3061 atgattgaga ttgataatgc gttacttaca tctgacccgt aagccgatcc atctcactta
     3121 atatacacat tgtccatcga tctctttctt gtcgactatt agctgtaaac ataatactct
     3181 ttcccgttat tcccaacttt ctaatcattg ttaccattcc aggatttttt tcattctgta
     3241 cccccacttc caacagtttt ggaagctgtt tttcgtaatg acttatgttt ctctttttgc
     3301 caaaccataa tgtgaaacat aattcacaat cttcttccat ggatttccag ccatcagggt
     3361 agactcgtgg gactgaatgg gtatcgacct gtgttgttcg agtttcacaa ttcaacaagt
     3421 ctagttctgt taatttacca gtgtatacat agcattgtga caatggaata gtaatataat
     3481 gctcataaaa tgctgtcagt ttcggagttc ctgtccccct ggacctccta aaggtgtggt
     3541 agataatcaa atacccagga catagcacaa cataaaagtg attgaaacta gcgtgttttt
     3601 tattcttctc gtacaaatac ccactcttaa tcaccaacat attaggtagt agatgcgacg
     3661 gtatcgataa gcttgatatc gaattcctgc agcccggggg atccactagt tctagagcgg
     3721 ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat ttcgagcttg
     3781 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     3841 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     3901 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     3961 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     4021 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     4081 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     4141 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4201 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     4261 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4321 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4381 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4441 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4501 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     4561 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     4621 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     4681 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     4741 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     4801 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     4861 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     4921 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     4981 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5041 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     5101 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5161 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5221 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     5281 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     5341 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     5401 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     5461 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     5521 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     5581 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     5641 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     5701 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     5761 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     5821 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     5881 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgc
//
