LOCUS       V008130                 6220 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008130
VERSION     V008130
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Cote P, Sulea T, Dignard D, Wu C, Whiteway M.
  TITLE     Evolutionary reshaping of fungal mating pathway scaffold proteins
  JOURNAL   MBio 2 (1), e00230-10 (2011)
   PUBMED   21249169
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   Cote P.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2010) Health Sector, CNRC-BRI, 6100 Royalmount
            Av., Montreal, QC H4P2R2, Canada
REFERENCE   3  (bases 1 to 6220)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6220)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio";
            date: "2011"; volume: "2"; issue: "1"; pages: "e00230-10"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-NOV-2010) Health Sector, CNRC-BRI, 6100 Royalmount Av.,
            Montreal, QC H4P2R2, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        626..644
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1124..1933
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     primer_bind     2060..2076
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2746..3897
                     /codon_start=1
                     /product="Cst5"
                     /label="Cst5"
                     /note="similar to Candida albicans STE5"
                     /protein_id="ADW40664.1"
                     /translation="MLQSTPKSWKDFLKSPKKPTSPTTPPGSVTITPNTTPPSVPCVSP
                     RPAKIPSRDPFQTINPGLNLSFANILNKSLANHETCFICGELLSTVFASERILKLNCGD
                     STHSECFKAYFKEDIPHARIHGKTITFAKTCRGLNCSGTRNVVVDVNNWHLVSARKLSL
                     IPKRPAPPHPNSVNINALKTTLQNSDIPTRSPSPDPTVSTTTEVDAYEVETVRNQLIKY
                     LLDLCPKINLSRLVLLGNLRVADELSVCVEPLDYFQTRYVYLFENYMVIWNSVDYPVFV
                     PMQNIQISSRGSSILQVRQKDDGLSTLIQSTSSTVVEKWVVAISDAHLQLPAPDITSTI
                     DTSPSDSDSDIDSDEEVIQQALTKNNWADLMIEIDNALLTSDP"
     promoter        complement(3979..3997)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4018..4034)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4042..4058)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4066..4096)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4111..4132)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4420..5008)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(5727..6105)
                     /label="ISS"
                     /note="immunostimulatory sequence from the AmpR gene;
                     contains unmethylated CpG dinucleotides in the context of
                     5'-AACGTT-3' (Sato et al., 1996)"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgcggcc gacttggcca agcctagatc ccgactaata ggaattgatt
      721 tggatggtat aaacggaaac aaaaaaaaga gctggtacta ctttctttaa aattatttta
      781 ttatttgatt ttatttaata gtatatatta tattttgaac gtagattatt ttgttgaaag
      841 ttgctgtagt gccattgatt cgtaacacta attctgtatt agtcattcct cttgtttgat
      901 agtatccaaa aaaacggcta tttttttgca atcttatttc ctgcatatta tacagataac
      961 ataatgaaag aaaaaatctt tttttttgtt cttcaatgat gatttcaacc attcttttaa
     1021 acattgatca attcctgagc aacaacccca tacacactgg tttatatacc gcccctttta
     1081 cagttgaaga aagaaataga aatagaaata gcaaacaaaa gatatgacag tcaacactaa
     1141 gacctatagt gagagagcag aaactcatgc ctcaccagta gcacagcgat tatttcgatt
     1201 aatggaactg aagaaaacca atttatgtgc atcaattgac gttgatacca ctaaggaatt
     1261 ccttgaatta attgataaat taggtcctta tgtatgctta atcaagactc atattgatat
     1321 aatcaatgat ttttcctatg aatccactat tgaaccatta ttagaacttt cacgtaaaca
     1381 tcaatttatg atttttgaag atagaaaatt tgctgatatt ggtaataccg taaagaaaca
     1441 atatattggt ggagtttata aaattagtag ttgggcagat attaccaatg ctcatggtgt
     1501 cactgggaat ggagtggttg aaggattaaa acagggagct aaagaaacca ccaccaacca
     1561 agagccaaga gggttattga tgttagctga attatcatca gtgggatcat tagcatatgg
     1621 agaatattct caaaaaactg ttgaaattgc taaatccgat aaggaatttg ttattggatt
     1681 tattgcccaa cgtgatatgg gtggccaaga agaaggattt gattggctta ttatgacacc
     1741 tggagttgga ttagatgata aaggtgatgg attaggacaa caatatagaa ctgttgatga
     1801 agttgttagc actggaactg atattatcat tgttggtaga ggattgtttg gtaaaggaag
     1861 agatccagat attgaaggta aaaggtatag aaatgctggt tggaatgctt atttgaaaaa
     1921 gactggccaa ttataaatgt gaagggggag attttcactt tattagattt gtatatatgt
     1981 agaataaata aataaataag ttaaataaat aattaaataa gggtggtaat tattactatt
     2041 tacaatcaaa ggtggtcctt ctagaactag tggatccccc agatcattat ccttaaacag
     2101 attctaaaac aacatctttg aaaccagaag aaactttctt acctttttct tcggttgaac
     2161 cttcaccgtg caaagccaat aatgaacccc agtcgaattt tggttgtttc aacaatttga
     2221 cttttctgat gtggacattt tgtaatggga aaatggtttg gggggatttt tcaatttcac
     2281 ggccaatgac ttctggaatc aatttggaag ttaattgagc taaagtcagt tggaaacttc
     2341 tctttgcata atttcaatca tcttctttct gacttctctc aatttagagg attgagcgta
     2401 agtagtttcc ttgatttggt ttggttgtct tttggtgaaa gcaatggcaa aaactctcaa
     2461 aacgtaatcg tcggaagttt taacagtgac attagcttca actaatgatt gccattttct
     2521 gaccaatgat cttaatttgt cagaagtgaa atccaaacca tgagggtgaa ccatcaccct
     2581 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     2641 cccgatttag agcttgacgg ggaaagccta aatctgaaac atcgactata aaaccaccaa
     2701 aaaaatccca tccatcgtca atctgttaat attacccacc aggcaatgct acaatctacg
     2761 cctaagagct ggaaagactt tttaaaatct ccaaagaagc ccactagccc taccacccca
     2821 ccaggcagtg tgacgatcac acccaataca acacctccaa gtgtgccatg tgtcagtcca
     2881 cgaccagcga aaatcccttc cagagaccca tttcaaacca taaatcccgg actaaacctt
     2941 tcctttgcca atattctcaa caagtcacta gcaaatcacg aaacatgttt catctgtgga
     3001 gaactacttt ctactgtatt tgcatcagaa cgaatcctaa agttaaattg tggtgactcc
     3061 acccacagtg aatgtttcaa agcttatttt aaggaagata taccacacgc aagaatccat
     3121 ggcaagacaa taacgtttgc caaaacctgt cgtggtttaa attgcagtgg caccagaaac
     3181 gtagttgtcg atgtaaacaa ctggcacttg gtttctgcta gaaagttgtc gttaataccc
     3241 aagagaccag cacctccaca ccccaacagt gtcaacatta acgctttaaa gacaacctta
     3301 caaaacagcg atatacctac cagatctcct tcaccagacc caactgtttc aaccaccaca
     3361 gaagtggacg catatgaggt tgaaacggtt agaaaccaac taatcaaata tttgttagat
     3421 ctgtgtccta aaatcaactt gtcaagattg gttctgttgg gaaatttgcg agttgcagac
     3481 gagctatctg tttgtgtgga gccactggac tatttccaaa caaggtatgt ttatttattc
     3541 gaaaactaca tggttatatg gaatagtgtt gactaccctg tattcgtccc tatgcagaat
     3601 atccaaatct cctctcgtgg atcgtccatc ctacaagtgc gtcaaaaaga tgatgggtta
     3661 tcgacattga ttcaatctac atctagcaca gtagtggaaa aatgggttgt tgccatttct
     3721 gacgctcatt tacagttgcc agcaccggat ataacgtcta caattgacac ctcccctagt
     3781 gatagtgatt ccgatattga ttcagacgaa gaagttattc agcaagcact aactaaaaac
     3841 aattgggccg atttaatgat tgagattgat aatgcgttac ttacatctga cccgtaagcc
     3901 gatccatctc acttaatata cacattgtcc atcgatctct ttcttgtcga gggggggccc
     3961 ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
     4021 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     4081 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     4141 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     4201 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     4261 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4321 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4381 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4441 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4501 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4561 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4621 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4681 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4741 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4801 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4861 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4921 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4981 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     5041 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     5101 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     5161 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5221 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5281 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5341 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     5401 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5461 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     5521 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5581 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5641 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5701 atccgtaaga tgcttttctg ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     5761 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     5821 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     5881 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     5941 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     6001 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     6061 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     6121 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     6181 gatcttcagc atcttttact ttcaccagcg tttctgggtg
//
