LOCUS       40924_13695        5887 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Knock-in vector pCSR1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Bardiya N, Shiu PK.
  TITLE     Cyclosporin A-resistance based gene placement system for Neurospora 
            crassa
  JOURNAL   Fungal Genet. Biol. 44 (5), 307-314 (2007)
  PUBMED    17320431
REFERENCE   2  (bases 1 to 5887)
  AUTHORS   Bardiya N, Shiu PKT.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2006) Biological Sciences, University of Missouri,
            103 Tucker Hall, Columbia, MO 65211, USA
REFERENCE   3  (bases 1 to 5887)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5887)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet. Biol."; date: "2007"; volume: "44"; issue: "5"; pages: 
            "307-314"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-AUG-2006) Biological Sciences, University of Missouri, 103 
            Tucker Hall, Columbia, MO 65211, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(659..1680)
                     /label=csr-1 3' flank
                     /note="csr-1 3' flank"
     primer_bind     1697..1713
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1747..1763)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(1775..3674)
                     /label=csr-1 5' flank
                     /note="csr-1 5' flank"
     promoter        complement(3699..3717)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3738..3754)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3762..3778)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3786..3816)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3831..3852)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4140..4728)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4902..5759)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5760..5864)
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcga
      661 cacggccttc atcaacgagc tacaggaaat tgtgtttgac tttatcaagc agaaatcgac
      721 atatacttcc cacggcggcg gtgctggagt accaggagta ggagcacaag gaggaggagc
      781 acagcagcgt gaaaagaatc ccaagaaggg cgtggttcag gggaccattc cccactcgga
      841 ggatatttcc aaggggacga agcgcagcgc gggcgagatt tccaacgacg acacggcggc
      901 accggcaccg gccaagaaga ccaggcccgg caagccgcaa ttgctgcctc tcccggccgg
      961 ttacaagtcg tacccgactg tgtcggacat tgcaaagttc atccaccacg ccggcatcac
     1021 gaataatacg acgttgagcg aggcggatat ccagcagttg gtggatgtgc tatactacga
     1081 tggcttagta gagcctgtca aggtcaatgg gcgcaagggg taccgtgtga cgcgcatccc
     1141 caaacaggat ccgaggacgt gcaatcaacg acgcctcgac catgatccaa ccaaaggagg
     1201 cattgttacc tcatttatgg gagttgagcc gagtgcaagt ggccttatgg aggcgccttg
     1261 tgggcggtgc cctgtctatg atatgtgcga ggaaggcgga cctgtcagcc ccagcaactg
     1321 cgtctatttc agacggtggc tggatggtga cgaggtacag gaacaagtca tttcgatctg
     1381 atcgactcga atgtgtttgt gtctccgttt agaagacgtt gcatggtctc tatcacaact
     1441 gcattaacgg cgtaaaaaaa aagaaggcaa gggtaatttg gtttcatcta ggcgttggag
     1501 gtaatctgtt tagcattcaa tgtgttggcg ataacattca catgacctgc aaactgtcta
     1561 ggaaaagaca tcagtttacg tgatctcatc aaccatcagt ttgcgtaatc acatcaacca
     1621 tcaaagcgac agtgatgacg atgcagtgaa gaagggtggg tgtgcagcgc aggaaatcca
     1681 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatatcaa
     1741 gcttatcgat accgtcgacc tcgagggggg gccctgcagc cattgacgac attgcggagc
     1801 catggacgat accccaagcc gaacaagagg gatggacggc attaacgacc ttgatggcca
     1861 tgtctggggt agggaccaaa aggcggtggt ggtattgtca aactaacaaa cctcacccct
     1921 catctcgagc gtggatggaa aaaaggacat tcgaattgaa tctgatgcca agaaatcccg
     1981 ggaatggaag ctctcagagc tctgccacag atagagcttg gagctttggg caagctccca
     2041 gccggcaggg agagctcctc tagacagaaa aggctcaaga agaacatggc aacaaaagta
     2101 aaaatgataa cgtaccggtt gtggaagatg aaatcctgga tgattgtagg atgataatga
     2161 agctaaaagt acaaggtaaa gtccgaacaa acgaacgatg gaaatctgct acacggcgat
     2221 ggatgcacaa caaagacctg agacgagagg gagaacggag tcgggtcggg tgtttaacaa
     2281 tttttactcc atccaaaatg catggaagcg agctccggcg gccgagcgtc ccctcccgcc
     2341 gccccgcgtt gtcggccggc ccggcaatta caagcagaat tcttgcctga aaactcaggc
     2401 ttcaaagagc tgagacgctc gtgccaagct ttcaaagtct tggcagacct cctgggttcc
     2461 actggcttct tggtgtcagg gtgttgggtc aagcgcaggg gtcggccttg gacggcgtca
     2521 gttgaggtca gcgctcagcg gcaccctgac gctaggccac ctcaatgtcc atctgatccc
     2581 gacaagaccg tgaaacgtcg ccctgaaagc gatcagcccg aacaaaaacc accaagtcgc
     2641 cacagcatcc cgcccgtctg gcaatcatca ccgcgaggct cagattggat ctagagaaga
     2701 attaccttag cccatcagaa caattcccag caactccggc gatagacacg tttacgcaat
     2761 ggatttcaat agcctcaagg ataccgtgtc caacctgaca ctctacgaca tcaaggctgg
     2821 cgtacgaaag gttcaaaatg gtaggtgacc acctagtcca gaaccaagac tccctcatga
     2881 tttcgtgttc agggtgctga caatggctcc aaccagctgt catgaactat accgagatgg
     2941 agtccaaggt atgcccgtat ccacgcgcca cactggcacc attctcttgc gcccttcgcc
     3001 atatacttga tcccagacaa gagaccatgg aacgggagct gacagcgaac ccaggtgcgc
     3061 gaggccacca acaatgaacc ttggggtgca tcgtcgactt tgatgcaaga gatcgccgat
     3121 ggcaccttca actagtacgt tctcgagaac tgctcgcttg tatacgaacg cggctaactt
     3181 gcgtttccct ccttgttctt agccaaacac tcaacgaaat catgcccatg atttaccgcc
     3241 gtttcacaga aaagtccgcc gaagaatggc gccagatcta caaggccctt caactactag
     3301 agtacctgat taagcacggt tccgaacgag tggtggacga tgctcggtcg catcttaccc
     3361 tcctcaagat gctgcgccag ttccatttta tcgaccagaa cggcaaagac cagggtatca
     3421 acgtccgcaa ccgcgccaag gaactcgccg agctcctcag cgatgtcgag cgtatccgag
     3481 cagagcgcaa gaaggcccgc gcaaacaagg gcaagttcac gggcatttcg ggtggaatga
     3541 gcttctccag cgggagcagc ggacgttatg gcggtttcgg caacacttcg tatggcggta
     3601 gcagcagtgg tggcggtggt agcagctcta cctacggtgg ctattctgga ggagtttatg
     3661 gcgacggagg tgttgggccc ggtacccagc ttttgttccc tttagtgagg gttaattgcg
     3721 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     3781 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     3841 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     3901 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     3961 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     4021 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     4081 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     4141 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     4201 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4261 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4321 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4381 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4441 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4501 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4561 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4621 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4681 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4741 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4801 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     4861 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     4921 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     4981 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     5041 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     5101 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     5161 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     5221 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     5281 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     5341 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     5401 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     5461 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     5521 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     5581 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     5641 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     5701 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     5761 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     5821 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     5881 gtgccac
//
