LOCUS       40924_13680       11326 bp DNA     circular SYN 13-MAY-2021
DEFINITION  Single copy yeast plasmid expressing Cpf1 from Lachnospiraceae 
            bacterium ND2006 (LbCpf1), codon optimized for expression in 
            Saccharomyces cerevisiae..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11326)
  AUTHORS   Verwaal R, Buiting-Wiessenhaan N, Dalhuijsen S, Roubos JA
  TITLE     CRISPR/Cpf1 enables fast and simple genome editing of Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2017 Sep 8. doi: 10.1002/yea.3278.
  PUBMED    28886218
REFERENCE   2  (bases 1 to 11326)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 11326)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2017
            Sep 8. doi: 10.1002/yea.3278."
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             965..4648
                     /codon_start=1
                     /label=LbCpf1
                     /note="CRISPR-associated protein from Lachnospiraceae
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             4661..4681
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     protein_bind    5030..5063
                     /label=lox66
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            5109..6465
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     protein_bind    6513..6546
                     /label=lox71
                     /note="Left element (LE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(6614..6632)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6653..6669)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6653..6669)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6666..6688)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6677..6693
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6701..6731)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6746..6767)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind     complement(6884..6901)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7055..7643)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7817..8674)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8675..8779)
                     /label=AmpR promoter
     misc_feature    8816..9319
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     primer_bind     9360..9378
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     primer_bind     complement(9416..9438)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9538..9557
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     promoter        9577..9858
                     /label=TRP1 promoter
     CDS             9859..10530
                     /codon_start=1
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(10636..11091)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     11232..11248
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11258..11276
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggcagaggag taggtagagc aacgaatcct actatttatc caaattagtc taggaactct
       61 ttttctagat tttttagatt tgagggcaag cgctgttaac gactcagaaa tgtaagcact
      121 acggagtaga acgagaaatc cgccataggt ggaaatccta gcaaaatctt gcttacccta
      181 gctagcctca ggtaagctag ccttagcctg tcaaattttt ttcaaaattt ggtaagtttc
      241 tactagcaaa gcaaacacgg ttcaacaaac cgaaaactcc actcattata cgtggaaacc
      301 gaaacaaaaa aacaaaaacc aaaatactcg ccaatgagaa agttgctgcg tttctacttt
      361 cgaggaagag gaactgagag gattgactac gaaaggggca aaaacgagtc gtattctccc
      421 attattgtct gctaccacgc ggtctagtag aataagcaac cagtcaacgc taagacaggt
      481 aatcaaaata ccagtctgct ggctacgggc tagtttttac ctcttttaga acccactgta
      541 aaagtccgtt gtaaagcccg ttctcactgt tggcgttttt ttttttttgg tttagtttct
      601 tatttttcat ttttttcttt catgaccaaa aacaaacaaa tctcgcgatt tgtactgcgg
      661 ccactggggc gtggccaaaa aaatgacaaa tttagaaacc ttagtttctg atttttcctg
      721 ttatgaggag atatgataaa aaatattact gctttattgt ttttttttta tctactgaaa
      781 tagagaaact tacccaagga ggaggcaaaa aaaagagtat atatacagca gctaccattc
      841 agattttaat atattctttt ctcttcttct acactattat tataataatt ttactatatt
      901 catttttagc ttaaaacctc atagaatatt attcttcagt cactcgctta aatacttatc
      961 aaaaatgtct aagttggaaa aattcaccaa ctgttactct ttgtctaaga ctttgagatt
     1021 caaggccatc ccagttggta agacccaaga aaacatcgac aacaagagac tattagttga
     1081 agatgaaaag agagctgaag actacaaggg tgtcaagaaa ttgttggaca gatactactt
     1141 gtcttttatc aacgacgttt tgcattccat caagctaaag aacttgaata actacatctc
     1201 tttgttcaga aagaagacta gaactgaaaa ggaaaataag gaattggaaa acttggaaat
     1261 caacttgaga aaggaaattg ctaaggcttt caagggtaat gaaggttaca agtctttatt
     1321 caagaaagac atcattgaaa ccattttgcc agaatttttg gatgataagg atgaaattgc
     1381 tttggttaac tctttcaacg gtttcaccac tgctttcact ggtttcttcg acaacagaga
     1441 aaacatgttc tccgaggaag ctaaatccac ttctattgct ttcagatgta tcaacgaaaa
     1501 cttgacccgt tacatctcta acatggacat ttttgaaaag gtcgacgcca tctttgacaa
     1561 gcacgaagtc caagaaatca aggaaaagat cttaaactcc gactacgatg tcgaagattt
     1621 cttcgaaggt gaattcttca actttgtttt aacccaagaa ggtatcgatg tctacaacgc
     1681 cattatcggt ggttttgtca ctgaatctgg tgaaaagatc aagggtttga acgaatacat
     1741 taacttgtac aaccaaaaga ccaaacaaaa attgccaaag ttcaagccat tgtacaagca
     1801 agttttgtct gacagagaat ctttgtcttt ttacggtgaa gggtacacct ctgacgaaga
     1861 agtcttggaa gtcttcagaa acactttgaa caagaactct gaaatcttct cctccatcaa
     1921 gaagttagaa aagttgttca agaacttcga tgaatactct tctgctggta tcttcgttaa
     1981 gaacggtcca gccatctcta ccatttctaa ggatatcttt ggtgaatgga acgtcattag
     2041 agacaaatgg aacgctgaat acgatgacat ccatttgaag aaaaaggctg ttgtcaccga
     2101 aaagtacgaa gacgacagaa gaaaatcctt caagaagatc ggttccttct ccttggaaca
     2161 attacaagaa tacgccgatg ccgatttgtc cgttgtcgaa aaattgaagg aaattattat
     2221 tcaaaaggtt gatgaaattt acaaagttta cggttcctct gaaaagttat tcgatgctga
     2281 tttcgtcttg gaaaagtctt tgaagaagaa cgacgctgtt gtcgctatca tgaaggactt
     2341 gttggactct gtcaaatctt tcgaaaacta tatcaaggcc ttcttcggtg aaggtaagga
     2401 aactaacaga gatgaatcct tctacggtga ctttgtcttg gcttacgata ttttgttgaa
     2461 ggttgaccac atctacgatg ccatcagaaa ctacgttact caaaagccat actctaagga
     2521 caaattcaag ttgtacttcc aaaacccaca attcatgggt ggttgggata aggacaagga
     2581 aactgactac agagctacca ttttgagata cggttccaag tactacttgg ccatcatgga
     2641 caagaagtac gccaagtgtt tgcaaaagat tgacaaggac gatgtcaacg gtaactacga
     2701 aaagattaac tacaagttgt tgccaggtcc aaacaagatg ttgccaaagg ttttcttctc
     2761 caaaaagtgg atggcttact acaacccatc tgaagacatc caaaagatct acaagaacgg
     2821 tactttcaaa aagggtgaca tgttcaactt aaacgactgt cacaagttga tcgacttctt
     2881 caaggactcc atctctagat acccgaaatg gtccaacgct tacgatttca acttctctga
     2941 aactgaaaaa tacaaggata ttgctggttt ctaccgtgaa gtcgaggaac aaggttataa
     3001 ggtttctttc gaatccgctt ctaagaaaga agttgacaaa ttagtcgaag aaggtaagtt
     3061 gtacatgttc caaatctaca acaaagattt ctccgacaag tctcacggta ctccaaactt
     3121 gcacaccatg tacttcaagt tgctattcga tgaaaacaac cacggtcaaa tcagattgtc
     3181 tggtggtgct gaattgttca tgagacgtgc ttctctaaag aaggaagaat tagtcgtcca
     3241 cccagctaac tctccaattg ccaacaagaa cccagacaac cctaagaaga ccaccacttt
     3301 gtcctacgac gtttacaagg acaagagatt ctccgaagac caatacgaat tgcacattcc
     3361 aattgctatc aacaagtgtc caaagaacat cttcaagatc aacactgaag tcagagtttt
     3421 gttaaagcac gatgacaacc cttacgttat tggtatcgac cgtggtgaaa gaaatttgtt
     3481 gtacattgtt gttgttgacg gtaagggtaa catcgttgaa caatactcct tgaacgaaat
     3541 catcaacaac ttcaacggta ttagaatcaa gactgattac cactctttgt tggataagaa
     3601 ggaaaaggaa cgttttgaag ctcgtcaaaa ctggacctct attgaaaaca tcaaagaatt
     3661 gaaggctggt tacatcagtc aagttgtcca caagatctgt gaattggtcg agaagtacga
     3721 tgccgttatt gccttggaag atttgaactc tggttttaag aactctcgtg tcaaggttga
     3781 aaagcaagtc taccaaaagt tcgaaaagat gttaatcgac aaattgaact acatggttga
     3841 caagaaatcc aacccatgtg ctaccggtgg tgctttgaaa ggttaccaaa tcaccaacaa
     3901 attcgaatct ttcaaatcta tgtccactca aaacgggttc atcttctaca ttccagcttg
     3961 gttgacctcc aagatcgacc catctaccgg tttcgttaac ttgttgaaga ccaagtacac
     4021 ttccattgct gattccaaga agttcatctc ttctttcgac agaatcatgt acgttccaga
     4081 agaagacttg ttcgaattcg ccttggacta taagaacttc tccagaaccg atgctgacta
     4141 cattaagaaa tggaaattgt actcctacgg taacagaatc agaattttca gaaacccaaa
     4201 gaaaaacaac gttttcgatt gggaagaagt ttgtttgact tctgcctaca aggaattatt
     4261 caacaaatac ggtatcaact accaacaagg tgatatcaga gctttgttgt gtgaacaatc
     4321 tgacaaggct ttctactctt ccttcatggc tttgatgtcc ttgatgttgc aaatgagaaa
     4381 ctccatcact ggtagaactg atgtcgactt cctcatttct ccagttaaga attctgacgg
     4441 tattttctac gactctagaa attacgaagc tcaagaaaac gctattttgc caaagaacgc
     4501 tgatgctaac ggtgcttaca atattgctag aaaggttttg tgggctatcg gtcaattcaa
     4561 gaaggctgaa gacgaaaagc tagacaaggt caagattgct atttctaaca aggaatggtt
     4621 ggaatacgct caaacctccg tcaagcactc cagagctgat ccaaagaaga agagaaaggt
     4681 ataaaggagt taaaggcaaa gttttctttt ctagagccgt tcccacaaat aattatacgt
     4741 atatgcttct tttcgtttac tatatatcta tatttacaag cctttattca ctgatgcaat
     4801 ttgtttccaa atactttttt ggagatctca taactagata tcatgatggc gcaacttggc
     4861 gctatcttaa ttactctggc tgccaggccc gtgtagaggg ccgcaagacc ttctgtacgc
     4921 catatagtct ctaagaactt gaacaagttt ctagacctat tgccgccttt cggatcgcta
     4981 ttgttgcggc cgccagctga agcttcgtac gctgcaggtc gacgaattct accgttcgta
     5041 taatgtatgc tatacgaagt tatagatctg tttagcttgc ctcgtccccg ccgggtcacc
     5101 cggccagcga catggaggcc cagaataccc tccttgacag tcttgacgtg cgcagctcag
     5161 gggcatgatg tgactgtcgc ccgtacattt agcccataca tccccatgta taatcatttg
     5221 catccataca ttttgatggc cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcaga
     5281 cctgcgagca gggaaacgct cccctcacag acgcgttgaa ttgtccccac gccgcgcccc
     5341 tgtagagaaa tataaaaggt taggatttgc cactgaggtt cttctttcat atacttcctt
     5401 ttaaaatctt gctaggatac agttctcaca tcacatccga acataaacaa ccatgggtaa
     5461 ggaaaagact cacgtttcga ggccgcgatt aaattccaac atggatgctg atttatatgg
     5521 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg
     5581 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     5641 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     5701 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggcaaaac
     5761 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     5821 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     5881 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     5941 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataagct
     6001 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     6061 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     6121 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     6181 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     6241 gatgctcgat gagtttttct aatcagtact gacaataaaa agattcttgt tttcaagaac
     6301 ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa tgttagcgtg
     6361 atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa
     6421 gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc gattcgatac
     6481 taacgccgcc atccagtgtc gaaaacgagc tcataacttc gtataatgta tgctatacga
     6541 acggtagaat tcgatatcag atccactagt ggcctatgcg gccgccaccg cggtggagct
     6601 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
     6661 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6721 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6781 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6841 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6901 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6961 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7021 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7081 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7141 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7201 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7261 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7321 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7381 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7441 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     7501 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7561 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7621 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7681 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7741 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7801 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7861 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7921 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7981 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8041 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8101 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8161 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8221 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8281 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8341 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8401 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8461 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8521 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8581 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8641 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8701 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8761 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcc ttttcatcac
     8821 gtgctataaa aataattata atttaaattt tttaatataa atatataaat taaaaataga
     8881 aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt ccgaagatgt aaaagactct
     8941 agggggatcg ccaacaaata ctacctttta tcttgctctt cctgctctca ggtattaatg
     9001 ccgaattgtt tcatcttgtc tgtgtagaag accacacacg aaaatcctgt gattttacat
     9061 tttacttatc gttaatcgaa tgtatatcta tttaatctgc ttttcttgtc taataaatat
     9121 atatgtaaag tacgcttttt gttgaaattt tttaaacctt tgtttatttt tttttcttca
     9181 ttccgtaact cttctacctt ctttatttac tttctaaaat ccaaatacaa aacataaaaa
     9241 taaataaaca cagagtaaat tcccaaatta ttccatcatt aaaagatacg aggcgcgtgt
     9301 aagttacagg caagcgatcc gtctaagaaa ccattattat catgacatta acctataaaa
     9361 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     9421 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     9481 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     9541 catcagagca gattgtactg agagtgcacc atagatcacg acattactat atatataata
     9601 taggaagcat ttaatagaac agcatcgtaa tatatgtgta ctttgcagtt atgacgccag
     9661 atggcagtag tggaagatat tctttattga aaaatagctt gtcaccttac gtacaatctt
     9721 gatccggagc ttttcttttt ttgccgatta agaattaatt cggtcgaaaa aagaaaagga
     9781 gagggccaag agggagggca ttggtgacta ttgagcacgt gagtatacgt gattaagcac
     9841 acaaaggcag cttggagtat gtctgttatt aatttcacag gtagttctgg tccattggtg
     9901 aaagtttgcg gcttgcagag cacagaggcc gcagaatgtg ctctagattc cgatgctgac
     9961 ttgctgggta ttatatgtgt gcccaataga aagagaacaa ttgacccggt tattgcaagg
    10021 aaaatttcaa gtcttgtaaa agcatataaa aatagttcag gcactccgaa atacttggtt
    10081 ggcgtgtttc gtaatcaacc taaggaggat gttttggctc tggtcaatga ttacggcatt
    10141 gatatcgtcc aactgcatgg agatgagtcg tggcaagaat accaagagtt cctcggtttg
    10201 ccagttatta aaagactcgt atttccaaaa gactgcaaca tactactcag tgcagcttca
    10261 cagaaacctc attcgtttat tcccttgttt gattcagaag caggtgggac aggtgaactt
    10321 ttggattgga actcgatttc tgactgggtt ggaaggcaag agagccccga aagcttacat
    10381 tttatgttag ctggtggact gacgccagaa aatgttggtg atgcgcttag attaaatggc
    10441 gttattggtg ttgatgtaag cggaggtgtg gagacaaatg gtgtaaaaga ctctaacaaa
    10501 atagcaaatt tcgtcaaaaa tgctaagaaa taggttatta ctgagtagta tttatttaag
    10561 tattgtttgt gcacttgccg atctatgcgg tgtgaaatac cgcacagatg cgtaaggaga
    10621 aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
    10681 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
    10741 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
    10801 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
    10861 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
    10921 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
    10981 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
    11041 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc
    11101 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
    11161 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
    11221 gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga
    11281 attgggtacc ttttcttttt ttgcggtcac ccccatgtgg cgggga
//
