LOCUS       40924_13655        5289 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCS2plus_mVenus-Cdt1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Ozpolat BD, Handberg-Thorsager M, Vervoort M, Balavoine G.
  TITLE     Cell lineage and cell cycling analyses of the 4d micromere using 
            live imaging in the marine annelid Platynereis dumerilii
  JOURNAL   Elife 6 (2017) In press
  PUBMED    29231816
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Ozpolat BD, Handberg-Thorsager M, Vervoort M, Balavoine G.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2017) Marine Biological Laboratory, Eugene Bell 
            Center for Regenerative Biology and Tissue Engineering, 7 Mbl 
            Street, Mrc 212, Woods Hole, MA 02543, USA
REFERENCE   3  (bases 1 to 5289)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5289)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife 6 
            (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-AUG-2017) Marine Biological Laboratory, Eugene Bell Center for 
            Regenerative Biology and Tissue Engineering, 7 Mbl Street, Mrc 212, 
            Woods Hole, MA 02543, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             119..835
                     /label=EYFP
                     /note="enhanced YFP"
     misc_feature    857..1297
                     /note="Region: Cdt1; derived from Platynereis dumerilii"
     polyA_signal    complement(1338..1472)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(1585..1603)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1624..1640)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1648..1664)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1672..1702)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1717..1738)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2026..2614)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2788..3645)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3646..3750)
                     /label=AmpR promoter
     rep_origin      complement(3776..4231)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4305..5289
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattca aggcctctgg atcccaccat
      121 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
      181 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
      241 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
      301 cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc taccccgacc acatgaagca
      361 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
      421 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
      481 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
      541 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
      601 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
      661 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
      721 cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
      781 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtccgg
      841 actcagatct cgtgctatgg ctcaaacttc tgtgacgaat ttctttgctt ctcgcaaacg
      901 tggagccgac atccatgcat caaaaaggag gaaagtgcag agactaaccc aagatgtcga
      961 aacagagaat gttagcgtct ctgagcaggc gaatgcagag ttgcaaagtt cacgacctgc
     1021 tggtaaagga gatttaaagg tccccgaact tcagaagaat gaagtcggtg aagattcgag
     1081 cactgctacc aaaccaagca caagaaaatc ggcacctaga gccacaaaga caagaaaagc
     1141 aagcacttcg acaaggtcaa gccagagaag caagacgaaa gctgcagcca gtgctggtca
     1201 aaaggacata acccaatttc tgtcttcaat tttggaatct gatgacaaaa caaaagatgt
     1261 tttacctgag cctgaaccca cagctgctat tgatgactga taactcgagc tagaactata
     1321 gtgtgttgta ttacgtagat ccagacatga taagatacat tgatgagttt ggacaaacca
     1381 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     1441 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     1501 ttcaggttca gggggaggtg tgggaggttt tttaattcgc ggccgcggcg ccaatgcatt
     1561 gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
     1621 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     1681 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     1741 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     1801 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     1861 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     1921 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1981 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     2041 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2101 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     2161 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2221 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2281 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2341 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2401 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2461 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2521 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2581 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2641 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     2701 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     2761 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     2821 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     2881 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     2941 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     3001 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     3061 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     3121 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     3181 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     3241 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     3301 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     3361 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     3421 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     3481 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     3541 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     3601 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     3661 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     3721 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt
     3781 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     3841 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     3901 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     3961 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     4021 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     4081 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     4141 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     4201 gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt
     4261 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agtcgaccat agccaattca
     4321 atatggcgta tatggactca tgccaattca atatggtgga tctggacctg tgccaattca
     4381 atatggcgta tatggactcg tgccaattca atatggtgga tctggacccc agccaattca
     4441 atatggcgga cttggcacca tgccaattca atatggcgga cttggcactg tgccaactgg
     4501 ggaggggtct acttggcacg gtgccaagtt tgaggagggg tcttggccct gtgccaagtc
     4561 cgccatattg aattggcatg gtgccaataa tggcggccat attggctata tgccaggatc
     4621 aatatatagg caatatccaa tatggcccta tgccaatatg gctattggcc aggttcaata
     4681 ctatgtattg gccctatgcc atatagtatt ccatatatgg gttttcctat tgacgtagat
     4741 agcccctccc aatgggcggt cccatatacc atatatgggg cttcctaata ccgcccatag
     4801 ccactccccc attgacgtca atggtctcta tatatggtct ttcctattga cgtcatatgg
     4861 gcggtcctat tgacgtatat ggcgcctccc ccattgacgt caattacggt aaatggcccg
     4921 cctggctcaa tgcccattga cgtcaatagg accacccacc attgacgtca atgggatggc
     4981 tcattgccca ttcatatccg ttctcacgcc ccctattgac gtcaatgacg gtaaatggcc
     5041 cacttggcag tacatcaata tctattaata gtaacttggc aagtacatta ctattggaag
     5101 gacgccaggg tacattggca gtactcccat tgacgtcaat ggcggtaaat ggcccgcgat
     5161 ggctgccaag tacatcccca ttgacgtcaa tggggagggg caatgacgca aatgggcgtt
     5221 ccattgacgt aaatgggcgg taggcgtgcc taatgggagg tctatataag caatgctcgt
     5281 ttagggaac
//
