LOCUS       40924_13650        4203 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Gateway(R) library vector that allows high-level transient 
            expression in vertebrate cells as well as in vitro 
            transcription/translation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   I.M.A.G.E. Consortium
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     protein_bind    93..117
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(169..193)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(229..247)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    complement(252..386)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(499..517)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(538..554)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(562..578)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(586..616)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(631..652)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(940..1528)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1702..2559)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2560..2664)
                     /label=AmpR promoter
     rep_origin      complement(2690..3145)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3219..4203
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atctctcgaa tcacaagttt gtacaaaaaa gcaggctctc
      121 gagctctaat aaggtgacac tatagaacca ctctccagta ttggatccac ccagctttct
      181 tgtacaaagt ggtgatgatc cgtccgtcga gcctctacat tctgcagccc tatagtgagt
      241 cgtattacgt agatccagac atgataagat acattgatga gtttggacaa accacaacta
      301 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
      361 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
      421 ttcaggggga ggtgtgggag gttttttaat tcgcggccgc ggcgccaatg cattgggccc
      481 ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
      541 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      601 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      661 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
      721 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
      781 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      841 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      901 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
      961 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1021 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     1081 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     1141 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1201 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1261 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1321 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1381 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1441 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1501 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1561 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1621 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1681 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     1741 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     1801 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     1861 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     1921 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     1981 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     2041 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     2101 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     2161 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     2221 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     2281 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     2341 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     2401 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     2461 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     2521 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     2581 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     2641 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc
     2701 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     2761 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     2821 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     2881 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     2941 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     3001 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     3061 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     3121 cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa
     3181 gggcgatcgg tgcgggcctc ttcgctatta cgccagtcga ccatagccaa ttcaatatgg
     3241 cgtatatgga ctcatgccaa ttcaatatgg tggatctgga cctgtgccaa ttcaatatgg
     3301 cgtatatgga ctcgtgccaa ttcaatatgg tggatctgga ccccagccaa ttcaatatgg
     3361 cggacttggc accatgccaa ttcaatatgg cggacttggc actgtgccaa ctggggaggg
     3421 gtctacttgg cacggtgcca agtttgagga ggggtcttgg ccctgtgcca agtccgccat
     3481 attgaattgg catggtgcca ataatggcgg ccatattggc tatatgccag gatcaatata
     3541 taggcaatat ccaatatggc cctatgccaa tatggctatt ggccaggttc aatactatgt
     3601 attggcccta tgccatatag tattccatat atgggttttc ctattgacgt agatagcccc
     3661 tcccaatggg cggtcccata taccatatat ggggcttcct aataccgccc atagccactc
     3721 ccccattgac gtcaatggtc tctatatatg gtctttccta ttgacgtcat atgggcggtc
     3781 ctattgacgt atatggcgcc tcccccattg acgtcaatta cggtaaatgg cccgcctggc
     3841 tcaatgccca ttgacgtcaa taggaccacc caccattgac gtcaatggga tggctcattg
     3901 cccattcata tccgttctca cgccccctat tgacgtcaat gacggtaaat ggcccacttg
     3961 gcagtacatc aatatctatt aatagtaact tggcaagtac attactattg gaaggacgcc
     4021 agggtacatt ggcagtactc ccattgacgt caatggcggt aaatggcccg cgatggctgc
     4081 caagtacatc cccattgacg tcaatgggga ggggcaatga cgcaaatggg cgttccattg
     4141 acgtaaatgg gcggtaggcg tgcctaatgg gaggtctata taagcaatgc tcgtttaggg
     4201 aac
//
