LOCUS       40924_13640        4046 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector that allows high-level transient expression in vertebrate 
            cells as well as in vitro transcription/translation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   I.M.A.G.E. Consortium
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4046
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        34..52
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(103..121)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    complement(128..262)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(381..397)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(405..421)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(429..459)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(474..495)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(783..1371)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1545..2402)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2403..2507)
                     /label=AmpR promoter
     rep_origin      complement(2533..2988)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3062..4046
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag ctttaatacg actcactata gggcaataca
       61 agctacttgt ttaaactctg cagggatccg aattcctcga gccctttagt gagggttaat
      121 ttacgtagat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
      181 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
      241 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
      301 gggggaggtg tgggaggttt tttaattcgc ggccgcggcg ccaatgcatt gggcccggta
      361 ccgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
      421 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
      481 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
      541 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
      601 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
      661 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      721 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      781 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      841 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      901 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      961 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1021 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1081 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1141 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1201 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     1261 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1321 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1381 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1441 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     1501 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     1561 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1621 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1681 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1741 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1801 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1861 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1921 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1981 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     2041 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     2101 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     2161 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     2221 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     2281 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     2341 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     2401 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     2461 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     2521 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     2581 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     2641 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     2701 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     2761 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     2821 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     2881 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     2941 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat
     3001 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagt
     3061 cgaccatagc caattcaata tggcgtatat ggactcatgc caattcaata tggtggatct
     3121 ggacctgtgc caattcaata tggcgtatat ggactcgtgc caattcaata tggtggatct
     3181 ggaccccagc caattcaata tggcggactt ggcaccatgc caattcaata tggcggactt
     3241 ggcactgtgc caactgggga ggggtctact tggcacggtg ccaagtttga ggaggggtct
     3301 tggccctgtg ccaagtccgc catattgaat tggcatggtg ccaataatgg cggccatatt
     3361 ggctatatgc caggatcaat atataggcaa tatccaatat ggccctatgc caatatggct
     3421 attggccagg ttcaatacta tgtattggcc ctatgccata tagtattcca tatatgggtt
     3481 ttcctattga cgtagatagc ccctcccaat gggcggtccc atataccata tatggggctt
     3541 cctaataccg cccatagcca ctcccccatt gacgtcaatg gtctctatat atggtctttc
     3601 ctattgacgt catatgggcg gtcctattga cgtatatggc gcctccccca ttgacgtcaa
     3661 ttacggtaaa tggcccgcct ggctcaatgc ccattgacgt caataggacc acccaccatt
     3721 gacgtcaatg ggatggctca ttgcccattc atatccgttc tcacgccccc tattgacgtc
     3781 aatgacggta aatggcccac ttggcagtac atcaatatct attaatagta acttggcaag
     3841 tacattacta ttggaaggac gccagggtac attggcagta ctcccattga cgtcaatggc
     3901 ggtaaatggc ccgcgatggc tgccaagtac atccccattg acgtcaatgg ggaggggcaa
     3961 tgacgcaaat gggcgttcca ttgacgtaaa tgggcggtag gcgtgcctaa tgggaggtct
     4021 atataagcaa tgctcgttta gggaac
//
