LOCUS       40924_13620        5753 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCS2-sfGFP-DrAqp3a_tVE1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Eskova A, Garcia-Junco R, Chauvigne F, Maischein H-M., Ammelburg M, 
            Cerda J, Kaderali L, Nusslein-Volhard C, Irion U.
  TITLE     Gain-of-function mutations of mau/DrAqp3a influence zebrafish 
            pigment pattern formation through the tissue environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   Eskova A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2016) e-CNV group, Max Planck Institute for 
            Developmental Biology, Spemannstr. 35-39, Tuebingen 72076, Germany
REFERENCE   3  (bases 1 to 5753)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5753)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-JAN-2016) e-CNV group, Max Planck Institute for Developmental 
            Biology, Spemannstr. 35-39, Tuebingen 72076, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             107..823
                     /codon_start=1
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(1780..1797)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    complement(1802..1936)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(2049..2067)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2088..2104)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2112..2128)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2136..2166)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2181..2202)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2490..3078)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3252..4109)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4110..4214)
                     /label=AmpR promoter
     rep_origin      complement(4240..4695)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4769..5753
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaatagc gccaccatgg tgtctaaagg
      121 agaggagctg ttcacaggcg tggtgccaat cctggtggag ctggatggag acgtgaacgg
      181 ccacaagttc agcgtgagag gcgagggaga gggagacgcc acaaacggca agctgacact
      241 gaaattcatc tgcacaacag gcaaactgcc tgtgccttgg ccaaccctgg tgacaaccct
      301 gacatacgga gtgcagtgct ttagcagata ccctgatcac atgaaacagc acgatttctt
      361 caagagcgcc atgcctgagg gctacgtgca ggagagaacc atcagcttca aggatgacgg
      421 aacctacaag acaagagccg aggtgaagtt tgagggagat acactggtga acagaatcga
      481 gctgaaaggc atcgatttca aagaggatgg caacatcctg ggacacaaac tggagtacaa
      541 cttcaacagc cacaacgtgt acatcacagc cgataaacag aagaacggca tcaaagccaa
      601 cttcaagatc agacacaatg tggaggatgg atctgtgcag ctggccgatc actaccagca
      661 gaacacacct atcggagacg gcccagtgct gctgccagat aaccactacc tgagcacaca
      721 gagcgtgctg tctaaagacc ctaacgagaa gagagatcac atggtgctgc tggagtttgt
      781 gacagccgcc ggaatcaccc tgggcatgga tgagctgtac aaatccggac tcagatctat
      841 gggttggcag aaaagcgttc tggataagct tgcgcagact ttccaaatcc gcaacaagtt
      901 actgcgccag ggattggctg aatgcttagg aactctcatc ctggtgatgt ttggctgtgg
      961 ttcattggcc cagttgaaac taagcgaagg ttctcatggt ctctttctca ctgcaaacct
     1021 tgcttttggg tttggtgcta ctcttggaat cttggtttgc ggccaggtgt caggcggaca
     1081 tttaaatcct gctgttacat ttgctctctg cctcttggga agagaaaaat ggagaaagtt
     1141 tcctgtgtac tttctgttcc aaacactcgg atccttcttg ggtgctgcta ttatctttgc
     1201 cgaataccat gacgcaattt atgattatgc tggagaatca aatgagttgc ttgtactggg
     1261 tgaaaaagaa acagctggga tttttgctac atacccaagc aaatatctca cccccctaaa
     1321 tggatttttt gaccaggtga taggcacagc atccctgatt gtgtgcatcc tggccattgt
     1381 ggacccctac aacaacccga tccctcaagg tcttgaggcc ttcacagtgg gattcagcgt
     1441 ccttatcatt ggtctctcca tgggcttcaa ttctggctat gcagtaaacc cagctcaaga
     1501 ttttggacct cgtcttttta ctgccatggc tggttggggt agtgaagtct tcacagccag
     1561 ggattattgg tttttggtgc ccatctttgc tccgttcatt ggagccgtta ttggtgtgat
     1621 tgtgtaccag ctgatggtgg gatggcatgt ggaaggagag gcacgagata agaaagctaa
     1681 agctagagag gaggtgatga acctcaatga cgtcgccagc aaggaatgag aattctgcag
     1741 tcgacggtac cgcgggcccg ggatccaccg gatctagaac tatagtgagt cgtattacgt
     1801 agatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     1861 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     1921 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     1981 ggtgtgggag gttttttaat tcgcggccgc ggcgccaatg cattgggccc ggtacccagc
     2041 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
     2101 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     2161 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     2221 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     2281 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     2341 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     2401 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     2461 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2521 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2581 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2641 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     2701 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2761 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2821 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2881 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     2941 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     3001 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     3061 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     3121 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     3181 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     3241 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     3301 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     3361 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     3421 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3481 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3541 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     3601 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     3661 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3721 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3781 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3841 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3901 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3961 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     4021 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     4081 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     4141 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     4201 ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt
     4261 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     4321 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     4381 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     4441 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     4501 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     4561 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     4621 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     4681 cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     4741 tgcgggcctc ttcgctatta cgccagtcga ccatagccaa ttcaatatgg cgtatatgga
     4801 ctcatgccaa ttcaatatgg tggatctgga cctgtgccaa ttcaatatgg cgtatatgga
     4861 ctcgtgccaa ttcaatatgg tggatctgga ccccagccaa ttcaatatgg cggacttggc
     4921 accatgccaa ttcaatatgg cggacttggc actgtgccaa ctggggaggg gtctacttgg
     4981 cacggtgcca agtttgagga ggggtcttgg ccctgtgcca agtccgccat attgaattgg
     5041 catggtgcca ataatggcgg ccatattggc tatatgccag gatcaatata taggcaatat
     5101 ccaatatggc cctatgccaa tatggctatt ggccaggttc aatactatgt attggcccta
     5161 tgccatatag tattccatat atgggttttc ctattgacgt agatagcccc tcccaatggg
     5221 cggtcccata taccatatat ggggcttcct aataccgccc atagccactc ccccattgac
     5281 gtcaatggtc tctatatatg gtctttccta ttgacgtcat atgggcggtc ctattgacgt
     5341 atatggcgcc tcccccattg acgtcaatta cggtaaatgg cccgcctggc tcaatgccca
     5401 ttgacgtcaa taggaccacc caccattgac gtcaatggga tggctcattg cccattcata
     5461 tccgttctca cgccccctat tgacgtcaat gacggtaaat ggcccacttg gcagtacatc
     5521 aatatctatt aatagtaact tggcaagtac attactattg gaaggacgcc agggtacatt
     5581 ggcagtactc ccattgacgt caatggcggt aaatggcccg cgatggctgc caagtacatc
     5641 cccattgacg tcaatgggga ggggcaatga cgcaaatggg cgttccattg acgtaaatgg
     5701 gcggtaggcg tgcctaatgg gaggtctata taagcaatgc tcgtttaggg aac
//
