LOCUS       V015191                 5462 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015191
VERSION     V015191
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        52..1036
                     /label="CMV IE94 promoter"
                     /note="enhancer/promoter region of simian cytomegalovirus
                     major immediate early transcription unit IE94"
     promoter        1071..1089
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             1136..2479
                     /gene="FBLN5"
                     /label="Fibulin-5"
                     /note="Fibulin-5 from Homo sapiens. Accession#: Q9UBX5"
     CDS             2489..2506
                     /label="6xHis"
                     /note="6xHis affinity tag"
     polyA_signal    complement(2547..2681)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(2794..2812)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2833..2849)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2857..2873)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2881..2911)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2926..2947)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3235..3823)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3997..4854)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4855..4959)
                     /label="AmpR promoter"
     rep_origin      complement(4985..5440)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagt cgaccatagc
       61 caattcaata tggcgtatat ggactcatgc caattcaata tggtggatct ggacctgtgc
      121 caattcaata tggcgtatat ggactcgtgc caattcaata tggtggatct ggaccccagc
      181 caattcaata tggcggactt ggcaccatgc caattcaata tggcggactt ggcactgtgc
      241 caactgggga ggggtctact tggcacggtg ccaagtttga ggaggggtct tggccctgtg
      301 ccaagtccgc catattgaat tggcatggtg ccaataatgg cggccatatt ggctatatgc
      361 caggatcaat atataggcaa tatccaatat ggccctatgc caatatggct attggccagg
      421 ttcaatacta tgtattggcc ctatgccata tagtattcca tatatgggtt ttcctattga
      481 cgtagatagc ccctcccaat gggcggtccc atataccata tatggggctt cctaataccg
      541 cccatagcca ctcccccatt gacgtcaatg gtctctatat atggtctttc ctattgacgt
      601 catatgggcg gtcctattga cgtatatggc gcctccccca ttgacgtcaa ttacggtaaa
      661 tggcccgcct ggctcaatgc ccattgacgt caataggacc acccaccatt gacgtcaatg
      721 ggatggctca ttgcccattc atatccgttc tcacgccccc tattgacgtc aatgacggta
      781 aatggcccac ttggcagtac atcaatatct attaatagta acttggcaag tacattacta
      841 ttggaaggac gccagggtac attggcagta ctcccattga cgtcaatggc ggtaaatggc
      901 ccgcgatggc tgccaagtac atccccattg acgtcaatgg ggaggggcaa tgacgcaaat
      961 gggcgttcca ttgacgtaaa tgggcggtag gcgtgcctaa tgggaggtct atataagcaa
     1021 tgctcgttta gggaaccgcc attctgcctg gggacgtcgg agcaagcttg atttaggtga
     1081 cactatagaa tacaagctac ttgttctttt tgcaggatcc catcgattcg aattcatgcc
     1141 aggaataaaa aggatactca ctgttaccat tctggctctc tgtcttccaa gccctgggaa
     1201 tgcacaggca cagtgcacga atggctttga cctggatcgc cagtcaggac agtgtttaga
     1261 tattgatgaa tgccgaacca tccccgaggc ctgccgagga gacatgatgt gtgttaacca
     1321 aaatggcggg tatttatgca ttccccggac aaaccctgtg tatcgagggc cctactcgaa
     1381 cccctactcg accccctact caggtccgta cccagcagct gccccaccac tctcagctcc
     1441 aaactatccc acgatctcca ggcctcttat atgccgcttt ggataccaga tggatgaaag
     1501 caaccaatgt gtggatgtgg acgagtgtgc aacagattcc caccagtgca accccaccca
     1561 gatctgcatc aatactgaag gcgggtacac ctgctcctgc accgacggat attggcttct
     1621 ggaaggccag tgcttagaca ttgatgaatg tcgctatggt tactgccagc agctctgtgc
     1681 gaatgttcct ggatcctatt cttgtacatg caaccctggt tttaccctca atgaggatgg
     1741 aaggtcttgc caagatgtga acgagtgtgc caccgagaac ccctgcgtgc aaacctgcgt
     1801 caacacctac ggctctttca tctgccgctg tgacccagga tatgaacttg aggaagatgg
     1861 cgttcattgc agtgatatgg acgagtgcag cttctctgag ttcctctgcc aacatgagtg
     1921 tgtgaaccag cccggcacat acttctgctc ctgccctcca ggctacatcc tgctggatga
     1981 caaccgaagc tgccaagaca tcaacgaatg tgagcacagg aaccacacgt gcaacctgca
     2041 gcagacgtgc tacaatttac aagggggctt caaatgcatt gaccccatcc gctgtgagga
     2101 gccttatctg aggatcagtg ataaccgctg tatgtgtcct gctgagaacc ctggctgcag
     2161 agaccagccc tttaccatct tgtaccggga catggacgtg gtgtcaggac gctccgttcc
     2221 cgctgacatc ttccaaatgc aagccacgac ccgctaccct ggggcctatt acattttcca
     2281 gatcaaatct gggaatgagg gcagagaatt ttacatgcgg caaacgggcc ccatcagtgc
     2341 caccctggtg atgacacgcc ccatcaaagg gccccgggaa atccagctgg acttggaaat
     2401 gatcactgtc aacactgtca tcaacttcag aggcagctcc gtgatccgac tgcggatata
     2461 tgtgtcgcag tacccattcg gcggaagtca tcatcatcat catcactgac tcgagcctct
     2521 agaactatag tgagtcgtat tacgtagatc cagacatgat aagatacatt gatgagtttg
     2581 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     2641 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     2701 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaattcgcg gccgcggcgc
     2761 caatgcattg ggcccggtac ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
     2821 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     2881 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     2941 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     3001 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     3061 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     3121 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3181 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     3241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     3301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     3361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     3421 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     3481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     3541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     3601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     3661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     3721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     3781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     3901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     3961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     4021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     4081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     4141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     4201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     4261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     4321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     4381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     4441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     4501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     4561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     4621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     4681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     4741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     4801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     4861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     4921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     4981 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     5041 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     5101 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     5161 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     5221 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     5281 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     5341 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     5401 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg
     5461 cg
//
