LOCUS       V008152                 8438 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008152
VERSION     V008152
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8438)
  AUTHORS   Cerrizuela S, Vega-Lopez GA, Palacio MB, Aybar MJ.
  TITLE     Gli2 is required for the induction and migration of Xenopus laevis
            neural crest
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8438)
  AUTHORS   Cerrizuela S, Vega-Lopez GA, Palacio MB, Aybar MJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2017) Developmental Biology, Instituto Superior de
            Investigaciones Biologicas (INSIBIO, CONICET), Chacabuco 461, San
            Miguel de Tucuman, Tucuman T4000ILI, Argentina
REFERENCE   3  (bases 1 to 8438)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8438)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-JUL-2017) Developmental Biology, Instituto Superior de
            Investigaciones Biologicas (INSIBIO, CONICET), Chacabuco 461, San
            Miguel de Tucuman, Tucuman T4000ILI, Argentina"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             10..4092
                     /gene="gli4"
                     /label="Zinc finger protein GLI4"
                     /note="Zinc finger protein GLI4 from Xenopus laevis.
                     Accession#: Q91661"
     CDS             4111..4140
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     CDS             4150..4179
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     CDS             4189..4218
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     CDS             4228..4257
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     CDS             4267..4296
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     CDS             4330..4359
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     promoter        complement(4387..4404)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      complement(4388..4404)
                     /regulatory_class="promoter"
     polyA_signal    complement(4409..4543)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(4656..4674)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4695..4711)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4719..4735)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4743..4773)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4788..4809)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5097..5685)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5859..6716)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6717..6821)
                     /label="AmpR promoter"
     rep_origin      complement(6847..7302)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7376..8360
                     /label="CMV IE94 promoter"
                     /note="enhancer/promoter region of simian cytomegalovirus
                     major immediate early transcription unit IE94"
     promoter        8395..8413
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 ggatcccaga tggaggatta cgtgggttgt gtgcacaaca gccccaccct gtctatgatc
       61 tctgcagccc gtggtctcag cccagcagaa gttgcccatg agcacttaaa agaaaggggt
      121 atctatggtt tagcacctcc gccgccccct ggtaccaccc ctacagaata ctgccaccaa
      181 atggccttcc tggcaagtca tgcaagtcca tacggggagc tcctggtgca gagcgcagca
      241 gctggaaata cgtcgcacct tcacgattac ctcaccccca tggatgtatc aagattctcc
      301 agcccccgag ttacaccgag attaagccgg aaacgggccc tttccatctc tcctctgtcc
      361 gatgccagca ttgacctgca gaccatgatc cgcacctccc ccaactcgct ggttgcttac
      421 atcaacaact ctcggagcag ctcagccgcc agcgggtcct acgggcacct ttctgcagga
      481 gcaataagcc cggcgtttag cttccccatc cataaaccct gtagcctatc agcagctctg
      541 agccagcagc gtagcctgag ttcctcattt ggacacaccc ctttgctcca cccatcgccg
      601 acgtttgcat cccgccagca gggagcactc acatcagcca atccagctcc tcccagcaac
      661 aacagctccg cccctgactc cgtcctgaac aaggtgagca gtgagtctgc ggtgagcagt
      721 acagtcaatc aggtgataca caaacgcagc aaagtgaaga ctgaagaaga ggccgattca
      781 gtgcgattcc cccagcctcc ggaccacctg actgatctga aagaggattt ggataaggat
      841 gagtgcaagc aggagcctga acatatatac gagaccaact gccactggga cggctgcagc
      901 aaggagtttg atacccagga tcagctcgta catcacatca ataatgacca tatccatggg
      961 gagaagaagg agtttgtgtg tcgctggcag gactgctccc gggagcagaa accattcaag
     1021 gcgcagtata tgctggtggt tcacatgaga aggcacacgg gggagaaacc acataaatgc
     1081 acctttgaag gttgtttcaa ggcctattcc aggctggaga acctgaagac gcacttgcgt
     1141 tcacacaccg gggagaagcc atatgtctgt gaccacgagg gatgcaataa ggcgttttcc
     1201 aatgcgtctg atcgagccaa acatcagaac agaacccatt ccaatgagaa accgtacata
     1261 tgtaaggtcc caggctgcac aaaacgttac acggacccca gctctctcag gaaacacgtt
     1321 aaaactgtac atggacctga ggcccatgtg accaagaagc accgcaatga catcatccaa
     1381 aaaccttcac tacccaagga gaacggggac aatgaagcta gtgccaaact cagcggtagg
     1441 gagcactccg acagtgtctc tagagaccag gagcactgct tgcagaccag aaccatcaaa
     1501 acagaagata acatgatgca tcagtccagc cccggtgggc agtcgtcatg cagcagtgag
     1561 ccttccccat atggcaacac caacaacatt gacagtggag tagatgtgag ccttgcatgg
     1621 caggggagct tgggagacct gtttggcttg gaggagacca gcccggtggt agactccaca
     1681 gtatcatcct ggcagcgcag tggtcggcct gcaactccgg aaacacagcg tatccacagt
     1741 gcagagactg gaacagctga agaaagagaa attaaagaca atgaaagatt cctgctcatt
     1801 tatgaaccca atgccacctg ccagaacacc cggctcccga ccatcagtgc aaatggtttc
     1861 gatgtaatag gtgtccccag cagtgtgcta atcaacccca gagcaataga actgtccatg
     1921 aatgatgtga ccatgatgaa ccaattgaat gagcgccgtg acagcaccag cagcaccctc
     1981 agctcagcat acaccagccg caggtcctcc ggcatctctc cttacttctc cagtcgccgc
     2041 tcaagtgaaa cctcacagtt tggaggccgc ttgaataatt ccagctctgc agattcttac
     2101 gatcccattt ccaccgatgc ctctaggcgc tctagtgagg ccagccagca cagtggactt
     2161 cctaatcttc tcaacctcac tcctgctcag cattacagat taaaggccaa gtatgctgca
     2221 gcaactggtg gtccaccacc cactcctctt cccaacatgg ataggatagg tttgagaaac
     2281 aagctgtccc tgatggatgg agcagacttt cctttgcctc ccttccggca acttcctgtt
     2341 ccaagacgtt gtagtgatgg tggtggaaat gcaggactaa cccccatgta tccccatgaa
     2401 attccgggga ataattcaag gcgggctagt gacccagtca gaagaactgc aggtattgat
     2461 gacaagccac tcccaaggtt cagccgcttt catagcatga atagtatgaa tacattgcac
     2521 cctccatccc tatcggaaag gagaaatgga ggcctgcagc actacacctg ctctgatgga
     2581 ggccttcaca ggcacgtcta ttctccccga ccacccagca ttagtgaaaa tgttgctatg
     2641 gaggccatat cttgtgatgc agatgtccct ggtggagatg atgacctcat gctcccagat
     2701 gatgttgttc agtacatcag atctcagaac agagaagccc cagagcaaaa cctacagacg
     2761 gagtacagca gtccagcccg gaatctacaa agcaacacaa aatctttcca taacaataca
     2821 ccagaacaac ccagggcccc tggggcttac ttgagcagga actttcctgc tttggctgaa
     2881 tgccttggcc agactgctaa catgcaggat aacaacatgc ccgttcagtg gaacgaagtc
     2941 agttcgggta ccgttgatgt cagtgactta cccaagcagc agtttgcagc aggcaactta
     3001 gcagttgttc agcaaaaaca gaactttgcc cagtatcaga gcttcaacca agcacctatg
     3061 cagagggctc acaatattat gggtcagggg caagagtctg tacagaggaa tatcagtgtt
     3121 aatggacagc ggtttaatta tttgcaacaa agacaacagc agatgagcca gtgccaaatt
     3181 gtcagttctg acttcatccc ccaacagagg tacagccaga gccagtcaat gctgagttca
     3241 agagcaatgc aagaaggcca gagccagatt tctccatcct gtaataacat ggtagagagg
     3301 cctggggttc acacacatgc tgctccaagc aacacactga atcatcagcg ccttgctgtg
     3361 cacggagcac ccacgcaggg ttttgcaaat aacttctccg ttaaccaaga cgggctccac
     3421 ccacccaacg cctacacagt ccagcctcag aaaaatggat tggagccaca acagaacact
     3481 cttggaatgt ctgggcaagc gttcaaccat ggaatgattc agccaagacc cccagctgcc
     3541 ccacaccccc ctaacagacc caggaatata cacccggtac atcaccctcc ttacatgagg
     3601 agtccgcacc ctgtgagtga actgagccca ggccaacaaa cagcagaggc aacaccaaaa
     3661 cgaaccagtg aaaacactga tccaactcct aaagacaata atttgcttta ttattctgga
     3721 caaattcaca tgtacgagcc aaatggaaac ttcggttctg gcatagactg cacagtacgg
     3781 caactaccca ccatgccgtc tcctggggca aatcaggtca caagcactgt agactcccaa
     3841 ggtcttgagc acccccctgt tatagatttt gacgccatga tggatgatgg ggatcactcg
     3901 agccttatgt caggaacctt gagccctggc ctcctgcaga gcttctccca aacatcctcc
     3961 cgccttacaa ctcccaggaa ttccctgacg ctcccttcca tccctgctgg gataaacaac
     4021 atggcaatag gagacatgag ctccatgctc acaacattgg cggaggaaag caagtttctc
     4081 aatctgatgt cctatcgatt taaagctatg gagcaaaagc tcatttctga agaggacttg
     4141 aatgaaatgg agcaaaagct catttctgaa gaggacttga atgaaatgga gcaaaagctc
     4201 atttctgaag aggacttgaa tgaaatggag caaaagctca tttctgaaga ggacttgaat
     4261 gaaatggagc aaaagctcat ttctgaagag gacttgaatg aaatggagag cttgggcgac
     4321 ctcaccatgg agcaaaagct catttctgaa gaggacttga attcaaggcc tctcgagcct
     4381 ctagaactat agtgagtcgt attacgtaga tccagacatg ataagataca ttgatgagtt
     4441 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     4501 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     4561 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaattcg cggccgcggc
     4621 gccaatgcat tgggcccggt acccagcttt tgttcccttt agtgagggtt aattgcgcgc
     4681 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     4741 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     4801 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     4861 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     4921 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     4981 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     5041 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     5101 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     5161 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     5221 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     5281 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     5341 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     5401 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     5461 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     5521 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     5581 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     5641 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     5701 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     5761 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     5821 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     5881 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     5941 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     6001 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     6061 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     6121 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     6181 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     6241 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     6301 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     6361 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     6421 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     6481 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     6541 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     6601 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     6661 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     6721 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     6781 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     6841 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     6901 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     6961 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     7021 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     7081 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     7141 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     7201 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     7261 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
     7321 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagtcgacca
     7381 tagccaattc aatatggcgt atatggactc atgccaattc aatatggtgg atctggacct
     7441 gtgccaattc aatatggcgt atatggactc gtgccaattc aatatggtgg atctggaccc
     7501 cagccaattc aatatggcgg acttggcacc atgccaattc aatatggcgg acttggcact
     7561 gtgccaactg gggaggggtc tacttggcac ggtgccaagt ttgaggaggg gtcttggccc
     7621 tgtgccaagt ccgccatatt gaattggcat ggtgccaata atggcggcca tattggctat
     7681 atgccaggat caatatatag gcaatatcca atatggccct atgccaatat ggctattggc
     7741 caggttcaat actatgtatt ggccctatgc catatagtat tccatatatg ggttttccta
     7801 ttgacgtaga tagcccctcc caatgggcgg tcccatatac catatatggg gcttcctaat
     7861 accgcccata gccactcccc cattgacgtc aatggtctct atatatggtc tttcctattg
     7921 acgtcatatg ggcggtccta ttgacgtata tggcgcctcc cccattgacg tcaattacgg
     7981 taaatggccc gcctggctca atgcccattg acgtcaatag gaccacccac cattgacgtc
     8041 aatgggatgg ctcattgccc attcatatcc gttctcacgc cccctattga cgtcaatgac
     8101 ggtaaatggc ccacttggca gtacatcaat atctattaat agtaacttgg caagtacatt
     8161 actattggaa ggacgccagg gtacattggc agtactccca ttgacgtcaa tggcggtaaa
     8221 tggcccgcga tggctgccaa gtacatcccc attgacgtca atggggaggg gcaatgacgc
     8281 aaatgggcgt tccattgacg taaatgggcg gtaggcgtgc ctaatgggag gtctatataa
     8341 gcaatgctcg tttagggaac cgccattctg cctggggacg tcggagcaag cttgatttag
     8401 gtgacactat agaatacaag ctacttgttc tttttgca
//
