LOCUS       40924_13530        4149 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector that allows high-level transient expression in vertebrate 
            cells as well as in vitro transcription/translation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4149)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        complement(167..185)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    complement(190..324)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(441..459)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(480..496)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(504..520)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(528..558)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(573..594)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(882..1470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1644..2501)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2502..2606)
                     /label=AmpR promoter
     rep_origin      complement(2632..3087)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3165..4149
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattcg tcgacaggcc aatctggccg
      121 cggccgcaag gcctgatatc tctagaccgc ggctcgagcc tctcgcccta tagtgagtcg
      181 tattacgtag atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
      241 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
      301 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
      361 cagggggagg tgtgggaggt tttttaattc gcggcgcgcc gcggcgccaa tgcattgggc
      421 ccggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
      481 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
      541 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
      601 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
      661 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
      721 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
      781 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      841 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
      901 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      961 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1021 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1081 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1141 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1201 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1261 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1321 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1381 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1441 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1501 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     1561 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     1621 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     1681 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     1741 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     1801 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     1861 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     1921 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     1981 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     2041 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     2101 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     2161 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     2221 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     2281 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     2341 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     2401 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     2461 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     2521 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     2581 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
     2641 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2701 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2761 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2821 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2881 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2941 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     3001 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     3061 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
     3121 aagggcgatc ggtgcgggcc tcttcgctat tacgccagtc gatcgaccat agccaattca
     3181 atatggcgta tatggactca tgccaattca atatggtgga tctggacctg tgccaattca
     3241 atatggcgta tatggactcg tgccaattca atatggtgga tctggacccc agccaattca
     3301 atatggcgga cttggcacca tgccaattca atatggcgga cttggcactg tgccaactgg
     3361 ggaggggtct acttggcacg gtgccaagtt tgaggagggg tcttggccct gtgccaagtc
     3421 cgccatattg aattggcatg gtgccaataa tggcggccat attggctata tgccaggatc
     3481 aatatatagg caatatccaa tatggcccta tgccaatatg gctattggcc aggttcaata
     3541 ctatgtattg gccctatgcc atatagtatt ccatatatgg gttttcctat tgacgtagat
     3601 agcccctccc aatgggcggt cccatatacc atatatgggg cttcctaata ccgcccatag
     3661 ccactccccc attgacgtca atggtctcta tatatggtct ttcctattga cgtcatatgg
     3721 gcggtcctat tgacgtatat ggcgcctccc ccattgacgt caattacggt aaatggcccg
     3781 cctggctcaa tgcccattga cgtcaatagg accacccacc attgacgtca atgggatggc
     3841 tcattgccca ttcatatccg ttctcacgcc ccctattgac gtcaatgacg gtaaatggcc
     3901 cacttggcag tacatcaata tctattaata gtaacttggc aagtacatta ctattggaag
     3961 tacgccaggg tacattggca gtactcccat tgacgtcaat ggcggtaaat ggcccgcgat
     4021 ggctgccaag tacatcccca ttgacgtcaa tggggagggg caatgacgca aatgggcgtt
     4081 ccattgacgt aaatgggcgg taggcgtgcc taatgggagg tctatataag caatgctcgt
     4141 ttagggaac
//
