LOCUS       40924_13525        4131 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector that allows high-level transient expression in vertebrate 
            cells as well as in vitro transcription/translation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4131)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        complement(149..167)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    complement(172..306)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(423..441)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(462..478)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(486..502)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(510..540)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(555..576)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(864..1452)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1626..2483)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2484..2588)
                     /label=AmpR promoter
     rep_origin      complement(2614..3069)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3147..4131
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattcg tcgacaggcc aatctggccg
      121 cggccgcaag gcctctcgag cctctcgccc tatagtgagt cgtattacgt agatccagac
      181 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
      241 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
      301 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
      361 gttttttaat tcgcggcgcg ccgcggcgcc aatgcattgg gcccggtacc cagcttttgt
      421 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
      481 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      541 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
      601 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      661 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
      721 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      781 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      841 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      901 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      961 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1021 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1081 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1141 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1201 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1261 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     1321 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1381 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1441 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1501 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1561 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1621 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1681 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1741 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     1801 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1861 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1921 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     1981 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     2041 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     2101 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     2161 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     2221 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     2281 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     2341 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     2401 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     2461 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     2521 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     2581 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
     2641 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     2701 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     2761 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     2821 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     2881 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     2941 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     3001 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     3061 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     3121 cctcttcgct attacgccag tcgatcgacc atagccaatt caatatggcg tatatggact
     3181 catgccaatt caatatggtg gatctggacc tgtgccaatt caatatggcg tatatggact
     3241 cgtgccaatt caatatggtg gatctggacc ccagccaatt caatatggcg gacttggcac
     3301 catgccaatt caatatggcg gacttggcac tgtgccaact ggggaggggt ctacttggca
     3361 cggtgccaag tttgaggagg ggtcttggcc ctgtgccaag tccgccatat tgaattggca
     3421 tggtgccaat aatggcggcc atattggcta tatgccagga tcaatatata ggcaatatcc
     3481 aatatggccc tatgccaata tggctattgg ccaggttcaa tactatgtat tggccctatg
     3541 ccatatagta ttccatatat gggttttcct attgacgtag atagcccctc ccaatgggcg
     3601 gtcccatata ccatatatgg ggcttcctaa taccgcccat agccactccc ccattgacgt
     3661 caatggtctc tatatatggt ctttcctatt gacgtcatat gggcggtcct attgacgtat
     3721 atggcgcctc ccccattgac gtcaattacg gtaaatggcc cgcctggctc aatgcccatt
     3781 gacgtcaata ggaccaccca ccattgacgt caatgggatg gctcattgcc cattcatatc
     3841 cgttctcacg ccccctattg acgtcaatga cggtaaatgg cccacttggc agtacatcaa
     3901 tatctattaa tagtaacttg gcaagtacat tactattgga agtacgccag ggtacattgg
     3961 cagtactccc attgacgtca atggcggtaa atggcccgcg atggctgcca agtacatccc
     4021 cattgacgtc aatggggagg ggcaatgacg caaatgggcg ttccattgac gtaaatgggc
     4081 ggtaggcgtg cctaatggga ggtctatata agcaatgctc gtttagggaa c
//
