LOCUS       62056_8000        4387 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..997
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus
                     major immediate early transcription unit IE94"
     promoter        1032..1050
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    complement(1436..1570)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(1683..1701)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1722..1738)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1746..1762)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1770..1800)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1815..1836)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2124..2712)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2886..3743)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3744..3848)
                     /label=AmpR promoter
     rep_origin      complement(3874..4329)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gctattacgc cagtcgaccg ccaattcaat atggcgtata tggactcatg ccaattcaat
       61 atggtggatc tggacctgtg ccaattcaat atggcgtata tggactcgtg ccaattcaat
      121 atggtggatc tggaccccag ccaattcaat atggcggact tggcaccatg ccaattcaat
      181 atggcggacc tggcactgtg ccaactgggg aggggtctac ttggcacggt gccaagtttg
      241 aggaggggtc ttggccctgt gccaagtccg ccatattgaa ttggcatggt gccaataatg
      301 gcggccatat tggctatatg ccaggatcaa tatataggca atatccaata tggccctatg
      361 ccaatatggc tattggccag gttcaatact atgtattggc cctatgccat atagtattcc
      421 atatatgggt tttcctattg acgtagatag cccctcccaa tgggcggtcc catataccat
      481 atatggggct tcctaatacc gcccatagcc actcccccat tgacgtcaat ggtctctata
      541 tatggtcttt cctattgacg tcatatgggc ggtcctattg acgtatatgg cgcctccccc
      601 attgacgtca attacggtaa atggcccgcc tggctcaatg cccattgacg tcaataggac
      661 cacccaccat tgacgtcaat gggatggctc attgcccatt catatccgtt ctcacgcccc
      721 ctattgacgt caatgacggt aaatggccca cttggcagta catcaatatc tattaatagt
      781 aacttggcaa gtacattact attggaagta cgccagggta cattggcagt actcccattg
      841 acgtcaatgg cggtaaatgg cccgcgatgg ctgccaagta catccccatt gacgtcaatg
      901 gggaggggca atgacgcaaa tgggcgttcc attgacgtaa atgggcggta ggcgtgccta
      961 atgggaggtc tatataagca atgctcgttt agggaaccgc cattctgcct ggggacgtcg
     1021 gagcaagctt gatttaggtg acactataga cttgttcttt ttgcaggatc tgccgccacc
     1081 atggggatcc catcgatgtc gaccgaggag aatgtcaaga ggcgaacaca caacgtcttg
     1141 gagcgccaga ggaggaacga gctaaaacgg agcttttttg ccctgcgtga ccagatcccg
     1201 gagttggaaa acaatgaaaa ggcccccaag gtagttatcc ttaaaaaagc cacagcatac
     1261 atcctgtccg tccaagcaga gacgcaaaag ctcatttctg aaatcgactt gttgcggaaa
     1321 caaaacgaac agttgaaaca caaacttgaa cagctacgga actcttgtgc gtaagggatc
     1381 cccgggaatt caaggcctct cgagcctcta gaactatagt gagtcgtatt acgtagatcc
     1441 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     1501 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     1561 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     1621 ggaggttttt taattcgcgg ccgcggcgcc aatgcattgg gcccggtacc cagcttttgt
     1681 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     1741 tgaaattgtt atccgctcac aattccacac aacatacgag ccgggagcat aaagtgtaaa
     1801 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     1861 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     1921 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1981 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2041 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2101 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2161 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2221 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2281 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2341 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2401 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2461 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2521 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2581 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2641 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2701 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2761 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2821 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2881 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2941 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     3001 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     3061 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3121 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3181 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3241 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3301 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3361 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3421 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3481 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3541 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3601 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3661 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3721 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3781 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3841 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
     3901 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     3961 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     4021 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     4081 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     4141 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     4201 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     4261 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     4321 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     4381 cctcttc
//
